The Effect of Anti-aging Peptides on Mechanical and Biological Properties of HaCaT Keratinocytes
Atomic force microscopy (AFM) and fluorescence microscopy was applied to determine the influence of the anti-aging peptides on the morphology and the mechanical properties of keratinocytes. Immortalized human keratinocytes (HaCaT) were treated with two anti-aging bioactive peptides: Acetyl Tetrapept...
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| Veröffentlicht in: | International journal of peptide research and therapeutics 2018-12, Vol.24 (4), p.577-587 |
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| creator | Kobiela, Tomasz Milner-Krawczyk, Małgorzata Pasikowska-Piwko, Monika Bobecka-Wesołowska, Konstancja Eris, Irena Święszkowski, Wojciech Dulinska-Molak, Ida |
| description | Atomic force microscopy (AFM) and fluorescence microscopy was applied to determine the influence of the anti-aging peptides on the morphology and the mechanical properties of keratinocytes. Immortalized human keratinocytes (HaCaT) were treated with two anti-aging bioactive peptides: Acetyl Tetrapeptide-2 and Acetyl Hexapeptide-50 (Lipotec). The AFM measurement of the keratinocyte stiffness were carried after 48 h exposure at an indentation depth of 200 nm. AFM analysis showed increase of the cell stiffness for cells treated with Acetyl Tetrapeptide-2 (P1) in concentration range. Acetyl Hexapeptide-50 (P2) at concentration of 0.05 µg/ml also increased the stiffness of HaCaT cells but at higher concentrations 0.5 and 5 µg/ml cell stiffness was lower as compared to untreated control. Fluorescence microscopy revealed remodeling of actin filaments dependent on the concentration of P2 peptide. The mechanical response of HaCaT cells treated with P2 peptide corresponds to change of transcription level of ACTN1 and SOD2 which activity was expected to be modulated by P2 treatment. |
| doi_str_mv | 10.1007/s10989-017-9648-7 |
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Immortalized human keratinocytes (HaCaT) were treated with two anti-aging bioactive peptides: Acetyl Tetrapeptide-2 and Acetyl Hexapeptide-50 (Lipotec). The AFM measurement of the keratinocyte stiffness were carried after 48 h exposure at an indentation depth of 200 nm. AFM analysis showed increase of the cell stiffness for cells treated with Acetyl Tetrapeptide-2 (P1) in concentration range. Acetyl Hexapeptide-50 (P2) at concentration of 0.05 µg/ml also increased the stiffness of HaCaT cells but at higher concentrations 0.5 and 5 µg/ml cell stiffness was lower as compared to untreated control. Fluorescence microscopy revealed remodeling of actin filaments dependent on the concentration of P2 peptide. The mechanical response of HaCaT cells treated with P2 peptide corresponds to change of transcription level of ACTN1 and SOD2 which activity was expected to be modulated by P2 treatment.</description><identifier>ISSN: 1573-3149</identifier><identifier>EISSN: 1573-3904</identifier><identifier>DOI: 10.1007/s10989-017-9648-7</identifier><identifier>PMID: 30416406</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Actin ; Aging ; Animal Anatomy ; Atomic force microscopy ; Biochemistry ; Biological properties ; Biomedical and Life Sciences ; Filaments ; Fluorescence ; Fluorescence microscopy ; Histology ; Keratinocytes ; Life Sciences ; Mechanical properties ; Microscopy ; Molecular Medicine ; Morphology ; Peptides ; Pharmaceutical Sciences/Technology ; Pharmacology/Toxicology ; Polymer Sciences ; Superoxide dismutase ; Transcription</subject><ispartof>International journal of peptide research and therapeutics, 2018-12, Vol.24 (4), p.577-587</ispartof><rights>The Author(s) 2017</rights><rights>International Journal of Peptide Research and Therapeutics is a copyright of Springer, (2017). All Rights Reserved. © 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-87f760c79667adf5eaf0ab0bf5fcfa648cdeeeb04242101658cf8033dc6c118c3</citedby><cites>FETCH-LOGICAL-c470t-87f760c79667adf5eaf0ab0bf5fcfa648cdeeeb04242101658cf8033dc6c118c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10989-017-9648-7$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10989-017-9648-7$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30416406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kobiela, Tomasz</creatorcontrib><creatorcontrib>Milner-Krawczyk, Małgorzata</creatorcontrib><creatorcontrib>Pasikowska-Piwko, Monika</creatorcontrib><creatorcontrib>Bobecka-Wesołowska, Konstancja</creatorcontrib><creatorcontrib>Eris, Irena</creatorcontrib><creatorcontrib>Święszkowski, Wojciech</creatorcontrib><creatorcontrib>Dulinska-Molak, Ida</creatorcontrib><title>The Effect of Anti-aging Peptides on Mechanical and Biological Properties of HaCaT Keratinocytes</title><title>International journal of peptide research and therapeutics</title><addtitle>Int J Pept Res Ther</addtitle><addtitle>Int J Pept Res Ther</addtitle><description>Atomic force microscopy (AFM) and fluorescence microscopy was applied to determine the influence of the anti-aging peptides on the morphology and the mechanical properties of keratinocytes. Immortalized human keratinocytes (HaCaT) were treated with two anti-aging bioactive peptides: Acetyl Tetrapeptide-2 and Acetyl Hexapeptide-50 (Lipotec). The AFM measurement of the keratinocyte stiffness were carried after 48 h exposure at an indentation depth of 200 nm. AFM analysis showed increase of the cell stiffness for cells treated with Acetyl Tetrapeptide-2 (P1) in concentration range. Acetyl Hexapeptide-50 (P2) at concentration of 0.05 µg/ml also increased the stiffness of HaCaT cells but at higher concentrations 0.5 and 5 µg/ml cell stiffness was lower as compared to untreated control. Fluorescence microscopy revealed remodeling of actin filaments dependent on the concentration of P2 peptide. The mechanical response of HaCaT cells treated with P2 peptide corresponds to change of transcription level of ACTN1 and SOD2 which activity was expected to be modulated by P2 treatment.</description><subject>Actin</subject><subject>Aging</subject><subject>Animal Anatomy</subject><subject>Atomic force microscopy</subject><subject>Biochemistry</subject><subject>Biological properties</subject><subject>Biomedical and Life Sciences</subject><subject>Filaments</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Histology</subject><subject>Keratinocytes</subject><subject>Life Sciences</subject><subject>Mechanical properties</subject><subject>Microscopy</subject><subject>Molecular Medicine</subject><subject>Morphology</subject><subject>Peptides</subject><subject>Pharmaceutical Sciences/Technology</subject><subject>Pharmacology/Toxicology</subject><subject>Polymer Sciences</subject><subject>Superoxide dismutase</subject><subject>Transcription</subject><issn>1573-3149</issn><issn>1573-3904</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kc9vFCEUx4nR2B_6B3gxJF68jD4GBoaLSd201lhjD-sZWeYxSzMLK8ya9L-XddummngC8j7vC48PIa8YvGMA6n1hoHvdAFONlqJv1BNyzDrFG65BPL3fM6GPyEkpNwBdqxg8J0ccBJMC5DH5sVwjPfce3UyTp2dxDo0dQxzpNW7nMGChKdKv6NY2BmcnauNAP4Y0pfHP8TqnLeY57DlPL-3CLukXzHYOMbnbGcsL8szbqeDLu_WUfL84Xy4um6tvnz4vzq4aJxTMTa-8kuCUllLZwXdoPdgVrHznnbd1ODcg4gpEK1oGTHa98z1wPjjpGOsdPyUfDrnb3WqDg8M4ZzuZbQ4bm29NssH8XYlhbcb0y8gWeslFDXh7F5DTzx2W2WxCcThNNmLaFdMy3rYaNIOKvvkHvUm7HOt4hlURuv4t7yrFDpTLqZSM_uExDMzenzn4M9Wf2fszqva8fjzFQ8e9sAq0B6DUUhwxP7r6v6m_AY7ippQ</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Kobiela, Tomasz</creator><creator>Milner-Krawczyk, Małgorzata</creator><creator>Pasikowska-Piwko, Monika</creator><creator>Bobecka-Wesołowska, Konstancja</creator><creator>Eris, Irena</creator><creator>Święszkowski, Wojciech</creator><creator>Dulinska-Molak, Ida</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20181201</creationdate><title>The Effect of Anti-aging Peptides on Mechanical and Biological Properties of HaCaT Keratinocytes</title><author>Kobiela, Tomasz ; Milner-Krawczyk, Małgorzata ; Pasikowska-Piwko, Monika ; Bobecka-Wesołowska, Konstancja ; Eris, Irena ; Święszkowski, Wojciech ; Dulinska-Molak, Ida</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-87f760c79667adf5eaf0ab0bf5fcfa648cdeeeb04242101658cf8033dc6c118c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Actin</topic><topic>Aging</topic><topic>Animal Anatomy</topic><topic>Atomic force microscopy</topic><topic>Biochemistry</topic><topic>Biological properties</topic><topic>Biomedical and Life Sciences</topic><topic>Filaments</topic><topic>Fluorescence</topic><topic>Fluorescence microscopy</topic><topic>Histology</topic><topic>Keratinocytes</topic><topic>Life Sciences</topic><topic>Mechanical properties</topic><topic>Microscopy</topic><topic>Molecular Medicine</topic><topic>Morphology</topic><topic>Peptides</topic><topic>Pharmaceutical Sciences/Technology</topic><topic>Pharmacology/Toxicology</topic><topic>Polymer Sciences</topic><topic>Superoxide dismutase</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobiela, Tomasz</creatorcontrib><creatorcontrib>Milner-Krawczyk, Małgorzata</creatorcontrib><creatorcontrib>Pasikowska-Piwko, Monika</creatorcontrib><creatorcontrib>Bobecka-Wesołowska, Konstancja</creatorcontrib><creatorcontrib>Eris, Irena</creatorcontrib><creatorcontrib>Święszkowski, Wojciech</creatorcontrib><creatorcontrib>Dulinska-Molak, Ida</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of peptide research and therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobiela, Tomasz</au><au>Milner-Krawczyk, Małgorzata</au><au>Pasikowska-Piwko, Monika</au><au>Bobecka-Wesołowska, Konstancja</au><au>Eris, Irena</au><au>Święszkowski, Wojciech</au><au>Dulinska-Molak, Ida</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Anti-aging Peptides on Mechanical and Biological Properties of HaCaT Keratinocytes</atitle><jtitle>International journal of peptide research and therapeutics</jtitle><stitle>Int J Pept Res Ther</stitle><addtitle>Int J Pept Res Ther</addtitle><date>2018-12-01</date><risdate>2018</risdate><volume>24</volume><issue>4</issue><spage>577</spage><epage>587</epage><pages>577-587</pages><issn>1573-3149</issn><eissn>1573-3904</eissn><abstract>Atomic force microscopy (AFM) and fluorescence microscopy was applied to determine the influence of the anti-aging peptides on the morphology and the mechanical properties of keratinocytes. Immortalized human keratinocytes (HaCaT) were treated with two anti-aging bioactive peptides: Acetyl Tetrapeptide-2 and Acetyl Hexapeptide-50 (Lipotec). The AFM measurement of the keratinocyte stiffness were carried after 48 h exposure at an indentation depth of 200 nm. AFM analysis showed increase of the cell stiffness for cells treated with Acetyl Tetrapeptide-2 (P1) in concentration range. Acetyl Hexapeptide-50 (P2) at concentration of 0.05 µg/ml also increased the stiffness of HaCaT cells but at higher concentrations 0.5 and 5 µg/ml cell stiffness was lower as compared to untreated control. Fluorescence microscopy revealed remodeling of actin filaments dependent on the concentration of P2 peptide. The mechanical response of HaCaT cells treated with P2 peptide corresponds to change of transcription level of ACTN1 and SOD2 which activity was expected to be modulated by P2 treatment.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30416406</pmid><doi>10.1007/s10989-017-9648-7</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actin Aging Animal Anatomy Atomic force microscopy Biochemistry Biological properties Biomedical and Life Sciences Filaments Fluorescence Fluorescence microscopy Histology Keratinocytes Life Sciences Mechanical properties Microscopy Molecular Medicine Morphology Peptides Pharmaceutical Sciences/Technology Pharmacology/Toxicology Polymer Sciences Superoxide dismutase Transcription |
| title | The Effect of Anti-aging Peptides on Mechanical and Biological Properties of HaCaT Keratinocytes |
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