Isolation of Escherichia coli carrying the blaCTX-M-1 and qnrS1 genes from reproductive organs of broiler breeders and internal contents of hatching eggs
This study aimed to characterize two third-generation cephalosporins- and quinolone-resistant Escherichia coli (TGCs- and Q-R-Ec) isolates recovered from the ovaries of a broiler breeder flock and the internal contents of hatching eggs produced by the broiler breeder flock. Clonal relatedness was de...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2018, Vol.80(10), pp.1540-1543 |
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| creator | BENAMEUR, Qada TALI-MAAMAR, Hassiba ASSAOUS, Farida GUETTOU, Badia TAHRAT, Naϊma AGGOUNE, Nadjet RAHAL, Kheira BEN-MAHDI, Meriem-Hind |
| description | This study aimed to characterize two third-generation cephalosporins- and quinolone-resistant Escherichia coli (TGCs- and Q-R-Ec) isolates recovered from the ovaries of a broiler breeder flock and the internal contents of hatching eggs produced by the broiler breeder flock. Clonal relatedness was determined by multilocus sequence typing (MLST). The isolates displayed the same multidrug resistance profile, with resistance to ampicillin, ticarcillin, piperacillin, cefazollin, cephalothin, cefotaxime, nalidixic acid, tetracycline and sulfonamides. Double disk synergy test demonstrated that the two isolates presented an ESBL phenotype. PCR and sequencing results showed that both the isolates harbored the blaCTX-M-1 and qnrS1 genes. MLST revealed a novel allele combination, designated as ST461, in these isolates. This study would contribute to the molecular epidemiological understanding of TGCs- and/or Q-R-Ec. |
| doi_str_mv | 10.1292/jvms.18-0283 |
| format | Article |
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Clonal relatedness was determined by multilocus sequence typing (MLST). The isolates displayed the same multidrug resistance profile, with resistance to ampicillin, ticarcillin, piperacillin, cefazollin, cephalothin, cefotaxime, nalidixic acid, tetracycline and sulfonamides. Double disk synergy test demonstrated that the two isolates presented an ESBL phenotype. PCR and sequencing results showed that both the isolates harbored the blaCTX-M-1 and qnrS1 genes. MLST revealed a novel allele combination, designated as ST461, in these isolates. This study would contribute to the molecular epidemiological understanding of TGCs- and/or Q-R-Ec.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.18-0283</identifier><identifier>PMID: 30175752</identifier><language>eng</language><publisher>Tokyo: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Ampicillin ; antimicrobial resistance ; Avian Pathology ; Bacteria ; Cefotaxime ; Cephalosporins ; Cephalothin ; E coli ; Eggs ; Epidemiology ; Escherichia coli ; Hatching ; molecular epidemiology ; Multidrug resistance ; Multilocus sequence typing ; Nalidixic acid ; Organs ; Ovaries ; ovary ; PCR ; Phenotypes ; Piperacillin ; poultry ; Reproductive organs ; Sulfonamides</subject><ispartof>Journal of Veterinary Medical Science, 2018, Vol.80(10), pp.1540-1543</ispartof><rights>2018 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency Oct 2018</rights><rights>2018 The Japanese Society of Veterinary Science 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4236-3dd0f90a5cbc5cda48e24c7e7649aae73e40a2d75547be65889d7bce4eb1a1213</citedby><cites>FETCH-LOGICAL-c4236-3dd0f90a5cbc5cda48e24c7e7649aae73e40a2d75547be65889d7bce4eb1a1213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207526/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207526/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,1877,4010,27904,27905,27906,53772,53774</link.rule.ids></links><search><creatorcontrib>BENAMEUR, Qada</creatorcontrib><creatorcontrib>TALI-MAAMAR, Hassiba</creatorcontrib><creatorcontrib>ASSAOUS, Farida</creatorcontrib><creatorcontrib>GUETTOU, Badia</creatorcontrib><creatorcontrib>TAHRAT, Naϊma</creatorcontrib><creatorcontrib>AGGOUNE, Nadjet</creatorcontrib><creatorcontrib>RAHAL, Kheira</creatorcontrib><creatorcontrib>BEN-MAHDI, Meriem-Hind</creatorcontrib><title>Isolation of Escherichia coli carrying the blaCTX-M-1 and qnrS1 genes from reproductive organs of broiler breeders and internal contents of hatching eggs</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>This study aimed to characterize two third-generation cephalosporins- and quinolone-resistant Escherichia coli (TGCs- and Q-R-Ec) isolates recovered from the ovaries of a broiler breeder flock and the internal contents of hatching eggs produced by the broiler breeder flock. Clonal relatedness was determined by multilocus sequence typing (MLST). The isolates displayed the same multidrug resistance profile, with resistance to ampicillin, ticarcillin, piperacillin, cefazollin, cephalothin, cefotaxime, nalidixic acid, tetracycline and sulfonamides. Double disk synergy test demonstrated that the two isolates presented an ESBL phenotype. PCR and sequencing results showed that both the isolates harbored the blaCTX-M-1 and qnrS1 genes. MLST revealed a novel allele combination, designated as ST461, in these isolates. This study would contribute to the molecular epidemiological understanding of TGCs- and/or Q-R-Ec.</description><subject>Ampicillin</subject><subject>antimicrobial resistance</subject><subject>Avian Pathology</subject><subject>Bacteria</subject><subject>Cefotaxime</subject><subject>Cephalosporins</subject><subject>Cephalothin</subject><subject>E coli</subject><subject>Eggs</subject><subject>Epidemiology</subject><subject>Escherichia coli</subject><subject>Hatching</subject><subject>molecular epidemiology</subject><subject>Multidrug resistance</subject><subject>Multilocus sequence typing</subject><subject>Nalidixic acid</subject><subject>Organs</subject><subject>Ovaries</subject><subject>ovary</subject><subject>PCR</subject><subject>Phenotypes</subject><subject>Piperacillin</subject><subject>poultry</subject><subject>Reproductive organs</subject><subject>Sulfonamides</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkc9u1DAQxiMEokvhxgNY4sKBFP9LnFyQ0KpApSIOFImbNXEmiVeJvbWTlfoovC1OU60EF4-l-X3fN5rJsreMXjFe84-H0xSvWJVTXoln2Y4JqXIlRf0829GalbniBb3IXsV4oJQzWdYvswtBmSpUwXfZn5voR5itd8R35DqaAYM1gwVi_GiJgRAerOvJPCBpRtjf_c6_54yAa8m9Cz8Z6dFhJF3wEwl4DL5dzGxPSHzowcXVtAnejhhSRWwxxEexdTMGB2OKST83P5IDzCk6pWHfx9fZiw7GiG-e6mX268v13f5bfvvj683-821uJBdlLtqWdjWFwjSmMC3ICrk0ClUpawBUAiUF3qqikKrBsqiqulWNQYkNA8aZuMw-bb7HpZmwNWmYAKM-BjtBeNAerP634-yge3_SJadphWUyeP9kEPz9gnHWk40GxxEc-iVqTuuaSi6lSOi7_9CDX9Y1JIqLumQFrVSiPmyUCT7GgN15GEb1enO93lyzSq83T_h-ww9xhh7PMITZmhE3uKKreH031blrBgganfgL_3W5ew</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>BENAMEUR, Qada</creator><creator>TALI-MAAMAR, Hassiba</creator><creator>ASSAOUS, Farida</creator><creator>GUETTOU, Badia</creator><creator>TAHRAT, Naϊma</creator><creator>AGGOUNE, Nadjet</creator><creator>RAHAL, Kheira</creator><creator>BEN-MAHDI, Meriem-Hind</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2018</creationdate><title>Isolation of Escherichia coli carrying the blaCTX-M-1 and qnrS1 genes from reproductive organs of broiler breeders and internal contents of hatching eggs</title><author>BENAMEUR, Qada ; TALI-MAAMAR, Hassiba ; ASSAOUS, Farida ; GUETTOU, Badia ; TAHRAT, Naϊma ; AGGOUNE, Nadjet ; RAHAL, Kheira ; BEN-MAHDI, Meriem-Hind</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4236-3dd0f90a5cbc5cda48e24c7e7649aae73e40a2d75547be65889d7bce4eb1a1213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Ampicillin</topic><topic>antimicrobial resistance</topic><topic>Avian Pathology</topic><topic>Bacteria</topic><topic>Cefotaxime</topic><topic>Cephalosporins</topic><topic>Cephalothin</topic><topic>E coli</topic><topic>Eggs</topic><topic>Epidemiology</topic><topic>Escherichia coli</topic><topic>Hatching</topic><topic>molecular epidemiology</topic><topic>Multidrug resistance</topic><topic>Multilocus sequence typing</topic><topic>Nalidixic acid</topic><topic>Organs</topic><topic>Ovaries</topic><topic>ovary</topic><topic>PCR</topic><topic>Phenotypes</topic><topic>Piperacillin</topic><topic>poultry</topic><topic>Reproductive organs</topic><topic>Sulfonamides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BENAMEUR, Qada</creatorcontrib><creatorcontrib>TALI-MAAMAR, Hassiba</creatorcontrib><creatorcontrib>ASSAOUS, Farida</creatorcontrib><creatorcontrib>GUETTOU, Badia</creatorcontrib><creatorcontrib>TAHRAT, Naϊma</creatorcontrib><creatorcontrib>AGGOUNE, Nadjet</creatorcontrib><creatorcontrib>RAHAL, Kheira</creatorcontrib><creatorcontrib>BEN-MAHDI, Meriem-Hind</creatorcontrib><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BENAMEUR, Qada</au><au>TALI-MAAMAR, Hassiba</au><au>ASSAOUS, Farida</au><au>GUETTOU, Badia</au><au>TAHRAT, Naϊma</au><au>AGGOUNE, Nadjet</au><au>RAHAL, Kheira</au><au>BEN-MAHDI, Meriem-Hind</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Escherichia coli carrying the blaCTX-M-1 and qnrS1 genes from reproductive organs of broiler breeders and internal contents of hatching eggs</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2018</date><risdate>2018</risdate><volume>80</volume><issue>10</issue><spage>1540</spage><epage>1543</epage><pages>1540-1543</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>This study aimed to characterize two third-generation cephalosporins- and quinolone-resistant Escherichia coli (TGCs- and Q-R-Ec) isolates recovered from the ovaries of a broiler breeder flock and the internal contents of hatching eggs produced by the broiler breeder flock. Clonal relatedness was determined by multilocus sequence typing (MLST). The isolates displayed the same multidrug resistance profile, with resistance to ampicillin, ticarcillin, piperacillin, cefazollin, cephalothin, cefotaxime, nalidixic acid, tetracycline and sulfonamides. Double disk synergy test demonstrated that the two isolates presented an ESBL phenotype. PCR and sequencing results showed that both the isolates harbored the blaCTX-M-1 and qnrS1 genes. MLST revealed a novel allele combination, designated as ST461, in these isolates. This study would contribute to the molecular epidemiological understanding of TGCs- and/or Q-R-Ec.</abstract><cop>Tokyo</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>30175752</pmid><doi>10.1292/jvms.18-0283</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Ampicillin antimicrobial resistance Avian Pathology Bacteria Cefotaxime Cephalosporins Cephalothin E coli Eggs Epidemiology Escherichia coli Hatching molecular epidemiology Multidrug resistance Multilocus sequence typing Nalidixic acid Organs Ovaries ovary PCR Phenotypes Piperacillin poultry Reproductive organs Sulfonamides |
| title | Isolation of Escherichia coli carrying the blaCTX-M-1 and qnrS1 genes from reproductive organs of broiler breeders and internal contents of hatching eggs |
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