Cell Transport Prompts the Performance of Low-Voltage Electroporation for Cell Inactivation
The inactivation of pathogens in liquids has broad applications, ranging from water disinfection to food pasteurization. However, common cell inactivation methods ( e.g ., chlorination, ultraviolet radiation and thermal treatment) have significant drawbacks such as carcinogenic byproduct formation,...
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| Veröffentlicht in: | Scientific reports 2018-10, Vol.8 (1), p.15832-10, Article 15832 |
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| creator | Huo, Zheng-Yang Li, Guo-Qiang Yu, Tong Feng, Chao Lu, Yun Wu, Yin-Hu Yu, Cecilia Xie, Xing Hu, Hong-Ying |
| description | The inactivation of pathogens in liquids has broad applications, ranging from water disinfection to food pasteurization. However, common cell inactivation methods (
e.g
., chlorination, ultraviolet radiation and thermal treatment) have significant drawbacks such as carcinogenic byproduct formation, energy intensiveness and/or nutrient structure destruction. Here, we fabricated a new approach to address these challenges by applying a low-voltage electroporation disinfection cell (EDC) and investigate the critical mechanisms of cell transport to allow high inactivation performance. The EDC prototypes were equipped with two one-dimensional (1D) nanostructure-assisted electrodes that enabled high electric field strength (>107 V m
−1
) near the electrode surface with a low applied voltage (1 V). We have identified that during electroporation disinfection, electrophoresis, dielectrophoresis and hydraulic flow are the three major mechanisms which transport cells into the vicinity of the electrode surface to achieve superior disinfection performance. The EDC treated 70 ml of bacteria sample with an initial cell concentration of 10
7
CFU ml
−1
and achieved complete bacteria inactivation (survival rate |
| doi_str_mv | 10.1038/s41598-018-34027-0 |
| format | Article |
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e.g
., chlorination, ultraviolet radiation and thermal treatment) have significant drawbacks such as carcinogenic byproduct formation, energy intensiveness and/or nutrient structure destruction. Here, we fabricated a new approach to address these challenges by applying a low-voltage electroporation disinfection cell (EDC) and investigate the critical mechanisms of cell transport to allow high inactivation performance. The EDC prototypes were equipped with two one-dimensional (1D) nanostructure-assisted electrodes that enabled high electric field strength (>107 V m
−1
) near the electrode surface with a low applied voltage (1 V). We have identified that during electroporation disinfection, electrophoresis, dielectrophoresis and hydraulic flow are the three major mechanisms which transport cells into the vicinity of the electrode surface to achieve superior disinfection performance. The EDC treated 70 ml of bacteria sample with an initial cell concentration of 10
7
CFU ml
−1
and achieved complete bacteria inactivation (survival rate <0.00001%; no live bacteria detected). Our findings will help to establish a foundation for the future development and implementation of low-voltage electroporation for cell inactivation.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-34027-0</identifier><identifier>PMID: 30361540</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>639/301/357/1016 ; 704/172/169/896 ; 704/172/4081 ; Bacteria ; Disinfection ; Electrodes ; Electroporation ; Humanities and Social Sciences ; multidisciplinary ; Pasteurization ; Science ; Science (multidisciplinary) ; Ultraviolet radiation ; Voltage</subject><ispartof>Scientific reports, 2018-10, Vol.8 (1), p.15832-10, Article 15832</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-308b1ed3a53a04742e2d63741aae98522f9a9107accd0e2974437394d97679ae3</citedby><cites>FETCH-LOGICAL-c513t-308b1ed3a53a04742e2d63741aae98522f9a9107accd0e2974437394d97679ae3</cites><orcidid>0000-0002-2253-0964</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202345/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202345/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27922,27923,41118,42187,51574,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30361540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huo, Zheng-Yang</creatorcontrib><creatorcontrib>Li, Guo-Qiang</creatorcontrib><creatorcontrib>Yu, Tong</creatorcontrib><creatorcontrib>Feng, Chao</creatorcontrib><creatorcontrib>Lu, Yun</creatorcontrib><creatorcontrib>Wu, Yin-Hu</creatorcontrib><creatorcontrib>Yu, Cecilia</creatorcontrib><creatorcontrib>Xie, Xing</creatorcontrib><creatorcontrib>Hu, Hong-Ying</creatorcontrib><title>Cell Transport Prompts the Performance of Low-Voltage Electroporation for Cell Inactivation</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The inactivation of pathogens in liquids has broad applications, ranging from water disinfection to food pasteurization. However, common cell inactivation methods (
e.g
., chlorination, ultraviolet radiation and thermal treatment) have significant drawbacks such as carcinogenic byproduct formation, energy intensiveness and/or nutrient structure destruction. Here, we fabricated a new approach to address these challenges by applying a low-voltage electroporation disinfection cell (EDC) and investigate the critical mechanisms of cell transport to allow high inactivation performance. The EDC prototypes were equipped with two one-dimensional (1D) nanostructure-assisted electrodes that enabled high electric field strength (>107 V m
−1
) near the electrode surface with a low applied voltage (1 V). We have identified that during electroporation disinfection, electrophoresis, dielectrophoresis and hydraulic flow are the three major mechanisms which transport cells into the vicinity of the electrode surface to achieve superior disinfection performance. The EDC treated 70 ml of bacteria sample with an initial cell concentration of 10
7
CFU ml
−1
and achieved complete bacteria inactivation (survival rate <0.00001%; no live bacteria detected). Our findings will help to establish a foundation for the future development and implementation of low-voltage electroporation for cell inactivation.</description><subject>639/301/357/1016</subject><subject>704/172/169/896</subject><subject>704/172/4081</subject><subject>Bacteria</subject><subject>Disinfection</subject><subject>Electrodes</subject><subject>Electroporation</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Pasteurization</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Ultraviolet radiation</subject><subject>Voltage</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcFPHCEUxknTRo31H-ihIemll7HwgGG4NGk2tppsUg_qpQeCzJt1zAxsgdX0vy_uWrU9lAuE93vfe18-Qt5xdsyZ6D5lyZXpGsa7RkgGumGvyAEwqRoQAK9fvPfJUc63rB4FRnKzR_YFEy1Xkh2QHwucJnqRXMjrmAo9T3Fel0zLDdJzTENMswseaRzoMt43V3EqboX0ZEJfUqwtrowx0MrRrdJZcL6Md9vft-TN4KaMR4_3Ibn8enKxOG2W37-dLb4sG6-4KI1g3TXHXjglHJNaAkLfCi25c2g6BTAYZzjTzvueIRgtpdDCyN7oVhuH4pB83umuN9cz9h5DSW6y6zTOLv2y0Y3270oYb-wq3tkWGAipqsDHR4EUf24wFzuP2Vc7LmDcZAsc2rpBHV3RD_-gt3GTQrX3QCnQ0EFbKdhRPsWcEw5Py3BmH-Kzu_hsjc9u47OsNr1_aeOp5U9YFRA7INdSWGF6nv0f2d_hCqVx</recordid><startdate>20181025</startdate><enddate>20181025</enddate><creator>Huo, Zheng-Yang</creator><creator>Li, Guo-Qiang</creator><creator>Yu, Tong</creator><creator>Feng, Chao</creator><creator>Lu, Yun</creator><creator>Wu, Yin-Hu</creator><creator>Yu, Cecilia</creator><creator>Xie, Xing</creator><creator>Hu, Hong-Ying</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2253-0964</orcidid></search><sort><creationdate>20181025</creationdate><title>Cell Transport Prompts the Performance of Low-Voltage Electroporation for Cell Inactivation</title><author>Huo, Zheng-Yang ; 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However, common cell inactivation methods (
e.g
., chlorination, ultraviolet radiation and thermal treatment) have significant drawbacks such as carcinogenic byproduct formation, energy intensiveness and/or nutrient structure destruction. Here, we fabricated a new approach to address these challenges by applying a low-voltage electroporation disinfection cell (EDC) and investigate the critical mechanisms of cell transport to allow high inactivation performance. The EDC prototypes were equipped with two one-dimensional (1D) nanostructure-assisted electrodes that enabled high electric field strength (>107 V m
−1
) near the electrode surface with a low applied voltage (1 V). We have identified that during electroporation disinfection, electrophoresis, dielectrophoresis and hydraulic flow are the three major mechanisms which transport cells into the vicinity of the electrode surface to achieve superior disinfection performance. The EDC treated 70 ml of bacteria sample with an initial cell concentration of 10
7
CFU ml
−1
and achieved complete bacteria inactivation (survival rate <0.00001%; no live bacteria detected). Our findings will help to establish a foundation for the future development and implementation of low-voltage electroporation for cell inactivation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30361540</pmid><doi>10.1038/s41598-018-34027-0</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-2253-0964</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 639/301/357/1016 704/172/169/896 704/172/4081 Bacteria Disinfection Electrodes Electroporation Humanities and Social Sciences multidisciplinary Pasteurization Science Science (multidisciplinary) Ultraviolet radiation Voltage |
| title | Cell Transport Prompts the Performance of Low-Voltage Electroporation for Cell Inactivation |
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