LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21
Long non‐coding RNAs (lncRNAs) have emerged as important regulators of cancer, including colorectal cancer (CRC). The exact expression pattern of long intergenic noncoding RNA 00312 (LINC00312) in CRC and its mechanisms of action have not been reported. Here, we found that LINC00312 is underexpresse...
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| Veröffentlicht in: | Journal of cellular and molecular medicine 2018-11, Vol.22 (11), p.5565-5572 |
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| creator | Li, Gang Wang, Changming Wang, Yongbing Xu, Bin Zhang, Wenzhong |
| description | Long non‐coding RNAs (lncRNAs) have emerged as important regulators of cancer, including colorectal cancer (CRC). The exact expression pattern of long intergenic noncoding RNA 00312 (LINC00312) in CRC and its mechanisms of action have not been reported. Here, we found that LINC00312 is underexpressed in CRC tissues and cell lines. Functional experiments suggested that LINC00312 suppresses growth, migration and invasion of CRC cells in vitro and attenuates tumour proliferation and metastasis in vivo. Mechanistically, LINC00312 was found to regulate the malignancy of CRC cells by binding to miR‐21 and by functioning as a tumour suppressor targeting PTEN. Overexpression of miR‐21 or knockdown of PTEN attenuated the LINC00312‐mediated inhibition of CRC cell proliferation and invasion. Taken together, our results elucidate the role of the LINC00312–miR‐21–PTEN axis in CRC cell proliferation and tumour progression and may lead to new lncRNA‐based diagnostics or therapeutics for CRC. |
| doi_str_mv | 10.1111/jcmm.13830 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6201213</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2092541039</sourcerecordid><originalsourceid>FETCH-LOGICAL-j4100-da015dc2809db8571aab0c901ce4be4216601d049064037f382d9a87e01add5e3</originalsourceid><addsrcrecordid>eNpVUU1PGzEQtSpQ-eqFH1D52EvojL272b1UqqICqQJIiJ4trz0BR951am-KcuMn8Bv5JXVIisrI0jxr3rwZ-zF2inCGOb4uTNedoawlfGCHWNZiVDSy2NthrGV9wI5SWgDICmXzkR1IQFnk6yGj2fR6khEKHmkZKSVKfBmDd3OKenCh57q3vKNBp3xc4mHOTfAhkhm050b3hiI35H3i7TqL3K_8ti8TO3f78vQs8ITtz7VP9GmXj9mv8x93k8vR7OZiOvk-Gy0KBBhZDVhaI2pobFuXY9S6BdMAGipaKgRWFaCFooGqADmey1rYRtdjAtTWliSP2bet7nLVdmQN9UPUXi2j63Rcq6Cdel_p3YO6D39UJQAFyizwZScQw-8VpUF1Lm0ep3sKq6QENKLMu8omUz__P-ttyL-_zQTcEh6dp_VbHUFtXFMb19Sra-rn5OrqFcm_DESLcQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2092541039</pqid></control><display><type>article</type><title>LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21</title><source>MEDLINE</source><source>Wiley Online Library</source><source>Wiley Online Library Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>EZB Electronic Journals Library</source><creator>Li, Gang ; Wang, Changming ; Wang, Yongbing ; Xu, Bin ; Zhang, Wenzhong</creator><creatorcontrib>Li, Gang ; Wang, Changming ; Wang, Yongbing ; Xu, Bin ; Zhang, Wenzhong</creatorcontrib><description>Long non‐coding RNAs (lncRNAs) have emerged as important regulators of cancer, including colorectal cancer (CRC). The exact expression pattern of long intergenic noncoding RNA 00312 (LINC00312) in CRC and its mechanisms of action have not been reported. Here, we found that LINC00312 is underexpressed in CRC tissues and cell lines. Functional experiments suggested that LINC00312 suppresses growth, migration and invasion of CRC cells in vitro and attenuates tumour proliferation and metastasis in vivo. Mechanistically, LINC00312 was found to regulate the malignancy of CRC cells by binding to miR‐21 and by functioning as a tumour suppressor targeting PTEN. Overexpression of miR‐21 or knockdown of PTEN attenuated the LINC00312‐mediated inhibition of CRC cell proliferation and invasion. Taken together, our results elucidate the role of the LINC00312–miR‐21–PTEN axis in CRC cell proliferation and tumour progression and may lead to new lncRNA‐based diagnostics or therapeutics for CRC.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/jcmm.13830</identifier><identifier>PMID: 30134003</identifier><language>eng</language><publisher>England: John Wiley and Sons Inc</publisher><subject>Animals ; Cell Movement - genetics ; Cell Proliferation - genetics ; colorectal cancer ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Gene Expression Regulation, Neoplastic ; Heterografts ; HT29 Cells ; Humans ; LINC00312 ; metastasis ; Mice ; MicroRNAs - genetics ; miR‐21 ; Neoplasm Invasiveness - genetics ; Neoplasm Invasiveness - pathology ; Neoplasm Metastasis ; Original ; PTEN ; PTEN Phosphohydrolase - genetics ; RNA, Long Noncoding - genetics</subject><ispartof>Journal of cellular and molecular medicine, 2018-11, Vol.22 (11), p.5565-5572</ispartof><rights>2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-0453-6294</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201213/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201213/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30134003$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Gang</creatorcontrib><creatorcontrib>Wang, Changming</creatorcontrib><creatorcontrib>Wang, Yongbing</creatorcontrib><creatorcontrib>Xu, Bin</creatorcontrib><creatorcontrib>Zhang, Wenzhong</creatorcontrib><title>LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21</title><title>Journal of cellular and molecular medicine</title><addtitle>J Cell Mol Med</addtitle><description>Long non‐coding RNAs (lncRNAs) have emerged as important regulators of cancer, including colorectal cancer (CRC). The exact expression pattern of long intergenic noncoding RNA 00312 (LINC00312) in CRC and its mechanisms of action have not been reported. Here, we found that LINC00312 is underexpressed in CRC tissues and cell lines. Functional experiments suggested that LINC00312 suppresses growth, migration and invasion of CRC cells in vitro and attenuates tumour proliferation and metastasis in vivo. Mechanistically, LINC00312 was found to regulate the malignancy of CRC cells by binding to miR‐21 and by functioning as a tumour suppressor targeting PTEN. Overexpression of miR‐21 or knockdown of PTEN attenuated the LINC00312‐mediated inhibition of CRC cell proliferation and invasion. Taken together, our results elucidate the role of the LINC00312–miR‐21–PTEN axis in CRC cell proliferation and tumour progression and may lead to new lncRNA‐based diagnostics or therapeutics for CRC.</description><subject>Animals</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>colorectal cancer</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Heterografts</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>LINC00312</subject><subject>metastasis</subject><subject>Mice</subject><subject>MicroRNAs - genetics</subject><subject>miR‐21</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplasm Invasiveness - pathology</subject><subject>Neoplasm Metastasis</subject><subject>Original</subject><subject>PTEN</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>RNA, Long Noncoding - genetics</subject><issn>1582-1838</issn><issn>1582-4934</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNpVUU1PGzEQtSpQ-eqFH1D52EvojL272b1UqqICqQJIiJ4trz0BR951am-KcuMn8Bv5JXVIisrI0jxr3rwZ-zF2inCGOb4uTNedoawlfGCHWNZiVDSy2NthrGV9wI5SWgDICmXzkR1IQFnk6yGj2fR6khEKHmkZKSVKfBmDd3OKenCh57q3vKNBp3xc4mHOTfAhkhm050b3hiI35H3i7TqL3K_8ti8TO3f78vQs8ITtz7VP9GmXj9mv8x93k8vR7OZiOvk-Gy0KBBhZDVhaI2pobFuXY9S6BdMAGipaKgRWFaCFooGqADmey1rYRtdjAtTWliSP2bet7nLVdmQN9UPUXi2j63Rcq6Cdel_p3YO6D39UJQAFyizwZScQw-8VpUF1Lm0ep3sKq6QENKLMu8omUz__P-ttyL-_zQTcEh6dp_VbHUFtXFMb19Sra-rn5OrqFcm_DESLcQ</recordid><startdate>201811</startdate><enddate>201811</enddate><creator>Li, Gang</creator><creator>Wang, Changming</creator><creator>Wang, Yongbing</creator><creator>Xu, Bin</creator><creator>Zhang, Wenzhong</creator><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0453-6294</orcidid></search><sort><creationdate>201811</creationdate><title>LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21</title><author>Li, Gang ; Wang, Changming ; Wang, Yongbing ; Xu, Bin ; Zhang, Wenzhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j4100-da015dc2809db8571aab0c901ce4be4216601d049064037f382d9a87e01add5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>colorectal cancer</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Heterografts</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>LINC00312</topic><topic>metastasis</topic><topic>Mice</topic><topic>MicroRNAs - genetics</topic><topic>miR‐21</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Neoplasm Invasiveness - pathology</topic><topic>Neoplasm Metastasis</topic><topic>Original</topic><topic>PTEN</topic><topic>PTEN Phosphohydrolase - genetics</topic><topic>RNA, Long Noncoding - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Gang</creatorcontrib><creatorcontrib>Wang, Changming</creatorcontrib><creatorcontrib>Wang, Yongbing</creatorcontrib><creatorcontrib>Xu, Bin</creatorcontrib><creatorcontrib>Zhang, Wenzhong</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cellular and molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Gang</au><au>Wang, Changming</au><au>Wang, Yongbing</au><au>Xu, Bin</au><au>Zhang, Wenzhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21</atitle><jtitle>Journal of cellular and molecular medicine</jtitle><addtitle>J Cell Mol Med</addtitle><date>2018-11</date><risdate>2018</risdate><volume>22</volume><issue>11</issue><spage>5565</spage><epage>5572</epage><pages>5565-5572</pages><issn>1582-1838</issn><eissn>1582-4934</eissn><abstract>Long non‐coding RNAs (lncRNAs) have emerged as important regulators of cancer, including colorectal cancer (CRC). The exact expression pattern of long intergenic noncoding RNA 00312 (LINC00312) in CRC and its mechanisms of action have not been reported. Here, we found that LINC00312 is underexpressed in CRC tissues and cell lines. Functional experiments suggested that LINC00312 suppresses growth, migration and invasion of CRC cells in vitro and attenuates tumour proliferation and metastasis in vivo. Mechanistically, LINC00312 was found to regulate the malignancy of CRC cells by binding to miR‐21 and by functioning as a tumour suppressor targeting PTEN. Overexpression of miR‐21 or knockdown of PTEN attenuated the LINC00312‐mediated inhibition of CRC cell proliferation and invasion. Taken together, our results elucidate the role of the LINC00312–miR‐21–PTEN axis in CRC cell proliferation and tumour progression and may lead to new lncRNA‐based diagnostics or therapeutics for CRC.</abstract><cop>England</cop><pub>John Wiley and Sons Inc</pub><pmid>30134003</pmid><doi>10.1111/jcmm.13830</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-0453-6294</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cell Movement - genetics Cell Proliferation - genetics colorectal cancer Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Gene Expression Regulation, Neoplastic Heterografts HT29 Cells Humans LINC00312 metastasis Mice MicroRNAs - genetics miR‐21 Neoplasm Invasiveness - genetics Neoplasm Invasiveness - pathology Neoplasm Metastasis Original PTEN PTEN Phosphohydrolase - genetics RNA, Long Noncoding - genetics |
| title | LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21 |
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