Molecular detection of Nosema ceranae in the apiaries of Kurdistan province, Iran
Nosema disease is one of the most important diseases of adult honey bees worldwide. It is known as silent killer because there are no characteristic symptoms. The aim of the present study was to determine prevalence of species in various towns of Kurdistan province in Iran. A multiplex polymerase ch...
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| Veröffentlicht in: | Veterinary research forum 2018, Vol.9 (3), p.273-278 |
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| creator | Khezri, Mohammad Moharrami, Mojtaba Modirrousta, Hossain Torkaman, Maryam Salehi, Saleh Rokhzad, Babak Khanbabai, Homan |
| description | Nosema disease is one of the most important diseases of adult honey bees worldwide. It is known as silent killer because there are no characteristic symptoms. The aim of the present study was to determine prevalence of
species in various towns of Kurdistan province in Iran. A multiplex polymerase chain reaction (multiplex-PCR) was performed for identification of
species infecting European honeybee,
. A total of 100 samples were collected from apiaries (870 hives) in 10 counties of Kurdistan province, located in the west of Iran. Samples were examined using light microscope and PCR. The light microscope was used to determine the presence of
spores in all of the collected samples. Multiplex-PCR based on 16S ribosomal RNA was used to differentiate
from
. Overall prevalence of the microscopic evaluation and PCR method were 29.00% and 32.00%, respectively. The analysis of Nosema isolates from interrogation of DNA databank entries of Kurdistan apiaries (based on rRNA sequence data) indicated that only
was widespread in these apiaries, and it had already been found in high percentages (50.00%) in Marivan and Kamiaran counties of Kurdistan province. It was shown that only
was found by PCR assay in the region. |
| doi_str_mv | 10.30466/vrf.2018.32086 |
| format | Article |
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species in various towns of Kurdistan province in Iran. A multiplex polymerase chain reaction (multiplex-PCR) was performed for identification of
species infecting European honeybee,
. A total of 100 samples were collected from apiaries (870 hives) in 10 counties of Kurdistan province, located in the west of Iran. Samples were examined using light microscope and PCR. The light microscope was used to determine the presence of
spores in all of the collected samples. Multiplex-PCR based on 16S ribosomal RNA was used to differentiate
from
. Overall prevalence of the microscopic evaluation and PCR method were 29.00% and 32.00%, respectively. The analysis of Nosema isolates from interrogation of DNA databank entries of Kurdistan apiaries (based on rRNA sequence data) indicated that only
was widespread in these apiaries, and it had already been found in high percentages (50.00%) in Marivan and Kamiaran counties of Kurdistan province. It was shown that only
was found by PCR assay in the region.</description><identifier>ISSN: 2008-8140</identifier><identifier>EISSN: 2322-3618</identifier><identifier>DOI: 10.30466/vrf.2018.32086</identifier><identifier>PMID: 30357093</identifier><language>eng</language><publisher>Iran: Veterinary Research Forum</publisher><subject>Animal behavior ; Bees ; Deoxyribonucleic acid ; Disease ; DNA ; Infections ; Parasites ; Pathogens ; Polymerase chain reaction ; Protozoa ; Short Communication</subject><ispartof>Veterinary research forum, 2018, Vol.9 (3), p.273-278</ispartof><rights>2018. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Urmia University. All rights reserved.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198153/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198153/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,4012,27910,27911,27912,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30357093$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khezri, Mohammad</creatorcontrib><creatorcontrib>Moharrami, Mojtaba</creatorcontrib><creatorcontrib>Modirrousta, Hossain</creatorcontrib><creatorcontrib>Torkaman, Maryam</creatorcontrib><creatorcontrib>Salehi, Saleh</creatorcontrib><creatorcontrib>Rokhzad, Babak</creatorcontrib><creatorcontrib>Khanbabai, Homan</creatorcontrib><title>Molecular detection of Nosema ceranae in the apiaries of Kurdistan province, Iran</title><title>Veterinary research forum</title><addtitle>Vet Res Forum</addtitle><description>Nosema disease is one of the most important diseases of adult honey bees worldwide. It is known as silent killer because there are no characteristic symptoms. The aim of the present study was to determine prevalence of
species in various towns of Kurdistan province in Iran. A multiplex polymerase chain reaction (multiplex-PCR) was performed for identification of
species infecting European honeybee,
. A total of 100 samples were collected from apiaries (870 hives) in 10 counties of Kurdistan province, located in the west of Iran. Samples were examined using light microscope and PCR. The light microscope was used to determine the presence of
spores in all of the collected samples. Multiplex-PCR based on 16S ribosomal RNA was used to differentiate
from
. Overall prevalence of the microscopic evaluation and PCR method were 29.00% and 32.00%, respectively. The analysis of Nosema isolates from interrogation of DNA databank entries of Kurdistan apiaries (based on rRNA sequence data) indicated that only
was widespread in these apiaries, and it had already been found in high percentages (50.00%) in Marivan and Kamiaran counties of Kurdistan province. It was shown that only
was found by PCR assay in the region.</description><subject>Animal behavior</subject><subject>Bees</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>DNA</subject><subject>Infections</subject><subject>Parasites</subject><subject>Pathogens</subject><subject>Polymerase chain reaction</subject><subject>Protozoa</subject><subject>Short Communication</subject><issn>2008-8140</issn><issn>2322-3618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpVkEtLw0AURgdRbKldu5MBt6bOO5ONIMVH8YWg63AzmbEj7UycJAX_vSlW0dW9cD_O_TgIHVMy40Qodb5JbsYI1TPOiFZ7aMw4YxlXVO8POyE601SQEZq2ra-IELkSktNDNOKEy5wUfIyeH-LKmn4FCde2s6bzMeDo8GNs7RqwsQkCWOwD7pYWQ-MhedtuE3d9qn3bQcBNihsfjD3DiyF9hA4crFo73c0Jer2-epnfZvdPN4v55X3WsEJ0WU6lqnKliOGVU5QraZhSSlBiNWNSqIIbl9cAFS9k7WqXUycBpJWkFkN_PkEX39ymr9a2NjZ0CVZlk_wa0mcZwZf_L8Evy7e4KRUtNJVbwOkOkOJHb9uufI99CkPnklGqB5fFoGiCTv6--eX_KORfyph0ZQ</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Khezri, Mohammad</creator><creator>Moharrami, Mojtaba</creator><creator>Modirrousta, Hossain</creator><creator>Torkaman, Maryam</creator><creator>Salehi, Saleh</creator><creator>Rokhzad, Babak</creator><creator>Khanbabai, Homan</creator><general>Veterinary Research Forum</general><general>Urmia University Press</general><scope>NPM</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>2018</creationdate><title>Molecular detection of Nosema ceranae in the apiaries of Kurdistan province, Iran</title><author>Khezri, Mohammad ; Moharrami, Mojtaba ; Modirrousta, Hossain ; Torkaman, Maryam ; Salehi, Saleh ; Rokhzad, Babak ; Khanbabai, Homan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p294t-7156b7660c3bf61365c2666410e82254693cf7daab395dfdf71f5aa5e50d43033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal behavior</topic><topic>Bees</topic><topic>Deoxyribonucleic acid</topic><topic>Disease</topic><topic>DNA</topic><topic>Infections</topic><topic>Parasites</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>Protozoa</topic><topic>Short Communication</topic><toplevel>online_resources</toplevel><creatorcontrib>Khezri, Mohammad</creatorcontrib><creatorcontrib>Moharrami, Mojtaba</creatorcontrib><creatorcontrib>Modirrousta, Hossain</creatorcontrib><creatorcontrib>Torkaman, Maryam</creatorcontrib><creatorcontrib>Salehi, Saleh</creatorcontrib><creatorcontrib>Rokhzad, Babak</creatorcontrib><creatorcontrib>Khanbabai, Homan</creatorcontrib><collection>PubMed</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Veterinary research forum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khezri, Mohammad</au><au>Moharrami, Mojtaba</au><au>Modirrousta, Hossain</au><au>Torkaman, Maryam</au><au>Salehi, Saleh</au><au>Rokhzad, Babak</au><au>Khanbabai, Homan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular detection of Nosema ceranae in the apiaries of Kurdistan province, Iran</atitle><jtitle>Veterinary research forum</jtitle><addtitle>Vet Res Forum</addtitle><date>2018</date><risdate>2018</risdate><volume>9</volume><issue>3</issue><spage>273</spage><epage>278</epage><pages>273-278</pages><issn>2008-8140</issn><eissn>2322-3618</eissn><abstract>Nosema disease is one of the most important diseases of adult honey bees worldwide. It is known as silent killer because there are no characteristic symptoms. The aim of the present study was to determine prevalence of
species in various towns of Kurdistan province in Iran. A multiplex polymerase chain reaction (multiplex-PCR) was performed for identification of
species infecting European honeybee,
. A total of 100 samples were collected from apiaries (870 hives) in 10 counties of Kurdistan province, located in the west of Iran. Samples were examined using light microscope and PCR. The light microscope was used to determine the presence of
spores in all of the collected samples. Multiplex-PCR based on 16S ribosomal RNA was used to differentiate
from
. Overall prevalence of the microscopic evaluation and PCR method were 29.00% and 32.00%, respectively. The analysis of Nosema isolates from interrogation of DNA databank entries of Kurdistan apiaries (based on rRNA sequence data) indicated that only
was widespread in these apiaries, and it had already been found in high percentages (50.00%) in Marivan and Kamiaran counties of Kurdistan province. It was shown that only
was found by PCR assay in the region.</abstract><cop>Iran</cop><pub>Veterinary Research Forum</pub><pmid>30357093</pmid><doi>10.30466/vrf.2018.32086</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animal behavior Bees Deoxyribonucleic acid Disease DNA Infections Parasites Pathogens Polymerase chain reaction Protozoa Short Communication |
| title | Molecular detection of Nosema ceranae in the apiaries of Kurdistan province, Iran |
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