A DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells
Here, we have developed a localized catalytic hairpin assembly (LCHA) strategy for intracellular miR-21 imaging by using DNA nanowires confining both hairpin probes in a compact space. The LCHA is constructed by interval hybridization of DNA hairpin probe pairs to a DNA nanowire with multiplex footh...
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| Veröffentlicht in: | Chemical science (Cambridge) 2018-10, Vol.9 (40), p.7802-7808 |
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| creator | Wei, Qiaomei Huang, Jin Li, Jing Wang, Jiaoli Yang, Xiaohai Liu, Jianbo Wang, Kemin |
| description | Here, we have developed a localized catalytic hairpin assembly (LCHA) strategy for intracellular miR-21 imaging by using DNA nanowires confining both hairpin probes in a compact space. The LCHA is constructed by interval hybridization of DNA hairpin probe pairs to a DNA nanowire with multiplex footholds generated by alternating chain hybridization. Compared to the conventional catalytic hairpin assembly (CHA) strategy, the LCHA significantly shortens the reaction time and enhances the sensitivity. Moreover, the proposed LCHA can serve as a carrier for delivery of probes into live cells as well as protect the probes from nuclease degradation and enhances the stability. We anticipate that this design can be widely applied in facilitating basic biomedical research and disease diagnosis. |
| doi_str_mv | 10.1039/c8sc02943a |
| format | Article |
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We anticipate that this design can be widely applied in facilitating basic biomedical research and disease diagnosis.</description><subject>Assembly</subject><subject>Catalysis</subject><subject>Chemistry</subject><subject>Confining</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Medical imaging</subject><subject>Nanowires</subject><subject>Nuclease</subject><subject>Reaction time</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sensitivity enhancement</subject><issn>2041-6520</issn><issn>2041-6539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkd1LHDEUxUNpUVFf_ANKoC8irOZjZnbyUljW-gFSwepzuJO5WSOZZJvMWta_3ljtos3LvZAfh3PPIeSAs2POpDoxbTZMqErCJ7IjWMUnTS3V580u2DbZz_mBlSclr8V0i2xLVgmlWrVDcEZPf85ogBD_uIS0g4w99dGAd09lMzCCX4_O0HtwaekChZxx6PyaJgQzuhiojYkOzqR4U5TcAAsXFrSQ3j0iNeh93iNfLPiM-29zl9yd_bidX0yurs8v57OriammYpxMrWmMbVndd03TCikY9KA4t9gzhrIVKMpgNWu6up3WqPpGcrCtslz1VW3lLvn-qrtcdQP2BsOYwOtlKq7SWkdw-uNPcPd6ER91w1VVKVUEDt8EUvy9wjzqweWXEyBgXGUtuCw2ZKNYQb_9hz7EVQrlvEIJXtIvIRfq6JUq8eSc0G7McKZfCtTz9tf8b4GzAn99b3-D_qtLPgMsFJYT</recordid><startdate>20181028</startdate><enddate>20181028</enddate><creator>Wei, Qiaomei</creator><creator>Huang, Jin</creator><creator>Li, Jing</creator><creator>Wang, Jiaoli</creator><creator>Yang, Xiaohai</creator><creator>Liu, Jianbo</creator><creator>Wang, Kemin</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9390-4938</orcidid><orcidid>https://orcid.org/0000-0001-8122-7140</orcidid><orcidid>https://orcid.org/0000-0002-2890-682X</orcidid></search><sort><creationdate>20181028</creationdate><title>A DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells</title><author>Wei, Qiaomei ; Huang, Jin ; Li, Jing ; Wang, Jiaoli ; Yang, Xiaohai ; Liu, Jianbo ; Wang, Kemin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-7fc6cf805db6682320ada911fed00e382e20e30506b5875e9d631af89f19d45f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Assembly</topic><topic>Catalysis</topic><topic>Chemistry</topic><topic>Confining</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Medical imaging</topic><topic>Nanowires</topic><topic>Nuclease</topic><topic>Reaction time</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Sensitivity enhancement</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Qiaomei</creatorcontrib><creatorcontrib>Huang, Jin</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Wang, Jiaoli</creatorcontrib><creatorcontrib>Yang, Xiaohai</creatorcontrib><creatorcontrib>Liu, Jianbo</creatorcontrib><creatorcontrib>Wang, Kemin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chemical science (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Qiaomei</au><au>Huang, Jin</au><au>Li, Jing</au><au>Wang, Jiaoli</au><au>Yang, Xiaohai</au><au>Liu, Jianbo</au><au>Wang, Kemin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells</atitle><jtitle>Chemical science (Cambridge)</jtitle><addtitle>Chem Sci</addtitle><date>2018-10-28</date><risdate>2018</risdate><volume>9</volume><issue>40</issue><spage>7802</spage><epage>7808</epage><pages>7802-7808</pages><issn>2041-6520</issn><eissn>2041-6539</eissn><abstract>Here, we have developed a localized catalytic hairpin assembly (LCHA) strategy for intracellular miR-21 imaging by using DNA nanowires confining both hairpin probes in a compact space. 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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Assembly Catalysis Chemistry Confining Deoxyribonucleic acid DNA Medical imaging Nanowires Nuclease Reaction time Ribonucleic acid RNA Sensitivity enhancement |
| title | A DNA nanowire based localized catalytic hairpin assembly reaction for microRNA imaging in live cells |
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