Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro
Objective Salmonella enterica remains a major cause of food-borne disease in humans, and Salmonella Typhimurium (ST) contamination of poultry products is a worldwide problem. Since macrophages play an essential role in controlling Salmonella infection, the aim of this study was to evaluate the effec...
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| Veröffentlicht in: | Journal of Zhejiang University. B. Science 2018-10, Vol.19 (10), p.785-795 |
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| creator | Wang, Bai-kui Mao, Yu-long Gong, Li Xu, Xin Jiang, Shou-qun Wang, Yi-bing Li, Wei-fen |
| description | Objective
Salmonella enterica
remains a major cause of food-borne disease in humans, and
Salmonella
Typhimurium (ST) contamination of poultry products is a worldwide problem. Since macrophages play an essential role in controlling
Salmonella
infection, the aim of this study was to evaluate the effect of glycyrrhizic acid (GA) on immune function of chicken HD11 macrophages.
Methods
Chicken HD11 macrophages were treated with GA (0, 12.5, 25, 50, 100, 200, 400, or 800 μg/ml) and lipopolysaccharide (LPS, 500 ng/ml) for 3, 6, 12, 24, or 48 h. Evaluated responses included phagocytosis, bacteria-killing, gene expression of cell surface molecules (cluster of differentiation 40 (
CD40
),
CD80
,
CD83
, and
CD197
) and antimicrobial effectors (inducible nitric oxide synthase (
iNOS
), NADPH oxidase-1 (
NOX-1
), interferon-γ (
IFN-γ
), LPS-induced tumor necrosis factor (TNF)-α factor (LITAF), interleukin-6 (
IL-6
), and
IL-10
), and production of nitric oxide (NO) and hydrogen peroxide (H
2
O
2
).
Results
GA increased the internalization of both fluorescein isothiocyanate (FITC)-dextran and ST by HD11 cells and markedly decreased the intracellular survival of ST. We found that the messenger RNA (mRNA) expression of cell surface molecules (
CD40
,
CD80
,
CD83
, and
CD197
) and cytokines (
IFN-γ
, I
L-6
, and
IL-10
) of HD11 cells was up-regulated following GA exposure. The expression of
iNOS
and
NOX-1
was induced by GA and thereby the productions of NO and H
2
O
2
in HD11 cells were enhanced. Notably, it was verified that nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) pathways were responsible for GA-induced synthesis of NO and
IFN-γ
gene expression.
Conclusions
Taken together, these results suggested that GA exhibits a potent immune regulatory effect to activate chicken macrophages and enhances
Salmonella
-killing capacity. |
| doi_str_mv | 10.1631/jzus.B1700506 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6194354</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2115269470</sourcerecordid><originalsourceid>FETCH-LOGICAL-c487t-cc9109794f17b963f8ba3db5ac1935e0894a1add683678cfb97f755014d5e3c93</originalsourceid><addsrcrecordid>eNqFkUtv1DAUhS0Eog9YskWR2LDJ4Bu_N0i0ghapEgtgbTmOk3iaOIOdjDT99TiatjyExMav--lc33MQegV4A5zAu-3dkjYXIDBmmD9BpyB5VYKQ5Gk-c0FKYBJO0FlKW4wpxYI_RycEV1xRyk9RdzUc7CHG3t95Wxjrm7zMfm9mlwrbe3vrQjEaG6ddb7r8ZkJTuNCbYPNl7p2PxVczjFNww2DKWz8MPnSFNbusNR8KH4q9n-P0Aj1rzZDcy_v9HH3_9PHb5XV58-Xq8-WHm9JSKebSWgVYCUVbELXipJW1IU3NjAVFmMNSUQOmabgkXEjb1kq0gjEMtGGOWEXO0fuj7m6pR9dYF-ZoBr2LfjTxoCfj9Z-V4HvdTXvNQVHCaBZ4ey8Qpx-LS7MefbLrcMFNS9JVVQGmBCryfxSArT4LnNE3f6HbaYkhO7FSSmKhhMhUeaSy3SlF1z7-G7Be09Zr2voh7cy__n3YR_oh3gxsjkDKpdC5-KvtvxV_Aiybtxg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2119807977</pqid></control><display><type>article</type><title>Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>SpringerLink Journals - AutoHoldings</source><creator>Wang, Bai-kui ; Mao, Yu-long ; Gong, Li ; Xu, Xin ; Jiang, Shou-qun ; Wang, Yi-bing ; Li, Wei-fen</creator><creatorcontrib>Wang, Bai-kui ; Mao, Yu-long ; Gong, Li ; Xu, Xin ; Jiang, Shou-qun ; Wang, Yi-bing ; Li, Wei-fen</creatorcontrib><description>Objective
Salmonella enterica
remains a major cause of food-borne disease in humans, and
Salmonella
Typhimurium (ST) contamination of poultry products is a worldwide problem. Since macrophages play an essential role in controlling
Salmonella
infection, the aim of this study was to evaluate the effect of glycyrrhizic acid (GA) on immune function of chicken HD11 macrophages.
Methods
Chicken HD11 macrophages were treated with GA (0, 12.5, 25, 50, 100, 200, 400, or 800 μg/ml) and lipopolysaccharide (LPS, 500 ng/ml) for 3, 6, 12, 24, or 48 h. Evaluated responses included phagocytosis, bacteria-killing, gene expression of cell surface molecules (cluster of differentiation 40 (
CD40
),
CD80
,
CD83
, and
CD197
) and antimicrobial effectors (inducible nitric oxide synthase (
iNOS
), NADPH oxidase-1 (
NOX-1
), interferon-γ (
IFN-γ
), LPS-induced tumor necrosis factor (TNF)-α factor (LITAF), interleukin-6 (
IL-6
), and
IL-10
), and production of nitric oxide (NO) and hydrogen peroxide (H
2
O
2
).
Results
GA increased the internalization of both fluorescein isothiocyanate (FITC)-dextran and ST by HD11 cells and markedly decreased the intracellular survival of ST. We found that the messenger RNA (mRNA) expression of cell surface molecules (
CD40
,
CD80
,
CD83
, and
CD197
) and cytokines (
IFN-γ
, I
L-6
, and
IL-10
) of HD11 cells was up-regulated following GA exposure. The expression of
iNOS
and
NOX-1
was induced by GA and thereby the productions of NO and H
2
O
2
in HD11 cells were enhanced. Notably, it was verified that nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) pathways were responsible for GA-induced synthesis of NO and
IFN-γ
gene expression.
Conclusions
Taken together, these results suggested that GA exhibits a potent immune regulatory effect to activate chicken macrophages and enhances
Salmonella
-killing capacity.</description><identifier>ISSN: 1673-1581</identifier><identifier>EISSN: 1862-1783</identifier><identifier>DOI: 10.1631/jzus.B1700506</identifier><identifier>PMID: 30269446</identifier><language>eng</language><publisher>Hangzhou: Zhejiang University Press</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; c-Jun protein ; CCR7 receptor ; CD40 antigen ; CD80 antigen ; CD83 antigen ; Cell surface ; Cells, Cultured ; Chickens ; Contamination ; Cytokines ; Dextran ; Fluorescein ; Fluorescein isothiocyanate ; Food contamination ; Foodborne diseases ; foodborne illness ; Gene expression ; gene expression regulation ; Glycyrrhizic Acid - pharmacology ; glycyrrhizin ; humans ; Hydrogen peroxide ; Immune response ; inducible nitric oxide synthase ; Interferon ; interferon-gamma ; Interleukin 10 ; Interleukin 6 ; Internalization ; isothiocyanates ; JNK protein ; Kinases ; Lipopolysaccharides ; Macrophage Activation - drug effects ; Macrophages ; messenger RNA ; mitogen-activated protein kinase ; NAD(P)H oxidase ; NAD(P)H oxidase (H2O2-forming) ; NF-kappa B - physiology ; NF-κB protein ; Nitric oxide ; Phagocytosis ; Phagocytosis - drug effects ; Poultry ; poultry products ; Ribonucleic acid ; RNA ; Salmonella ; Salmonella - drug effects ; Salmonella enterica ; Salmonella Typhimurium ; salmonellosis ; Signal Transduction - drug effects ; transcription factor NF-kappa B ; Transcription factors ; tumor necrosis factor-alpha ; Tumor necrosis factor-TNF ; γ-Interferon</subject><ispartof>Journal of Zhejiang University. B. Science, 2018-10, Vol.19 (10), p.785-795</ispartof><rights>Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Journal of Zhejiang University-SCIENCE B is a copyright of Springer, (2018). All Rights Reserved.</rights><rights>Copyright © Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2018 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-cc9109794f17b963f8ba3db5ac1935e0894a1add683678cfb97f755014d5e3c93</citedby><cites>FETCH-LOGICAL-c487t-cc9109794f17b963f8ba3db5ac1935e0894a1add683678cfb97f755014d5e3c93</cites><orcidid>0000-0001-8159-9876</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6194354/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6194354/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,41469,42538,51300,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30269446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Bai-kui</creatorcontrib><creatorcontrib>Mao, Yu-long</creatorcontrib><creatorcontrib>Gong, Li</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><creatorcontrib>Jiang, Shou-qun</creatorcontrib><creatorcontrib>Wang, Yi-bing</creatorcontrib><creatorcontrib>Li, Wei-fen</creatorcontrib><title>Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro</title><title>Journal of Zhejiang University. B. Science</title><addtitle>J. Zhejiang Univ. Sci. B</addtitle><addtitle>J Zhejiang Univ Sci B</addtitle><description>Objective
Salmonella enterica
remains a major cause of food-borne disease in humans, and
Salmonella
Typhimurium (ST) contamination of poultry products is a worldwide problem. Since macrophages play an essential role in controlling
Salmonella
infection, the aim of this study was to evaluate the effect of glycyrrhizic acid (GA) on immune function of chicken HD11 macrophages.
Methods
Chicken HD11 macrophages were treated with GA (0, 12.5, 25, 50, 100, 200, 400, or 800 μg/ml) and lipopolysaccharide (LPS, 500 ng/ml) for 3, 6, 12, 24, or 48 h. Evaluated responses included phagocytosis, bacteria-killing, gene expression of cell surface molecules (cluster of differentiation 40 (
CD40
),
CD80
,
CD83
, and
CD197
) and antimicrobial effectors (inducible nitric oxide synthase (
iNOS
), NADPH oxidase-1 (
NOX-1
), interferon-γ (
IFN-γ
), LPS-induced tumor necrosis factor (TNF)-α factor (LITAF), interleukin-6 (
IL-6
), and
IL-10
), and production of nitric oxide (NO) and hydrogen peroxide (H
2
O
2
).
Results
GA increased the internalization of both fluorescein isothiocyanate (FITC)-dextran and ST by HD11 cells and markedly decreased the intracellular survival of ST. We found that the messenger RNA (mRNA) expression of cell surface molecules (
CD40
,
CD80
,
CD83
, and
CD197
) and cytokines (
IFN-γ
, I
L-6
, and
IL-10
) of HD11 cells was up-regulated following GA exposure. The expression of
iNOS
and
NOX-1
was induced by GA and thereby the productions of NO and H
2
O
2
in HD11 cells were enhanced. Notably, it was verified that nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) pathways were responsible for GA-induced synthesis of NO and
IFN-γ
gene expression.
Conclusions
Taken together, these results suggested that GA exhibits a potent immune regulatory effect to activate chicken macrophages and enhances
Salmonella
-killing capacity.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>c-Jun protein</subject><subject>CCR7 receptor</subject><subject>CD40 antigen</subject><subject>CD80 antigen</subject><subject>CD83 antigen</subject><subject>Cell surface</subject><subject>Cells, Cultured</subject><subject>Chickens</subject><subject>Contamination</subject><subject>Cytokines</subject><subject>Dextran</subject><subject>Fluorescein</subject><subject>Fluorescein isothiocyanate</subject><subject>Food contamination</subject><subject>Foodborne diseases</subject><subject>foodborne illness</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Glycyrrhizic Acid - pharmacology</subject><subject>glycyrrhizin</subject><subject>humans</subject><subject>Hydrogen peroxide</subject><subject>Immune response</subject><subject>inducible nitric oxide synthase</subject><subject>Interferon</subject><subject>interferon-gamma</subject><subject>Interleukin 10</subject><subject>Interleukin 6</subject><subject>Internalization</subject><subject>isothiocyanates</subject><subject>JNK protein</subject><subject>Kinases</subject><subject>Lipopolysaccharides</subject><subject>Macrophage Activation - drug effects</subject><subject>Macrophages</subject><subject>messenger RNA</subject><subject>mitogen-activated protein kinase</subject><subject>NAD(P)H oxidase</subject><subject>NAD(P)H oxidase (H2O2-forming)</subject><subject>NF-kappa B - physiology</subject><subject>NF-κB protein</subject><subject>Nitric oxide</subject><subject>Phagocytosis</subject><subject>Phagocytosis - drug effects</subject><subject>Poultry</subject><subject>poultry products</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Salmonella</subject><subject>Salmonella - drug effects</subject><subject>Salmonella enterica</subject><subject>Salmonella Typhimurium</subject><subject>salmonellosis</subject><subject>Signal Transduction - drug effects</subject><subject>transcription factor NF-kappa B</subject><subject>Transcription factors</subject><subject>tumor necrosis factor-alpha</subject><subject>Tumor necrosis factor-TNF</subject><subject>γ-Interferon</subject><issn>1673-1581</issn><issn>1862-1783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUtv1DAUhS0Eog9YskWR2LDJ4Bu_N0i0ghapEgtgbTmOk3iaOIOdjDT99TiatjyExMav--lc33MQegV4A5zAu-3dkjYXIDBmmD9BpyB5VYKQ5Gk-c0FKYBJO0FlKW4wpxYI_RycEV1xRyk9RdzUc7CHG3t95Wxjrm7zMfm9mlwrbe3vrQjEaG6ddb7r8ZkJTuNCbYPNl7p2PxVczjFNww2DKWz8MPnSFNbusNR8KH4q9n-P0Aj1rzZDcy_v9HH3_9PHb5XV58-Xq8-WHm9JSKebSWgVYCUVbELXipJW1IU3NjAVFmMNSUQOmabgkXEjb1kq0gjEMtGGOWEXO0fuj7m6pR9dYF-ZoBr2LfjTxoCfj9Z-V4HvdTXvNQVHCaBZ4ey8Qpx-LS7MefbLrcMFNS9JVVQGmBCryfxSArT4LnNE3f6HbaYkhO7FSSmKhhMhUeaSy3SlF1z7-G7Be09Zr2voh7cy__n3YR_oh3gxsjkDKpdC5-KvtvxV_Aiybtxg</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Wang, Bai-kui</creator><creator>Mao, Yu-long</creator><creator>Gong, Li</creator><creator>Xu, Xin</creator><creator>Jiang, Shou-qun</creator><creator>Wang, Yi-bing</creator><creator>Li, Wei-fen</creator><general>Zhejiang University Press</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8159-9876</orcidid></search><sort><creationdate>20181001</creationdate><title>Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro</title><author>Wang, Bai-kui ; Mao, Yu-long ; Gong, Li ; Xu, Xin ; Jiang, Shou-qun ; Wang, Yi-bing ; Li, Wei-fen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-cc9109794f17b963f8ba3db5ac1935e0894a1add683678cfb97f755014d5e3c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>c-Jun protein</topic><topic>CCR7 receptor</topic><topic>CD40 antigen</topic><topic>CD80 antigen</topic><topic>CD83 antigen</topic><topic>Cell surface</topic><topic>Cells, Cultured</topic><topic>Chickens</topic><topic>Contamination</topic><topic>Cytokines</topic><topic>Dextran</topic><topic>Fluorescein</topic><topic>Fluorescein isothiocyanate</topic><topic>Food contamination</topic><topic>Foodborne diseases</topic><topic>foodborne illness</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>Glycyrrhizic Acid - pharmacology</topic><topic>glycyrrhizin</topic><topic>humans</topic><topic>Hydrogen peroxide</topic><topic>Immune response</topic><topic>inducible nitric oxide synthase</topic><topic>Interferon</topic><topic>interferon-gamma</topic><topic>Interleukin 10</topic><topic>Interleukin 6</topic><topic>Internalization</topic><topic>isothiocyanates</topic><topic>JNK protein</topic><topic>Kinases</topic><topic>Lipopolysaccharides</topic><topic>Macrophage Activation - drug effects</topic><topic>Macrophages</topic><topic>messenger RNA</topic><topic>mitogen-activated protein kinase</topic><topic>NAD(P)H oxidase</topic><topic>NAD(P)H oxidase (H2O2-forming)</topic><topic>NF-kappa B - physiology</topic><topic>NF-κB protein</topic><topic>Nitric oxide</topic><topic>Phagocytosis</topic><topic>Phagocytosis - drug effects</topic><topic>Poultry</topic><topic>poultry products</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Salmonella</topic><topic>Salmonella - drug effects</topic><topic>Salmonella enterica</topic><topic>Salmonella Typhimurium</topic><topic>salmonellosis</topic><topic>Signal Transduction - drug effects</topic><topic>transcription factor NF-kappa B</topic><topic>Transcription factors</topic><topic>tumor necrosis factor-alpha</topic><topic>Tumor necrosis factor-TNF</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Bai-kui</creatorcontrib><creatorcontrib>Mao, Yu-long</creatorcontrib><creatorcontrib>Gong, Li</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><creatorcontrib>Jiang, Shou-qun</creatorcontrib><creatorcontrib>Wang, Yi-bing</creatorcontrib><creatorcontrib>Li, Wei-fen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Zhejiang University. B. Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Bai-kui</au><au>Mao, Yu-long</au><au>Gong, Li</au><au>Xu, Xin</au><au>Jiang, Shou-qun</au><au>Wang, Yi-bing</au><au>Li, Wei-fen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro</atitle><jtitle>Journal of Zhejiang University. B. Science</jtitle><stitle>J. Zhejiang Univ. Sci. B</stitle><addtitle>J Zhejiang Univ Sci B</addtitle><date>2018-10-01</date><risdate>2018</risdate><volume>19</volume><issue>10</issue><spage>785</spage><epage>795</epage><pages>785-795</pages><issn>1673-1581</issn><eissn>1862-1783</eissn><abstract>Objective
Salmonella enterica
remains a major cause of food-borne disease in humans, and
Salmonella
Typhimurium (ST) contamination of poultry products is a worldwide problem. Since macrophages play an essential role in controlling
Salmonella
infection, the aim of this study was to evaluate the effect of glycyrrhizic acid (GA) on immune function of chicken HD11 macrophages.
Methods
Chicken HD11 macrophages were treated with GA (0, 12.5, 25, 50, 100, 200, 400, or 800 μg/ml) and lipopolysaccharide (LPS, 500 ng/ml) for 3, 6, 12, 24, or 48 h. Evaluated responses included phagocytosis, bacteria-killing, gene expression of cell surface molecules (cluster of differentiation 40 (
CD40
),
CD80
,
CD83
, and
CD197
) and antimicrobial effectors (inducible nitric oxide synthase (
iNOS
), NADPH oxidase-1 (
NOX-1
), interferon-γ (
IFN-γ
), LPS-induced tumor necrosis factor (TNF)-α factor (LITAF), interleukin-6 (
IL-6
), and
IL-10
), and production of nitric oxide (NO) and hydrogen peroxide (H
2
O
2
).
Results
GA increased the internalization of both fluorescein isothiocyanate (FITC)-dextran and ST by HD11 cells and markedly decreased the intracellular survival of ST. We found that the messenger RNA (mRNA) expression of cell surface molecules (
CD40
,
CD80
,
CD83
, and
CD197
) and cytokines (
IFN-γ
, I
L-6
, and
IL-10
) of HD11 cells was up-regulated following GA exposure. The expression of
iNOS
and
NOX-1
was induced by GA and thereby the productions of NO and H
2
O
2
in HD11 cells were enhanced. Notably, it was verified that nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) pathways were responsible for GA-induced synthesis of NO and
IFN-γ
gene expression.
Conclusions
Taken together, these results suggested that GA exhibits a potent immune regulatory effect to activate chicken macrophages and enhances
Salmonella
-killing capacity.</abstract><cop>Hangzhou</cop><pub>Zhejiang University Press</pub><pmid>30269446</pmid><doi>10.1631/jzus.B1700506</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8159-9876</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1673-1581 |
| ispartof | Journal of Zhejiang University. B. Science, 2018-10, Vol.19 (10), p.785-795 |
| issn | 1673-1581 1862-1783 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6194354 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biomedical and Life Sciences Biomedicine c-Jun protein CCR7 receptor CD40 antigen CD80 antigen CD83 antigen Cell surface Cells, Cultured Chickens Contamination Cytokines Dextran Fluorescein Fluorescein isothiocyanate Food contamination Foodborne diseases foodborne illness Gene expression gene expression regulation Glycyrrhizic Acid - pharmacology glycyrrhizin humans Hydrogen peroxide Immune response inducible nitric oxide synthase Interferon interferon-gamma Interleukin 10 Interleukin 6 Internalization isothiocyanates JNK protein Kinases Lipopolysaccharides Macrophage Activation - drug effects Macrophages messenger RNA mitogen-activated protein kinase NAD(P)H oxidase NAD(P)H oxidase (H2O2-forming) NF-kappa B - physiology NF-κB protein Nitric oxide Phagocytosis Phagocytosis - drug effects Poultry poultry products Ribonucleic acid RNA Salmonella Salmonella - drug effects Salmonella enterica Salmonella Typhimurium salmonellosis Signal Transduction - drug effects transcription factor NF-kappa B Transcription factors tumor necrosis factor-alpha Tumor necrosis factor-TNF γ-Interferon |
| title | Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T23%3A20%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glycyrrhizic%20acid%20activates%20chicken%20macrophages%20and%20enhances%20their%20Salmonella-killing%20capacity%20in%20vitro&rft.jtitle=Journal%20of%20Zhejiang%20University.%20B.%20Science&rft.au=Wang,%20Bai-kui&rft.date=2018-10-01&rft.volume=19&rft.issue=10&rft.spage=785&rft.epage=795&rft.pages=785-795&rft.issn=1673-1581&rft.eissn=1862-1783&rft_id=info:doi/10.1631/jzus.B1700506&rft_dat=%3Cproquest_pubme%3E2115269470%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2119807977&rft_id=info:pmid/30269446&rfr_iscdi=true |