Mechanisms of precipitate formation during the purification of an Fc‐fusion protein

Protein precipitates that arise during bioprocessing can cause manufacturing challenges, but they can also aid in clearance of host‐cell protein (HCP) and DNA impurities. Such precipitates differ from many protein precipitates that have been studied previously in their heterogeneous composition, par...

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Veröffentlicht in:	Biotechnology and bioengineering 2018-10, Vol.115 (10), p.2489-2503
Hauptverfasser:	Greene, Daniel G., Traylor, Steven J., Guo, Jing, Choe, Leila H., Modla, Shannon, Xu, Xuankuo, Singh, Nripen, Lock, Lye Lin, Ghose, Sanchayita, Li, Zheng Jian, Lee, Kelvin H., Wagner, Norman J., Lenhoff, Abraham M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid Animals Bioprocessing Chemical precipitation CHO Cells Citric acid Cricetinae Cricetulus Deactivation Deoxyribonucleic acid DNA dynamic light scattering Electrostatic properties Fc receptors fractal dimension Fractals Fractional Precipitation Fusion protein host‐cell protein Immunoglobulin Fc Fragments - biosynthesis Immunoglobulin Fc Fragments - chemistry Immunoglobulin Fc Fragments - isolation & purification Impurities Inactivation Ionic strength Light scattering Microscopy Microstructure Models, Chemical Neutralization Optical microscopy particle size distribution Particulates pH effects Photon correlation spectroscopy Precipitates Protein A protein precipitation Protein purification Proteins Proteomics Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - isolation & purification Scattering Time dependence Transmission electron microscopy
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