Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d

CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease...

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Veröffentlicht in:	Cell 2018-09, Vol.175 (1), p.212-223.e17
Hauptverfasser:	Zhang, Cheng, Konermann, Silvana, Brideau, Nicholas J., Lotfy, Peter, Wu, Xuebing, Novick, Scott J., Strutzenberg, Timothy, Griffin, Patrick R., Hsu, Patrick D., Lyumkis, Dmitry
Format:	Artikel
Sprache:	eng
Schlagworte:	Cas13 Cas13d Cas9 Clustered Regularly Interspaced Short Palindromic Repeats - genetics CRISPR CRISPR-Cas Systems - genetics CRISPR-Cas Systems - physiology cryo-EM Cryoelectron Microscopy - methods Endonucleases - metabolism HEK293 Cells Humans Molecular Conformation Ribonucleases - metabolism Ribonucleases - physiology Ribonucleases - ultrastructure RNA - genetics RNA interference RNA targeting RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - physiology RNA, Guide, CRISPR-Cas Systems - ultrastructure shRNA structure type VI
Online-Zugang:	Volltext
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