Effects of Ginkgo biloba leaf extract, shenmai and matrine on a human embryonic lung fibroblast fibrosis model
The aim of the present study was to investigate the effects of leaf extract (GBLE), shenmai (S), and matrine (M) on human embryonic lung fibroblasts (HELFs). HELFs were allocated into the following groups: Group A (control group), group B [transforming growth factor β1 (TGF-β1) model group], groups...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2018-11, Vol.16 (5), p.4289-4295 |
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| creator | Zhang, Xingcai Cai, Yuli |
| description | The aim of the present study was to investigate the effects of
leaf extract (GBLE), shenmai (S), and matrine (M) on human embryonic lung fibroblasts (HELFs). HELFs were allocated into the following groups: Group A (control group), group B [transforming growth factor β1 (TGF-β1) model group], groups C1-3 (TGF-β1 + low-, moderate- and high-dose GBLE), groups D1-3 (TGF-β1 + low-, moderate- and high-dose S) and groups E1-3 (TGF-β1 + low-, moderate- and high-dose oM). Cell proliferation was assessed with an MTT assay and apoptosis was measured by annexin V/propidium iodide double staining and flow cytometry analysis. Collagen type I (COL-I), collagen type III (COL-III), α-smooth muscle actin (α-SMA) and extracellular superoxide dismutase (ECSOD) mRNA expression levels were measured using semi-quantitative reverse transcription-polymerase chain reaction, and protein content was measured using ELISA. The cell growth inhibition rates of the S groups were significantly higher than those of the other treatment groups (P |
| doi_str_mv | 10.3892/etm.2018.6698 |
| format | Article |
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leaf extract (GBLE), shenmai (S), and matrine (M) on human embryonic lung fibroblasts (HELFs). HELFs were allocated into the following groups: Group A (control group), group B [transforming growth factor β1 (TGF-β1) model group], groups C1-3 (TGF-β1 + low-, moderate- and high-dose GBLE), groups D1-3 (TGF-β1 + low-, moderate- and high-dose S) and groups E1-3 (TGF-β1 + low-, moderate- and high-dose oM). Cell proliferation was assessed with an MTT assay and apoptosis was measured by annexin V/propidium iodide double staining and flow cytometry analysis. Collagen type I (COL-I), collagen type III (COL-III), α-smooth muscle actin (α-SMA) and extracellular superoxide dismutase (ECSOD) mRNA expression levels were measured using semi-quantitative reverse transcription-polymerase chain reaction, and protein content was measured using ELISA. The cell growth inhibition rates of the S groups were significantly higher than those of the other treatment groups (P<0.05). The rate of apoptosis was significantly increased in the treatment groups compared with the model group (P<0.05), and S induced a significant increase in HELF apoptosis compared with the other treatment groups (P<0.05). The mRNA and protein expressions of COL-III, COL-I and α-SMA in the GBLE, S and M groups were significantly decreased, while the expression of ECSOD was significantly increased when compared with the model group (P<0.05). In conclusion, GBLE, S and M inhibited the pro-fibrotic role of TGF-β1 by targeting different steps in TGF-β1-mediated fibrosis.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2018.6698</identifier><identifier>PMID: 30344702</identifier><language>eng</language><publisher>Greece: Spandidos Publications UK Ltd</publisher><subject>Apoptosis ; Cell growth ; Collagen ; Drug dosages ; Extracellular matrix ; Fibroblasts ; Growth factors ; Hypotheses ; Inflammation ; Pathogenesis ; Pulmonary fibrosis ; Standard deviation ; Substance abuse treatment ; Ventilation</subject><ispartof>Experimental and therapeutic medicine, 2018-11, Vol.16 (5), p.4289-4295</ispartof><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright © 2018, Spandidos Publications 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-48ed327b3fdff0e9bd36d17867455d7f3146bb1118ddcaad4aa642e4bc543fc83</citedby><cites>FETCH-LOGICAL-c481t-48ed327b3fdff0e9bd36d17867455d7f3146bb1118ddcaad4aa642e4bc543fc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176143/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176143/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30344702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xingcai</creatorcontrib><creatorcontrib>Cai, Yuli</creatorcontrib><title>Effects of Ginkgo biloba leaf extract, shenmai and matrine on a human embryonic lung fibroblast fibrosis model</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>The aim of the present study was to investigate the effects of
leaf extract (GBLE), shenmai (S), and matrine (M) on human embryonic lung fibroblasts (HELFs). HELFs were allocated into the following groups: Group A (control group), group B [transforming growth factor β1 (TGF-β1) model group], groups C1-3 (TGF-β1 + low-, moderate- and high-dose GBLE), groups D1-3 (TGF-β1 + low-, moderate- and high-dose S) and groups E1-3 (TGF-β1 + low-, moderate- and high-dose oM). Cell proliferation was assessed with an MTT assay and apoptosis was measured by annexin V/propidium iodide double staining and flow cytometry analysis. Collagen type I (COL-I), collagen type III (COL-III), α-smooth muscle actin (α-SMA) and extracellular superoxide dismutase (ECSOD) mRNA expression levels were measured using semi-quantitative reverse transcription-polymerase chain reaction, and protein content was measured using ELISA. The cell growth inhibition rates of the S groups were significantly higher than those of the other treatment groups (P<0.05). The rate of apoptosis was significantly increased in the treatment groups compared with the model group (P<0.05), and S induced a significant increase in HELF apoptosis compared with the other treatment groups (P<0.05). The mRNA and protein expressions of COL-III, COL-I and α-SMA in the GBLE, S and M groups were significantly decreased, while the expression of ECSOD was significantly increased when compared with the model group (P<0.05). In conclusion, GBLE, S and M inhibited the pro-fibrotic role of TGF-β1 by targeting different steps in TGF-β1-mediated fibrosis.</description><subject>Apoptosis</subject><subject>Cell growth</subject><subject>Collagen</subject><subject>Drug dosages</subject><subject>Extracellular matrix</subject><subject>Fibroblasts</subject><subject>Growth factors</subject><subject>Hypotheses</subject><subject>Inflammation</subject><subject>Pathogenesis</subject><subject>Pulmonary fibrosis</subject><subject>Standard deviation</subject><subject>Substance abuse treatment</subject><subject>Ventilation</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkUtrVDEYhoMotoxdupWAGxeeMbeTk7MRpNQqFNzoOuQ6k5pLTc4p9t-bccaiZpMP8vDyvXkAeInRloqZvHNL2hKExZbzWTwB53iayYARHp-eZjQLfAYuWrtF_YwcCzE-B2cUUcYmRM5BvvLemaXB4uF1yN93BeoQi1YwOuWh-7lUZZa3sO1dTipAlS1MaqkhO1gyVHC_JpWhS7o-lBwMjGveQR90LTqqthzHFhpMxbr4AjzzKjZ3cbo34NvHq6-Xn4abL9efLz_cDIYJvAxMOEvJpKm33iM3a0u5xZPgExtHO3mKGdcaYyysNUpZphRnxDFtRka9EXQD3h9z71adnDUu9x5R3tWQVH2QRQX570sOe7kr95LjiWNGe8CbU0AtP1bXFplCMy5GlV1ZmySYjGQeBZs7-vo_9LasNfd6vynMGetbbcBwpEz_jladf1wGI3mQKbtMeZApDzI7_-rvBo_0H3X0F4JunHc</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Zhang, Xingcai</creator><creator>Cai, Yuli</creator><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20181101</creationdate><title>Effects of Ginkgo biloba leaf extract, shenmai and matrine on a human embryonic lung fibroblast fibrosis model</title><author>Zhang, Xingcai ; Cai, Yuli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-48ed327b3fdff0e9bd36d17867455d7f3146bb1118ddcaad4aa642e4bc543fc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apoptosis</topic><topic>Cell growth</topic><topic>Collagen</topic><topic>Drug dosages</topic><topic>Extracellular matrix</topic><topic>Fibroblasts</topic><topic>Growth factors</topic><topic>Hypotheses</topic><topic>Inflammation</topic><topic>Pathogenesis</topic><topic>Pulmonary fibrosis</topic><topic>Standard deviation</topic><topic>Substance abuse treatment</topic><topic>Ventilation</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xingcai</creatorcontrib><creatorcontrib>Cai, Yuli</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xingcai</au><au>Cai, Yuli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Ginkgo biloba leaf extract, shenmai and matrine on a human embryonic lung fibroblast fibrosis model</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>16</volume><issue>5</issue><spage>4289</spage><epage>4295</epage><pages>4289-4295</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>The aim of the present study was to investigate the effects of
leaf extract (GBLE), shenmai (S), and matrine (M) on human embryonic lung fibroblasts (HELFs). HELFs were allocated into the following groups: Group A (control group), group B [transforming growth factor β1 (TGF-β1) model group], groups C1-3 (TGF-β1 + low-, moderate- and high-dose GBLE), groups D1-3 (TGF-β1 + low-, moderate- and high-dose S) and groups E1-3 (TGF-β1 + low-, moderate- and high-dose oM). Cell proliferation was assessed with an MTT assay and apoptosis was measured by annexin V/propidium iodide double staining and flow cytometry analysis. Collagen type I (COL-I), collagen type III (COL-III), α-smooth muscle actin (α-SMA) and extracellular superoxide dismutase (ECSOD) mRNA expression levels were measured using semi-quantitative reverse transcription-polymerase chain reaction, and protein content was measured using ELISA. The cell growth inhibition rates of the S groups were significantly higher than those of the other treatment groups (P<0.05). The rate of apoptosis was significantly increased in the treatment groups compared with the model group (P<0.05), and S induced a significant increase in HELF apoptosis compared with the other treatment groups (P<0.05). The mRNA and protein expressions of COL-III, COL-I and α-SMA in the GBLE, S and M groups were significantly decreased, while the expression of ECSOD was significantly increased when compared with the model group (P<0.05). In conclusion, GBLE, S and M inhibited the pro-fibrotic role of TGF-β1 by targeting different steps in TGF-β1-mediated fibrosis.</abstract><cop>Greece</cop><pub>Spandidos Publications UK Ltd</pub><pmid>30344702</pmid><doi>10.3892/etm.2018.6698</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Cell growth Collagen Drug dosages Extracellular matrix Fibroblasts Growth factors Hypotheses Inflammation Pathogenesis Pulmonary fibrosis Standard deviation Substance abuse treatment Ventilation |
| title | Effects of Ginkgo biloba leaf extract, shenmai and matrine on a human embryonic lung fibroblast fibrosis model |
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