Phylogenetics of Mycoplasma hominis clinical strains associated with gynecological infections or infertility as disclosed by an expanded multilocus sequence typing scheme
To our knowledge, the phylodistribution of M. hominis clinical strains associated with various pathological conditions of the urogenital tract has not been explored hitherto. Here we analyzed the genetic diversity and phylogenetic relationships among 59 M. hominis Tunisian clinical isolates, catego...
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| Veröffentlicht in: | Scientific reports 2018-10, Vol.8 (1), p.14854-10, Article 14854 |
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| description | To our knowledge, the phylodistribution of
M. hominis
clinical strains associated with various pathological conditions of the urogenital tract has not been explored hitherto. Here we analyzed the genetic diversity and phylogenetic relationships among 59
M. hominis
Tunisian clinical isolates, categorized as gynecological infections- or infertility-associated pathotypes. For this purpose, we developed an expanded multilocus sequence typing (eMLST) scheme, combining the previously reported multilocus sequence typing (MLST) loci (
gyrB, tuf, ftsY, uvrA, gap
) with a new selected set of putative virulence genes (
p120’, vaa, lmp1, lmp3, p60
), referred herein to as multi-virulence-locus sequence typing (MVLST) loci. In doing so,
M. hominis
population was segregated into two distinct genetic lineages, which were differentially associated with each pathotype. Such a clear dichotomy was supported by several phylogenetic and population genetic analysis tools. Recombination was found to take place, but not sufficient enough to break down the overall clonal population structure of
M. hominis
, most likely as a result of purifying selection, which accommodated the most fit clones. In sum, and owing to the eMLST scheme described herein, we provide insightful data on the phylogenetics of
M. hominis
, arguing for the existence of genetically differentiable urogenital pathotypes. |
| doi_str_mv | 10.1038/s41598-018-33260-x |
| format | Article |
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M. hominis
clinical strains associated with various pathological conditions of the urogenital tract has not been explored hitherto. Here we analyzed the genetic diversity and phylogenetic relationships among 59
M. hominis
Tunisian clinical isolates, categorized as gynecological infections- or infertility-associated pathotypes. For this purpose, we developed an expanded multilocus sequence typing (eMLST) scheme, combining the previously reported multilocus sequence typing (MLST) loci (
gyrB, tuf, ftsY, uvrA, gap
) with a new selected set of putative virulence genes (
p120’, vaa, lmp1, lmp3, p60
), referred herein to as multi-virulence-locus sequence typing (MVLST) loci. In doing so,
M. hominis
population was segregated into two distinct genetic lineages, which were differentially associated with each pathotype. Such a clear dichotomy was supported by several phylogenetic and population genetic analysis tools. Recombination was found to take place, but not sufficient enough to break down the overall clonal population structure of
M. hominis
, most likely as a result of purifying selection, which accommodated the most fit clones. In sum, and owing to the eMLST scheme described herein, we provide insightful data on the phylogenetics of
M. hominis
, arguing for the existence of genetically differentiable urogenital pathotypes.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-33260-x</identifier><identifier>PMID: 30291332</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>45/23 ; 45/77 ; 631/326/2521 ; 631/326/325/2482 ; Clinical isolates ; Genetic analysis ; Genetic diversity ; Gynecology ; Humanities and Social Sciences ; Infertility ; Life Sciences ; multidisciplinary ; Multilocus sequence typing ; Phylogenetics ; Phylogeny ; Population genetics ; Population structure ; Recombination ; Science ; Science (multidisciplinary) ; Virulence</subject><ispartof>Scientific reports, 2018-10, Vol.8 (1), p.14854-10, Article 14854</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-da17f07f0eaa4b4a430720118b7dafc6e1b823374bfb75817bbd17a092bb937f3</citedby><cites>FETCH-LOGICAL-c565t-da17f07f0eaa4b4a430720118b7dafc6e1b823374bfb75817bbd17a092bb937f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173709/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173709/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,42188,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30291332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://riip.hal.science/pasteur-01990488$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Boujemaa, Safa</creatorcontrib><creatorcontrib>Ben Allaya, Amina</creatorcontrib><creatorcontrib>Mlik, Béhija</creatorcontrib><creatorcontrib>Mardassi, Helmi</creatorcontrib><creatorcontrib>Ben Abdelmoumen Mardassi, Boutheina</creatorcontrib><title>Phylogenetics of Mycoplasma hominis clinical strains associated with gynecological infections or infertility as disclosed by an expanded multilocus sequence typing scheme</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>To our knowledge, the phylodistribution of
M. hominis
clinical strains associated with various pathological conditions of the urogenital tract has not been explored hitherto. Here we analyzed the genetic diversity and phylogenetic relationships among 59
M. hominis
Tunisian clinical isolates, categorized as gynecological infections- or infertility-associated pathotypes. For this purpose, we developed an expanded multilocus sequence typing (eMLST) scheme, combining the previously reported multilocus sequence typing (MLST) loci (
gyrB, tuf, ftsY, uvrA, gap
) with a new selected set of putative virulence genes (
p120’, vaa, lmp1, lmp3, p60
), referred herein to as multi-virulence-locus sequence typing (MVLST) loci. In doing so,
M. hominis
population was segregated into two distinct genetic lineages, which were differentially associated with each pathotype. Such a clear dichotomy was supported by several phylogenetic and population genetic analysis tools. Recombination was found to take place, but not sufficient enough to break down the overall clonal population structure of
M. hominis
, most likely as a result of purifying selection, which accommodated the most fit clones. In sum, and owing to the eMLST scheme described herein, we provide insightful data on the phylogenetics of
M. hominis
, arguing for the existence of genetically differentiable urogenital pathotypes.</description><subject>45/23</subject><subject>45/77</subject><subject>631/326/2521</subject><subject>631/326/325/2482</subject><subject>Clinical isolates</subject><subject>Genetic analysis</subject><subject>Genetic diversity</subject><subject>Gynecology</subject><subject>Humanities and Social Sciences</subject><subject>Infertility</subject><subject>Life Sciences</subject><subject>multidisciplinary</subject><subject>Multilocus sequence typing</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Population genetics</subject><subject>Population structure</subject><subject>Recombination</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Virulence</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kstu1TAQhiMEolXpC7BAltiwCfiSmzdIVQUU6SBYwNqyHefElWMH2yknr8RTMpyUUrrAsmSP_P3_zFhTFM8Jfk0w696kitS8KzHpSsZog8vDo-KU4qouKaP08b37SXGe0jWGVVNeEf60OGGYcgKy0-Lnl3F1YW-8yVYnFAb0adVhdjJNEo1hst4mpB0cWjqUcpTWJyRTCtrKbHr0w-YR7VdvdACfI2X9YHS2AcAQj1HM1tm8gg71NmkXEigVxB6Zwyx9D-G0OKCCXhJK5vtivDYor7P1e5T0aCbzrHgySJfM-e15Vnx7_-7r5VW5-_zh4-XFrtR1U-eyl6QdMGwjZaUqWTHcUkxIp9peDroxRHWUsbZSg2rrjrRK9aSVmFOlOGsHdla83XznRU2m18ZD107M0U4yriJIK_598XYU-3AjGtKyFnMwKDeD8YHs6mInZpmyWaLAhHNcdd0NAf7VbcIYoPGUxQSfZJyT3oQlCUpI01Uc1zWgLx-g12GJHr7jSDWYE94ARTdKx5BSNMNdFQSL39MjtumBIjpxnB5xANGL-23fSf7MCgBsAxI8-b2Jf3P_x_YXxgfWPQ</recordid><startdate>20181005</startdate><enddate>20181005</enddate><creator>Boujemaa, Safa</creator><creator>Ben Allaya, Amina</creator><creator>Mlik, Béhija</creator><creator>Mardassi, Helmi</creator><creator>Ben Abdelmoumen Mardassi, Boutheina</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope></search><sort><creationdate>20181005</creationdate><title>Phylogenetics of Mycoplasma hominis clinical strains associated with gynecological infections or infertility as disclosed by an expanded multilocus sequence typing scheme</title><author>Boujemaa, Safa ; 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M. hominis
clinical strains associated with various pathological conditions of the urogenital tract has not been explored hitherto. Here we analyzed the genetic diversity and phylogenetic relationships among 59
M. hominis
Tunisian clinical isolates, categorized as gynecological infections- or infertility-associated pathotypes. For this purpose, we developed an expanded multilocus sequence typing (eMLST) scheme, combining the previously reported multilocus sequence typing (MLST) loci (
gyrB, tuf, ftsY, uvrA, gap
) with a new selected set of putative virulence genes (
p120’, vaa, lmp1, lmp3, p60
), referred herein to as multi-virulence-locus sequence typing (MVLST) loci. In doing so,
M. hominis
population was segregated into two distinct genetic lineages, which were differentially associated with each pathotype. Such a clear dichotomy was supported by several phylogenetic and population genetic analysis tools. Recombination was found to take place, but not sufficient enough to break down the overall clonal population structure of
M. hominis
, most likely as a result of purifying selection, which accommodated the most fit clones. In sum, and owing to the eMLST scheme described herein, we provide insightful data on the phylogenetics of
M. hominis
, arguing for the existence of genetically differentiable urogenital pathotypes.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30291332</pmid><doi>10.1038/s41598-018-33260-x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 45/23 45/77 631/326/2521 631/326/325/2482 Clinical isolates Genetic analysis Genetic diversity Gynecology Humanities and Social Sciences Infertility Life Sciences multidisciplinary Multilocus sequence typing Phylogenetics Phylogeny Population genetics Population structure Recombination Science Science (multidisciplinary) Virulence |
| title | Phylogenetics of Mycoplasma hominis clinical strains associated with gynecological infections or infertility as disclosed by an expanded multilocus sequence typing scheme |
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