Development of a Quantitative PCR Assay for Arcobacter spp. and its Application to Environmental Water Samples

Arcobacter spp. are emerging pathogens associated with gastroenteritis in humans. The objective of this study was to develop a highly sensitive and broadly reactive quantitative PCR (qPCR) assay for Arcobacter spp. and to apply the developed assay to different water sources in the Kathmandu Valley,...

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Veröffentlicht in:	Microbes and Environments 2018, Vol.33(3), pp.309-316
Hauptverfasser:	Shrestha, Rajani Ghaju, Tanaka, Yasuhiro, Malla, Bikash, Tandukar, Sarmila, Bhandari, Dinesh, Inoue, Daisuke, Sei, Kazunari, Sherchand, Jeevan B., Haramoto, Eiji
Format:	Artikel
Sprache:	eng
Schlagworte:	Arcobacter Arcobacter - genetics Arcobacter spp Assaying Base Sequence Deoxyribonucleic acid DNA DNA, Bacterial - genetics Dug wells Environmental Monitoring - methods Gastroenteritis Gene sequencing High-Throughput Nucleotide Sequencing Homology Japan Kathmandu Valley Next-generation sequencing Nucleotide sequence Nucleotides Pathogens PCR Polymerase chain reaction Primers Protozoa quantitative PCR Real-Time Polymerase Chain Reaction Rivers RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA Species Specificity Water analysis Water Microbiology Water sampling water source Water springs Water wells
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