Paramagnetic Tag for Glycosylation Sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1

An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied. The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins b...

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Veröffentlicht in:	ACS chemical biology 2018-09, Vol.13 (9), p.2560-2567
Hauptverfasser:	Moure, Maria J, Eletsky, Alexander, Gao, Qi, Morris, Laura C, Yang, Jeong-Yeh, Chapla, Digantkumar, Zhao, Yuejie, Zong, Chengli, Amster, I. Jonathan, Moremen, Kelley W, Boons, Geert-Jan, Prestegard, James H
Format:	Artikel
Sprache:	eng
Schlagworte:	Click Chemistry Cyclic N-Oxides - analysis Cyclic N-Oxides - metabolism Electron Spin Resonance Spectroscopy Glycoproteins - chemistry Glycoproteins - metabolism Glycosylation HEK293 Cells Heparitin Sulfate - chemistry Heparitin Sulfate - metabolism Humans Ligands Molecular Docking Simulation Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - metabolism Nuclear Magnetic Resonance, Biomolecular Protein Binding Receptors, Immunologic - chemistry Receptors, Immunologic - metabolism Roundabout Proteins
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