Automated measurement of upper thermal limits in small aquatic animals
We present a method for automating the measurement of upper thermal limits in small aquatic organisms. Upper thermal limits are frequently defined by the cessation of movement at high temperature, with measurement being performed by manual observation. Consequently, estimates of upper thermal limits...
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| Veröffentlicht in: | Journal of experimental biology 2018-09, Vol.221 (Pt 17) |
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| container_title | Journal of experimental biology |
| container_volume | 221 |
| creator | Burton, Tim Zeis, Bettina Einum, Sigurd |
| description | We present a method for automating the measurement of upper thermal limits in small aquatic organisms. Upper thermal limits are frequently defined by the cessation of movement at high temperature, with measurement being performed by manual observation. Consequently, estimates of upper thermal limits may be subject to error and bias, both within and among observers. Our method utilises video-based tracking software to record the movement of individuals when exposed to high, lethal temperatures. We develop an algorithm in the R computing language that can objectively identify the loss of locomotory function from tracking data. Using independent experimental data, we validate our approach by demonstrating the expected response in upper thermal limits to acclimation temperature. |
| doi_str_mv | 10.1242/jeb.182386 |
| format | Article |
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Upper thermal limits are frequently defined by the cessation of movement at high temperature, with measurement being performed by manual observation. Consequently, estimates of upper thermal limits may be subject to error and bias, both within and among observers. Our method utilises video-based tracking software to record the movement of individuals when exposed to high, lethal temperatures. We develop an algorithm in the R computing language that can objectively identify the loss of locomotory function from tracking data. Using independent experimental data, we validate our approach by demonstrating the expected response in upper thermal limits to acclimation temperature.</description><identifier>ISSN: 0022-0949</identifier><identifier>EISSN: 1477-9145</identifier><identifier>DOI: 10.1242/jeb.182386</identifier><identifier>PMID: 30012577</identifier><language>eng</language><publisher>England: The Company of Biologists Ltd</publisher><subject>Acclimatization - physiology ; Animals ; Aquatic Organisms - physiology ; Daphnia - physiology ; Female ; Hot Temperature ; Hydrobiology - methods ; Methods and Techniques ; Physiology - methods ; Zoology - methods</subject><ispartof>Journal of experimental biology, 2018-09, Vol.221 (Pt 17)</ispartof><rights>2018. Published by The Company of Biologists Ltd.</rights><rights>2018. 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Upper thermal limits are frequently defined by the cessation of movement at high temperature, with measurement being performed by manual observation. Consequently, estimates of upper thermal limits may be subject to error and bias, both within and among observers. Our method utilises video-based tracking software to record the movement of individuals when exposed to high, lethal temperatures. We develop an algorithm in the R computing language that can objectively identify the loss of locomotory function from tracking data. Using independent experimental data, we validate our approach by demonstrating the expected response in upper thermal limits to acclimation temperature.</description><subject>Acclimatization - physiology</subject><subject>Animals</subject><subject>Aquatic Organisms - physiology</subject><subject>Daphnia - physiology</subject><subject>Female</subject><subject>Hot Temperature</subject><subject>Hydrobiology - methods</subject><subject>Methods and Techniques</subject><subject>Physiology - methods</subject><subject>Zoology - methods</subject><issn>0022-0949</issn><issn>1477-9145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUEtLAzEYDKLYWr34AyRHEbbmtUn2IpRiVSh46T1ks4lN2VeTXcF_b6S16HcZhhnmGwaAW4zmmDDyuLPlHEtCJT8DU8yEyArM8nMwRYiQDBWsmICrGHcoHc_ZJZhQhDDJhZiC1WIcukYPtoKN1XEMtrHtADsHx763AQ5bGxpdw9o3fojQtzAmWkO9H_XgDdStTzxegwuXwN4ccQY2q-fN8jVbv7-8LRfrzFAhh8zwSriKOqdLxohmVBBTUsSFFMQZzSWTnDhCOa2wNIWshCm1zJ1LqnSSzsDTIbYfy8ZWJlUNulZ9SCXCl-q0V_-V1m_VR_epOGaIYpoC7o8BoduPNg6q8dHYutat7caoCBI45zliJFkfDlYTuhiDdac3GKmf3VXaXR12T-a7v8VO1t-h6TdA43_f</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Burton, Tim</creator><creator>Zeis, Bettina</creator><creator>Einum, Sigurd</creator><general>The Company of Biologists Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0215-0227</orcidid></search><sort><creationdate>20180901</creationdate><title>Automated measurement of upper thermal limits in small aquatic animals</title><author>Burton, Tim ; Zeis, Bettina ; Einum, Sigurd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-c6d7fd3ffab442a4372cb3067872fca684862f2363d18c98d7cba85ff2fc8f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acclimatization - physiology</topic><topic>Animals</topic><topic>Aquatic Organisms - physiology</topic><topic>Daphnia - physiology</topic><topic>Female</topic><topic>Hot Temperature</topic><topic>Hydrobiology - methods</topic><topic>Methods and Techniques</topic><topic>Physiology - methods</topic><topic>Zoology - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burton, Tim</creatorcontrib><creatorcontrib>Zeis, Bettina</creatorcontrib><creatorcontrib>Einum, Sigurd</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of experimental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burton, Tim</au><au>Zeis, Bettina</au><au>Einum, Sigurd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automated measurement of upper thermal limits in small aquatic animals</atitle><jtitle>Journal of experimental biology</jtitle><addtitle>J Exp Biol</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>221</volume><issue>Pt 17</issue><issn>0022-0949</issn><eissn>1477-9145</eissn><abstract>We present a method for automating the measurement of upper thermal limits in small aquatic organisms. Upper thermal limits are frequently defined by the cessation of movement at high temperature, with measurement being performed by manual observation. Consequently, estimates of upper thermal limits may be subject to error and bias, both within and among observers. Our method utilises video-based tracking software to record the movement of individuals when exposed to high, lethal temperatures. We develop an algorithm in the R computing language that can objectively identify the loss of locomotory function from tracking data. Using independent experimental data, we validate our approach by demonstrating the expected response in upper thermal limits to acclimation temperature.</abstract><cop>England</cop><pub>The Company of Biologists Ltd</pub><pmid>30012577</pmid><doi>10.1242/jeb.182386</doi><orcidid>https://orcid.org/0000-0002-0215-0227</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of experimental biology, 2018-09, Vol.221 (Pt 17) |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Acclimatization - physiology Animals Aquatic Organisms - physiology Daphnia - physiology Female Hot Temperature Hydrobiology - methods Methods and Techniques Physiology - methods Zoology - methods |
| title | Automated measurement of upper thermal limits in small aquatic animals |
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