Regulation of blood‐testis barrier assembly in vivo by germ cells
The assembly of the blood‐testis barrier (BTB) during postnatal development is crucial to support meiosis. However, the role of germ cells in BTB assembly remains unclear. Herein, KitW/KitWv mice were used as a study model. These mice were infertile, failing to establish a functional BTB to support...
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| creator | Li, Xiao‐Yu Zhang, Yan Wang, Xiu‐Xia Jin, Cheng Wang, Yu‐Qian Sun, Tie‐Cheng Li, Jian Tang, Ji‐Xin Batool, Alia Deng, Shou‐Long Chen, Su‐Ren Yan Cheng, C. Liu, Yi‐Xun |
| description | The assembly of the blood‐testis barrier (BTB) during postnatal development is crucial to support meiosis. However, the role of germ cells in BTB assembly remains unclear. Herein, KitW/KitWv mice were used as a study model. These mice were infertile, failing to establish a functional BTB to support meiosis due to c‐Kit mutation. Transplantation of undifferentiated spermatogonia derived from normal mice into the testis of KitW/ KitWV mice triggered functional BTB assembly, displaying cyclic remodeling during the epithelial cycle. Also, transplanted germ cells were capable of inducing Leydig cell testosterone production, which could enhance the expression of integral membrane protein claudin 3 in Sertoli cells. Early spermatocytes were shown to play a vital role in directing BTB assembly by expressing claudin 3, which likely created a transient adhesion structure to mediate BTB and cytoskeleton assembly in adjacent Sertoli cells. In summary, the positive modulation of germ cells on somatic cell function provides useful information regarding somatic‐germ cell interactions.—Li, X.‐Y., Zhang Y., Wang, X.‐X., Jin, C., Wang Y.‐Q., Sun, T.‐C., Li, J., Tang J.‐X., Batool, A., Deng, S.‐L., Chen S.‐R., Cheng, C. Y., Liu, Y.‐X. Regulation of blood‐testis barrier assembly in vivo by germ cells. FASEB J. 32, 1653‐1664 (2018). www.fasebj.org |
| doi_str_mv | 10.1096/fj.201700681R |
| format | Article |
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However, the role of germ cells in BTB assembly remains unclear. Herein, KitW/KitWv mice were used as a study model. These mice were infertile, failing to establish a functional BTB to support meiosis due to c‐Kit mutation. Transplantation of undifferentiated spermatogonia derived from normal mice into the testis of KitW/ KitWV mice triggered functional BTB assembly, displaying cyclic remodeling during the epithelial cycle. Also, transplanted germ cells were capable of inducing Leydig cell testosterone production, which could enhance the expression of integral membrane protein claudin 3 in Sertoli cells. Early spermatocytes were shown to play a vital role in directing BTB assembly by expressing claudin 3, which likely created a transient adhesion structure to mediate BTB and cytoskeleton assembly in adjacent Sertoli cells. In summary, the positive modulation of germ cells on somatic cell function provides useful information regarding somatic‐germ cell interactions.—Li, X.‐Y., Zhang Y., Wang, X.‐X., Jin, C., Wang Y.‐Q., Sun, T.‐C., Li, J., Tang J.‐X., Batool, A., Deng, S.‐L., Chen S.‐R., Cheng, C. Y., Liu, Y.‐X. Regulation of blood‐testis barrier assembly in vivo by germ cells. FASEB J. 32, 1653‐1664 (2018). www.fasebj.org</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.201700681R</identifier><identifier>PMID: 29183964</identifier><language>eng</language><publisher>United States: Federation of American Societies for Experimental Biology</publisher><subject>Animals ; Blood-Testis Barrier - cytology ; Blood-Testis Barrier - metabolism ; claudin 3 ; Claudin-3 - biosynthesis ; Claudin-3 - genetics ; Leydig Cells - cytology ; Leydig Cells - metabolism ; Male ; Mice ; Mice, Transgenic ; Sertoli Cells - cytology ; Sertoli Cells - metabolism ; Spermatogonia - cytology ; Spermatogonia - metabolism ; testosterone ; tight junction</subject><ispartof>The FASEB journal, 2018-03, Vol.32 (3), p.1653-1664</ispartof><rights>FASEB</rights><rights>FASEB 2018 FASEB</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439R-1b9928015486d15e4ed8299d562e9b21eabdbff791e718ed34aac643594e4ba93</citedby><cites>FETCH-LOGICAL-c439R-1b9928015486d15e4ed8299d562e9b21eabdbff791e718ed34aac643594e4ba93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.201700681R$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.201700681R$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29183964$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiao‐Yu</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Wang, Xiu‐Xia</creatorcontrib><creatorcontrib>Jin, Cheng</creatorcontrib><creatorcontrib>Wang, Yu‐Qian</creatorcontrib><creatorcontrib>Sun, Tie‐Cheng</creatorcontrib><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>Tang, Ji‐Xin</creatorcontrib><creatorcontrib>Batool, Alia</creatorcontrib><creatorcontrib>Deng, Shou‐Long</creatorcontrib><creatorcontrib>Chen, Su‐Ren</creatorcontrib><creatorcontrib>Yan Cheng, C.</creatorcontrib><creatorcontrib>Liu, Yi‐Xun</creatorcontrib><title>Regulation of blood‐testis barrier assembly in vivo by germ cells</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>The assembly of the blood‐testis barrier (BTB) during postnatal development is crucial to support meiosis. However, the role of germ cells in BTB assembly remains unclear. Herein, KitW/KitWv mice were used as a study model. These mice were infertile, failing to establish a functional BTB to support meiosis due to c‐Kit mutation. Transplantation of undifferentiated spermatogonia derived from normal mice into the testis of KitW/ KitWV mice triggered functional BTB assembly, displaying cyclic remodeling during the epithelial cycle. Also, transplanted germ cells were capable of inducing Leydig cell testosterone production, which could enhance the expression of integral membrane protein claudin 3 in Sertoli cells. Early spermatocytes were shown to play a vital role in directing BTB assembly by expressing claudin 3, which likely created a transient adhesion structure to mediate BTB and cytoskeleton assembly in adjacent Sertoli cells. In summary, the positive modulation of germ cells on somatic cell function provides useful information regarding somatic‐germ cell interactions.—Li, X.‐Y., Zhang Y., Wang, X.‐X., Jin, C., Wang Y.‐Q., Sun, T.‐C., Li, J., Tang J.‐X., Batool, A., Deng, S.‐L., Chen S.‐R., Cheng, C. Y., Liu, Y.‐X. Regulation of blood‐testis barrier assembly in vivo by germ cells. FASEB J. 32, 1653‐1664 (2018). www.fasebj.org</description><subject>Animals</subject><subject>Blood-Testis Barrier - cytology</subject><subject>Blood-Testis Barrier - metabolism</subject><subject>claudin 3</subject><subject>Claudin-3 - biosynthesis</subject><subject>Claudin-3 - genetics</subject><subject>Leydig Cells - cytology</subject><subject>Leydig Cells - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Sertoli Cells - cytology</subject><subject>Sertoli Cells - metabolism</subject><subject>Spermatogonia - cytology</subject><subject>Spermatogonia - metabolism</subject><subject>testosterone</subject><subject>tight junction</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PGzEQhq2qqKTAsdfKx14WPLbXa6tSpRI1BYRUKYWzZe_Opo5219ROUuXWn9DfyC9hUfjqhdMc5tEz876EfAB2DMyok3Z5zBlUjCkN8zdkAqVghdKKvSUTpg0vlBJ6n7zPeckYAwbqHdnnBrQwSk7IdI6LdedWIQ40ttR3MTa3f_-tMK9Cpt6lFDBRlzP2vtvSMNBN2ETqt3SBqac1dl0-JHut6zIePcwDcj37djU9Ky5_fD-ffr0sainMvABvDNcMSqlVAyVKbDQ3pikVR-M5oPONb9vKAFagsRHSuVpJURqJ0jsjDsiXnfdm7XtsahxWyXX2JoXepa2NLtj_N0P4ZRdxYxWISpZsFHx6EKT4ez1GtH3I9xHcgHGdLZiK8QqUFiNa7NA6xZwTtk9ngNn74m27tM_Fj_zHl7890Y9Nj8DnHfAndLh93WZnP0_57OKF_g4KRZGb</recordid><startdate>201803</startdate><enddate>201803</enddate><creator>Li, Xiao‐Yu</creator><creator>Zhang, Yan</creator><creator>Wang, Xiu‐Xia</creator><creator>Jin, Cheng</creator><creator>Wang, Yu‐Qian</creator><creator>Sun, Tie‐Cheng</creator><creator>Li, Jian</creator><creator>Tang, Ji‐Xin</creator><creator>Batool, Alia</creator><creator>Deng, Shou‐Long</creator><creator>Chen, Su‐Ren</creator><creator>Yan Cheng, C.</creator><creator>Liu, Yi‐Xun</creator><general>Federation of American Societies for Experimental Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201803</creationdate><title>Regulation of blood‐testis barrier assembly in vivo by germ cells</title><author>Li, Xiao‐Yu ; Zhang, Yan ; Wang, Xiu‐Xia ; Jin, Cheng ; Wang, Yu‐Qian ; Sun, Tie‐Cheng ; Li, Jian ; Tang, Ji‐Xin ; Batool, Alia ; Deng, Shou‐Long ; Chen, Su‐Ren ; Yan Cheng, C. ; Liu, Yi‐Xun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439R-1b9928015486d15e4ed8299d562e9b21eabdbff791e718ed34aac643594e4ba93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Blood-Testis Barrier - cytology</topic><topic>Blood-Testis Barrier - metabolism</topic><topic>claudin 3</topic><topic>Claudin-3 - biosynthesis</topic><topic>Claudin-3 - genetics</topic><topic>Leydig Cells - cytology</topic><topic>Leydig Cells - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Sertoli Cells - cytology</topic><topic>Sertoli Cells - metabolism</topic><topic>Spermatogonia - cytology</topic><topic>Spermatogonia - metabolism</topic><topic>testosterone</topic><topic>tight junction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiao‐Yu</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Wang, Xiu‐Xia</creatorcontrib><creatorcontrib>Jin, Cheng</creatorcontrib><creatorcontrib>Wang, Yu‐Qian</creatorcontrib><creatorcontrib>Sun, Tie‐Cheng</creatorcontrib><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>Tang, Ji‐Xin</creatorcontrib><creatorcontrib>Batool, Alia</creatorcontrib><creatorcontrib>Deng, Shou‐Long</creatorcontrib><creatorcontrib>Chen, Su‐Ren</creatorcontrib><creatorcontrib>Yan Cheng, C.</creatorcontrib><creatorcontrib>Liu, Yi‐Xun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiao‐Yu</au><au>Zhang, Yan</au><au>Wang, Xiu‐Xia</au><au>Jin, Cheng</au><au>Wang, Yu‐Qian</au><au>Sun, Tie‐Cheng</au><au>Li, Jian</au><au>Tang, Ji‐Xin</au><au>Batool, Alia</au><au>Deng, Shou‐Long</au><au>Chen, Su‐Ren</au><au>Yan Cheng, C.</au><au>Liu, Yi‐Xun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of blood‐testis barrier assembly in vivo by germ cells</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2018-03</date><risdate>2018</risdate><volume>32</volume><issue>3</issue><spage>1653</spage><epage>1664</epage><pages>1653-1664</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>The assembly of the blood‐testis barrier (BTB) during postnatal development is crucial to support meiosis. However, the role of germ cells in BTB assembly remains unclear. Herein, KitW/KitWv mice were used as a study model. These mice were infertile, failing to establish a functional BTB to support meiosis due to c‐Kit mutation. Transplantation of undifferentiated spermatogonia derived from normal mice into the testis of KitW/ KitWV mice triggered functional BTB assembly, displaying cyclic remodeling during the epithelial cycle. Also, transplanted germ cells were capable of inducing Leydig cell testosterone production, which could enhance the expression of integral membrane protein claudin 3 in Sertoli cells. Early spermatocytes were shown to play a vital role in directing BTB assembly by expressing claudin 3, which likely created a transient adhesion structure to mediate BTB and cytoskeleton assembly in adjacent Sertoli cells. In summary, the positive modulation of germ cells on somatic cell function provides useful information regarding somatic‐germ cell interactions.—Li, X.‐Y., Zhang Y., Wang, X.‐X., Jin, C., Wang Y.‐Q., Sun, T.‐C., Li, J., Tang J.‐X., Batool, A., Deng, S.‐L., Chen S.‐R., Cheng, C. Y., Liu, Y.‐X. Regulation of blood‐testis barrier assembly in vivo by germ cells. FASEB J. 32, 1653‐1664 (2018). www.fasebj.org</abstract><cop>United States</cop><pub>Federation of American Societies for Experimental Biology</pub><pmid>29183964</pmid><doi>10.1096/fj.201700681R</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Blood-Testis Barrier - cytology Blood-Testis Barrier - metabolism claudin 3 Claudin-3 - biosynthesis Claudin-3 - genetics Leydig Cells - cytology Leydig Cells - metabolism Male Mice Mice, Transgenic Sertoli Cells - cytology Sertoli Cells - metabolism Spermatogonia - cytology Spermatogonia - metabolism testosterone tight junction |
| title | Regulation of blood‐testis barrier assembly in vivo by germ cells |
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