Integrated Phloem Sap mRNA and Protein Expression Analysis Reveals Phytoplasma-infection Responses in Mulberry
To gain insight into the response of mulberry to phytoplasma-infection, the expression profiles of mRNAs and proteins in mulberry phloem sap were examined. A total of 955 unigenes and 136 proteins were found to be differentially expressed between the healthy and infected phloem sap. These differenti...
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| Veröffentlicht in: | Molecular & cellular proteomics 2018-09, Vol.17 (9), p.1702-1719 |
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| creator | Gai, Ying-Ping Yuan, Shuo-Shuo Liu, Zhao-Yang Zhao, Huai-Ning Liu, Qi Qin, Rong-Li Fang, Li-Jing Ji, Xian-Ling |
| description | To gain insight into the response of mulberry to phytoplasma-infection, the expression profiles of mRNAs and proteins in mulberry phloem sap were examined. A total of 955 unigenes and 136 proteins were found to be differentially expressed between the healthy and infected phloem sap. These differentially expressed mRNAs and proteins are involved in signaling, hormone metabolism, stress responses, etc. Interestingly, we found that both the mRNA and protein levels of the major latex protein-like 329 (MuMLPL329) gene were increased in the infected phloem saps. Expression of the MuMLPL329 gene was induced by pathogen inoculation and was responsive to jasmonic acid. Ectopic expression of MuMLPL329 in Arabidopsis enhances transgenic plant resistance to Botrytis cinerea, Pseudomonas syringae pv tomato DC3000 (Pst. DC3000) and phytoplasma. Further analysis revealed that MuMLPL329 can enhance the expression of some defense genes and might be involved in altering flavonoid content resulting in increased resistance of plants to pathogen infection. Finally, the roles of the differentially expressed mRNAs and proteins and the potential molecular mechanisms of their changes were discussed. It was likely that the phytoplasma-responsive mRNAs and proteins in the phloem saps were involved in multiple pathways of mulberry responses to phytoplasma-infection, and their changes may be partially responsible for some symptoms in the phytoplasma infected plants. |
| doi_str_mv | 10.1074/mcp.RA118.000670 |
| format | Article |
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A total of 955 unigenes and 136 proteins were found to be differentially expressed between the healthy and infected phloem sap. These differentially expressed mRNAs and proteins are involved in signaling, hormone metabolism, stress responses, etc. Interestingly, we found that both the mRNA and protein levels of the major latex protein-like 329 (MuMLPL329) gene were increased in the infected phloem saps. Expression of the MuMLPL329 gene was induced by pathogen inoculation and was responsive to jasmonic acid. Ectopic expression of MuMLPL329 in Arabidopsis enhances transgenic plant resistance to Botrytis cinerea, Pseudomonas syringae pv tomato DC3000 (Pst. DC3000) and phytoplasma. Further analysis revealed that MuMLPL329 can enhance the expression of some defense genes and might be involved in altering flavonoid content resulting in increased resistance of plants to pathogen infection. Finally, the roles of the differentially expressed mRNAs and proteins and the potential molecular mechanisms of their changes were discussed. It was likely that the phytoplasma-responsive mRNAs and proteins in the phloem saps were involved in multiple pathways of mulberry responses to phytoplasma-infection, and their changes may be partially responsible for some symptoms in the phytoplasma infected plants.</description><identifier>ISSN: 1535-9476</identifier><identifier>EISSN: 1535-9484</identifier><identifier>DOI: 10.1074/mcp.RA118.000670</identifier><identifier>PMID: 29848783</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Arabidopsis - genetics ; Arabidopsis - microbiology ; Down-Regulation - genetics ; Flavonoids - analysis ; Gene Expression ; Gene Expression Regulation, Plant ; Gene Ontology ; Genes, Plant ; Host-Pathogen Interaction ; iTRAQ ; Molecular biology ; Morus - genetics ; Morus - metabolism ; Morus - microbiology ; mulberry ; Nicotiana - genetics ; Phenotype ; Phloem - metabolism ; Phloem - microbiology ; phloem sap ; Phylogeny ; phytoplasm ; Phytoplasma - physiology ; Plant Diseases - microbiology ; Plant Leaves - metabolism ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified ; proteome ; Pseudomonas syringae - physiology ; Ribulose-Bisphosphate Carboxylase - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Stress response ; transcriptome ; Up-Regulation - genetics</subject><ispartof>Molecular & cellular proteomics, 2018-09, Vol.17 (9), p.1702-1719</ispartof><rights>2018 © 2018 Gai et al.</rights><rights>2018 Gai et al.</rights><rights>2018 Gai et al. 2018 Gai et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-8d4cf5d1098cd8b3799a967e8e444917bcabfc5e113440d6b53982cbf1e29baa3</citedby><cites>FETCH-LOGICAL-c447t-8d4cf5d1098cd8b3799a967e8e444917bcabfc5e113440d6b53982cbf1e29baa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126391/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126391/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29848783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gai, Ying-Ping</creatorcontrib><creatorcontrib>Yuan, Shuo-Shuo</creatorcontrib><creatorcontrib>Liu, Zhao-Yang</creatorcontrib><creatorcontrib>Zhao, Huai-Ning</creatorcontrib><creatorcontrib>Liu, Qi</creatorcontrib><creatorcontrib>Qin, Rong-Li</creatorcontrib><creatorcontrib>Fang, Li-Jing</creatorcontrib><creatorcontrib>Ji, Xian-Ling</creatorcontrib><title>Integrated Phloem Sap mRNA and Protein Expression Analysis Reveals Phytoplasma-infection Responses in Mulberry</title><title>Molecular & cellular proteomics</title><addtitle>Mol Cell Proteomics</addtitle><description>To gain insight into the response of mulberry to phytoplasma-infection, the expression profiles of mRNAs and proteins in mulberry phloem sap were examined. A total of 955 unigenes and 136 proteins were found to be differentially expressed between the healthy and infected phloem sap. These differentially expressed mRNAs and proteins are involved in signaling, hormone metabolism, stress responses, etc. Interestingly, we found that both the mRNA and protein levels of the major latex protein-like 329 (MuMLPL329) gene were increased in the infected phloem saps. Expression of the MuMLPL329 gene was induced by pathogen inoculation and was responsive to jasmonic acid. Ectopic expression of MuMLPL329 in Arabidopsis enhances transgenic plant resistance to Botrytis cinerea, Pseudomonas syringae pv tomato DC3000 (Pst. DC3000) and phytoplasma. Further analysis revealed that MuMLPL329 can enhance the expression of some defense genes and might be involved in altering flavonoid content resulting in increased resistance of plants to pathogen infection. Finally, the roles of the differentially expressed mRNAs and proteins and the potential molecular mechanisms of their changes were discussed. It was likely that the phytoplasma-responsive mRNAs and proteins in the phloem saps were involved in multiple pathways of mulberry responses to phytoplasma-infection, and their changes may be partially responsible for some symptoms in the phytoplasma infected plants.</description><subject>Amino Acid Sequence</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - microbiology</subject><subject>Down-Regulation - genetics</subject><subject>Flavonoids - analysis</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Ontology</subject><subject>Genes, Plant</subject><subject>Host-Pathogen Interaction</subject><subject>iTRAQ</subject><subject>Molecular biology</subject><subject>Morus - genetics</subject><subject>Morus - metabolism</subject><subject>Morus - microbiology</subject><subject>mulberry</subject><subject>Nicotiana - genetics</subject><subject>Phenotype</subject><subject>Phloem - metabolism</subject><subject>Phloem - microbiology</subject><subject>phloem sap</subject><subject>Phylogeny</subject><subject>phytoplasm</subject><subject>Phytoplasma - physiology</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>proteome</subject><subject>Pseudomonas syringae - physiology</subject><subject>Ribulose-Bisphosphate Carboxylase - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Stress response</subject><subject>transcriptome</subject><subject>Up-Regulation - genetics</subject><issn>1535-9476</issn><issn>1535-9484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFP2zAYhq2JaTDYnRPKkUuKnTixzQGpqmBDYhvqtrPlOF_AKLGDv7Si_x6zsmocONmyn_e19T2EHDM6Y1Tws8GOs-WcMTmjlNaCfiAHrCqrXHHJ93Z7Ue-Tz4gPlBaUieoT2S-U5FLI8oD4az_BXTQTtNntfR9gyH6ZMRuWP-aZ8ekshgmczy6fxgiILvhs7k2_QYfZEtZgeky5zRTG3uBgcuc7sNMLtgQcg0fALMW_r_oGYtwckY9disCX1_WQ_Lm6_L34lt_8_Hq9mN_klnMx5bLltqtaRpW0rWxKoZRRtQAJnHPFRGNN09kKGCs5p23dVKWShW06BoVqjCkPycW2d1w1A7QW_BRNr8foBhM3Ohin3954d6_vwlrXrKhLxVLB6WtBDI8rwEkPDi30vfEQVqgLyoViSlUyoXSL2hgQI3S7ZxjVL5p00qT_atJbTSly8v_3doF_XhJwvgUgDWntIGq0DryF1sU0X90G9377M8OdpV8</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Gai, Ying-Ping</creator><creator>Yuan, Shuo-Shuo</creator><creator>Liu, Zhao-Yang</creator><creator>Zhao, Huai-Ning</creator><creator>Liu, Qi</creator><creator>Qin, Rong-Li</creator><creator>Fang, Li-Jing</creator><creator>Ji, Xian-Ling</creator><general>Elsevier Inc</general><general>The American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180901</creationdate><title>Integrated Phloem Sap mRNA and Protein Expression Analysis Reveals Phytoplasma-infection Responses in Mulberry</title><author>Gai, Ying-Ping ; Yuan, Shuo-Shuo ; Liu, Zhao-Yang ; Zhao, Huai-Ning ; Liu, Qi ; Qin, Rong-Li ; Fang, Li-Jing ; Ji, Xian-Ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-8d4cf5d1098cd8b3799a967e8e444917bcabfc5e113440d6b53982cbf1e29baa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amino Acid Sequence</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - microbiology</topic><topic>Down-Regulation - genetics</topic><topic>Flavonoids - analysis</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Ontology</topic><topic>Genes, Plant</topic><topic>Host-Pathogen Interaction</topic><topic>iTRAQ</topic><topic>Molecular biology</topic><topic>Morus - genetics</topic><topic>Morus - metabolism</topic><topic>Morus - microbiology</topic><topic>mulberry</topic><topic>Nicotiana - genetics</topic><topic>Phenotype</topic><topic>Phloem - metabolism</topic><topic>Phloem - microbiology</topic><topic>phloem sap</topic><topic>Phylogeny</topic><topic>phytoplasm</topic><topic>Phytoplasma - physiology</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>proteome</topic><topic>Pseudomonas syringae - physiology</topic><topic>Ribulose-Bisphosphate Carboxylase - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Stress response</topic><topic>transcriptome</topic><topic>Up-Regulation - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gai, Ying-Ping</creatorcontrib><creatorcontrib>Yuan, Shuo-Shuo</creatorcontrib><creatorcontrib>Liu, Zhao-Yang</creatorcontrib><creatorcontrib>Zhao, Huai-Ning</creatorcontrib><creatorcontrib>Liu, Qi</creatorcontrib><creatorcontrib>Qin, Rong-Li</creatorcontrib><creatorcontrib>Fang, Li-Jing</creatorcontrib><creatorcontrib>Ji, Xian-Ling</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular & cellular proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gai, Ying-Ping</au><au>Yuan, Shuo-Shuo</au><au>Liu, Zhao-Yang</au><au>Zhao, Huai-Ning</au><au>Liu, Qi</au><au>Qin, Rong-Li</au><au>Fang, Li-Jing</au><au>Ji, Xian-Ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrated Phloem Sap mRNA and Protein Expression Analysis Reveals Phytoplasma-infection Responses in Mulberry</atitle><jtitle>Molecular & cellular proteomics</jtitle><addtitle>Mol Cell Proteomics</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>17</volume><issue>9</issue><spage>1702</spage><epage>1719</epage><pages>1702-1719</pages><issn>1535-9476</issn><eissn>1535-9484</eissn><abstract>To gain insight into the response of mulberry to phytoplasma-infection, the expression profiles of mRNAs and proteins in mulberry phloem sap were examined. A total of 955 unigenes and 136 proteins were found to be differentially expressed between the healthy and infected phloem sap. These differentially expressed mRNAs and proteins are involved in signaling, hormone metabolism, stress responses, etc. Interestingly, we found that both the mRNA and protein levels of the major latex protein-like 329 (MuMLPL329) gene were increased in the infected phloem saps. Expression of the MuMLPL329 gene was induced by pathogen inoculation and was responsive to jasmonic acid. Ectopic expression of MuMLPL329 in Arabidopsis enhances transgenic plant resistance to Botrytis cinerea, Pseudomonas syringae pv tomato DC3000 (Pst. DC3000) and phytoplasma. Further analysis revealed that MuMLPL329 can enhance the expression of some defense genes and might be involved in altering flavonoid content resulting in increased resistance of plants to pathogen infection. Finally, the roles of the differentially expressed mRNAs and proteins and the potential molecular mechanisms of their changes were discussed. It was likely that the phytoplasma-responsive mRNAs and proteins in the phloem saps were involved in multiple pathways of mulberry responses to phytoplasma-infection, and their changes may be partially responsible for some symptoms in the phytoplasma infected plants.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29848783</pmid><doi>10.1074/mcp.RA118.000670</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Arabidopsis - genetics Arabidopsis - microbiology Down-Regulation - genetics Flavonoids - analysis Gene Expression Gene Expression Regulation, Plant Gene Ontology Genes, Plant Host-Pathogen Interaction iTRAQ Molecular biology Morus - genetics Morus - metabolism Morus - microbiology mulberry Nicotiana - genetics Phenotype Phloem - metabolism Phloem - microbiology phloem sap Phylogeny phytoplasm Phytoplasma - physiology Plant Diseases - microbiology Plant Leaves - metabolism Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified proteome Pseudomonas syringae - physiology Ribulose-Bisphosphate Carboxylase - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Stress response transcriptome Up-Regulation - genetics |
| title | Integrated Phloem Sap mRNA and Protein Expression Analysis Reveals Phytoplasma-infection Responses in Mulberry |
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