Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line
Choriocarcinoma is a malignant trophoblastic tumor. The development of novel drugs is required to reduce the toxicity of current multi-agent chemotherapy and to successfully treat chemoresistant cases of the disease. The purpose of the present study was to investigate the effect of dihydromyricetin...
Gespeichert in:
| Veröffentlicht in: | Oncology letters 2018-10, Vol.16 (4), p.4229-4234 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4234 |
|---|---|
| container_issue | 4 |
| container_start_page | 4229 |
| container_title | Oncology letters |
| container_volume | 16 |
| creator | Zuo, Yanzhen Xu, Qian Lu, Yanjie Sun, Dayong Wang, Kang Lei, Yuntao Liang, Xiujun Li, Yuhong |
| description | Choriocarcinoma is a malignant trophoblastic tumor. The development of novel drugs is required to reduce the toxicity of current multi-agent chemotherapy and to successfully treat chemoresistant cases of the disease. The purpose of the present study was to investigate the effect of dihydromyricetin (DMY) on the human choriocarcinoma cell line, JAr, to identify a novel drug for the treatment of choriocarcinoma. An MTT assay was performed to determine the effects of DMY at different concentrations and for different exposure durations. Flow cytometry and TUNEL assays were performed to detect apoptosis, and western blotting was utilized to investigate the underlying mechanism. The results revealed that DMY significantly inhibited JAr cell viability in a time- and dose-dependent manner. The flow cytometry and TUNEL assays demonstrated that DMY inhibited proliferation by inducing apoptosis. Further analysis by western blotting indicated that the protein expression level of BCL-2 associated X, associated protein increased, while the protein expression levels of BCL-2 and pro-caspase-3 decreased. These findings suggest that DMY induced apoptosis in human choriocarcinoma JAr cells, through a mitochondrially mediated apoptotic pathway. |
| doi_str_mv | 10.3892/ol.2018.9220 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6126223</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A556870975</galeid><sourcerecordid>A556870975</sourcerecordid><originalsourceid>FETCH-LOGICAL-c510t-8ff973ea9913000fd42ac99b591f9850edb0c244bf1bf503e1df8613aaa95cae3</originalsourceid><addsrcrecordid>eNptkc9rFTEQx4MottTePMuC4Mn3zI_NbnIRStUqFLzoOcxmk25KNvNMdoX335tH67MPTA4ZZj7zZTJfQl4zuhVK8w8Yt5wytdWc02fknPWabxhV_Pkx7tszclnKPa1Hdkyp7iU5E5SzVkp1Tm4-hWk_Zpz3OVi3hNSENK7WlQZ2uFuwhFIzDTTTOkNq7IQ5oIVsQ8IZGutibGJI7hV54SEWd_n4XpCfXz7_uP66uf1-8-366nZjJaPLRnmve-FAaybqQH5sOVitB6mZ10pSNw7U8rYdPBu8pMKx0auOCQDQ0oITF-Tjg-5uHWY3WpeWDNHscpgh7w1CMKeVFCZzh79Nx3jHuagCbx8FMv5aXVnMPa451ZkNZ1TTnnLa_aPuIDoTkscqZudQrLmSslM91b2s1PY_VL2jm4PF5Hyo-ZOGd08aJgdxmQrGdQmYyin4_gG0GUvJzh9_yKg5OG8wmoPz5uB8xd883coR_uuz-APliKg3</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2109070206</pqid></control><display><type>article</type><title>Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line</title><source>Spandidos Publications Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Zuo, Yanzhen ; Xu, Qian ; Lu, Yanjie ; Sun, Dayong ; Wang, Kang ; Lei, Yuntao ; Liang, Xiujun ; Li, Yuhong</creator><creatorcontrib>Zuo, Yanzhen ; Xu, Qian ; Lu, Yanjie ; Sun, Dayong ; Wang, Kang ; Lei, Yuntao ; Liang, Xiujun ; Li, Yuhong</creatorcontrib><description>Choriocarcinoma is a malignant trophoblastic tumor. The development of novel drugs is required to reduce the toxicity of current multi-agent chemotherapy and to successfully treat chemoresistant cases of the disease. The purpose of the present study was to investigate the effect of dihydromyricetin (DMY) on the human choriocarcinoma cell line, JAr, to identify a novel drug for the treatment of choriocarcinoma. An MTT assay was performed to determine the effects of DMY at different concentrations and for different exposure durations. Flow cytometry and TUNEL assays were performed to detect apoptosis, and western blotting was utilized to investigate the underlying mechanism. The results revealed that DMY significantly inhibited JAr cell viability in a time- and dose-dependent manner. The flow cytometry and TUNEL assays demonstrated that DMY inhibited proliferation by inducing apoptosis. Further analysis by western blotting indicated that the protein expression level of BCL-2 associated X, associated protein increased, while the protein expression levels of BCL-2 and pro-caspase-3 decreased. These findings suggest that DMY induced apoptosis in human choriocarcinoma JAr cells, through a mitochondrially mediated apoptotic pathway.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2018.9220</identifier><identifier>PMID: 30214558</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Apoptosis ; Cancer therapies ; Care and treatment ; Chemotherapy ; Choriocarcinoma ; Development and progression ; Drug dosages ; Flavonoids ; Flow cytometry ; Health aspects ; Lung cancer ; Morphology ; Oncology ; Protein expression ; Proteins</subject><ispartof>Oncology letters, 2018-10, Vol.16 (4), p.4229-4234</ispartof><rights>COPYRIGHT 2018 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright: © Zuo et al. 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-8ff973ea9913000fd42ac99b591f9850edb0c244bf1bf503e1df8613aaa95cae3</citedby><cites>FETCH-LOGICAL-c510t-8ff973ea9913000fd42ac99b591f9850edb0c244bf1bf503e1df8613aaa95cae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126223/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126223/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30214558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zuo, Yanzhen</creatorcontrib><creatorcontrib>Xu, Qian</creatorcontrib><creatorcontrib>Lu, Yanjie</creatorcontrib><creatorcontrib>Sun, Dayong</creatorcontrib><creatorcontrib>Wang, Kang</creatorcontrib><creatorcontrib>Lei, Yuntao</creatorcontrib><creatorcontrib>Liang, Xiujun</creatorcontrib><creatorcontrib>Li, Yuhong</creatorcontrib><title>Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>Choriocarcinoma is a malignant trophoblastic tumor. The development of novel drugs is required to reduce the toxicity of current multi-agent chemotherapy and to successfully treat chemoresistant cases of the disease. The purpose of the present study was to investigate the effect of dihydromyricetin (DMY) on the human choriocarcinoma cell line, JAr, to identify a novel drug for the treatment of choriocarcinoma. An MTT assay was performed to determine the effects of DMY at different concentrations and for different exposure durations. Flow cytometry and TUNEL assays were performed to detect apoptosis, and western blotting was utilized to investigate the underlying mechanism. The results revealed that DMY significantly inhibited JAr cell viability in a time- and dose-dependent manner. The flow cytometry and TUNEL assays demonstrated that DMY inhibited proliferation by inducing apoptosis. Further analysis by western blotting indicated that the protein expression level of BCL-2 associated X, associated protein increased, while the protein expression levels of BCL-2 and pro-caspase-3 decreased. These findings suggest that DMY induced apoptosis in human choriocarcinoma JAr cells, through a mitochondrially mediated apoptotic pathway.</description><subject>Apoptosis</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Chemotherapy</subject><subject>Choriocarcinoma</subject><subject>Development and progression</subject><subject>Drug dosages</subject><subject>Flavonoids</subject><subject>Flow cytometry</subject><subject>Health aspects</subject><subject>Lung cancer</subject><subject>Morphology</subject><subject>Oncology</subject><subject>Protein expression</subject><subject>Proteins</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkc9rFTEQx4MottTePMuC4Mn3zI_NbnIRStUqFLzoOcxmk25KNvNMdoX335tH67MPTA4ZZj7zZTJfQl4zuhVK8w8Yt5wytdWc02fknPWabxhV_Pkx7tszclnKPa1Hdkyp7iU5E5SzVkp1Tm4-hWk_Zpz3OVi3hNSENK7WlQZ2uFuwhFIzDTTTOkNq7IQ5oIVsQ8IZGutibGJI7hV54SEWd_n4XpCfXz7_uP66uf1-8-366nZjJaPLRnmve-FAaybqQH5sOVitB6mZ10pSNw7U8rYdPBu8pMKx0auOCQDQ0oITF-Tjg-5uHWY3WpeWDNHscpgh7w1CMKeVFCZzh79Nx3jHuagCbx8FMv5aXVnMPa451ZkNZ1TTnnLa_aPuIDoTkscqZudQrLmSslM91b2s1PY_VL2jm4PF5Hyo-ZOGd08aJgdxmQrGdQmYyin4_gG0GUvJzh9_yKg5OG8wmoPz5uB8xd883coR_uuz-APliKg3</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Zuo, Yanzhen</creator><creator>Xu, Qian</creator><creator>Lu, Yanjie</creator><creator>Sun, Dayong</creator><creator>Wang, Kang</creator><creator>Lei, Yuntao</creator><creator>Liang, Xiujun</creator><creator>Li, Yuhong</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20181001</creationdate><title>Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line</title><author>Zuo, Yanzhen ; Xu, Qian ; Lu, Yanjie ; Sun, Dayong ; Wang, Kang ; Lei, Yuntao ; Liang, Xiujun ; Li, Yuhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-8ff973ea9913000fd42ac99b591f9850edb0c244bf1bf503e1df8613aaa95cae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apoptosis</topic><topic>Cancer therapies</topic><topic>Care and treatment</topic><topic>Chemotherapy</topic><topic>Choriocarcinoma</topic><topic>Development and progression</topic><topic>Drug dosages</topic><topic>Flavonoids</topic><topic>Flow cytometry</topic><topic>Health aspects</topic><topic>Lung cancer</topic><topic>Morphology</topic><topic>Oncology</topic><topic>Protein expression</topic><topic>Proteins</topic><toplevel>online_resources</toplevel><creatorcontrib>Zuo, Yanzhen</creatorcontrib><creatorcontrib>Xu, Qian</creatorcontrib><creatorcontrib>Lu, Yanjie</creatorcontrib><creatorcontrib>Sun, Dayong</creatorcontrib><creatorcontrib>Wang, Kang</creatorcontrib><creatorcontrib>Lei, Yuntao</creatorcontrib><creatorcontrib>Liang, Xiujun</creatorcontrib><creatorcontrib>Li, Yuhong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zuo, Yanzhen</au><au>Xu, Qian</au><au>Lu, Yanjie</au><au>Sun, Dayong</au><au>Wang, Kang</au><au>Lei, Yuntao</au><au>Liang, Xiujun</au><au>Li, Yuhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line</atitle><jtitle>Oncology letters</jtitle><addtitle>Oncol Lett</addtitle><date>2018-10-01</date><risdate>2018</risdate><volume>16</volume><issue>4</issue><spage>4229</spage><epage>4234</epage><pages>4229-4234</pages><issn>1792-1074</issn><eissn>1792-1082</eissn><abstract>Choriocarcinoma is a malignant trophoblastic tumor. The development of novel drugs is required to reduce the toxicity of current multi-agent chemotherapy and to successfully treat chemoresistant cases of the disease. The purpose of the present study was to investigate the effect of dihydromyricetin (DMY) on the human choriocarcinoma cell line, JAr, to identify a novel drug for the treatment of choriocarcinoma. An MTT assay was performed to determine the effects of DMY at different concentrations and for different exposure durations. Flow cytometry and TUNEL assays were performed to detect apoptosis, and western blotting was utilized to investigate the underlying mechanism. The results revealed that DMY significantly inhibited JAr cell viability in a time- and dose-dependent manner. The flow cytometry and TUNEL assays demonstrated that DMY inhibited proliferation by inducing apoptosis. Further analysis by western blotting indicated that the protein expression level of BCL-2 associated X, associated protein increased, while the protein expression levels of BCL-2 and pro-caspase-3 decreased. These findings suggest that DMY induced apoptosis in human choriocarcinoma JAr cells, through a mitochondrially mediated apoptotic pathway.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>30214558</pmid><doi>10.3892/ol.2018.9220</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-1074 |
| ispartof | Oncology letters, 2018-10, Vol.16 (4), p.4229-4234 |
| issn | 1792-1074 1792-1082 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6126223 |
| source | Spandidos Publications Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Apoptosis Cancer therapies Care and treatment Chemotherapy Choriocarcinoma Development and progression Drug dosages Flavonoids Flow cytometry Health aspects Lung cancer Morphology Oncology Protein expression Proteins |
| title | Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T10%3A05%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dihydromyricetin%20induces%20apoptosis%20in%20a%20human%20choriocarcinoma%20cell%20line&rft.jtitle=Oncology%20letters&rft.au=Zuo,%20Yanzhen&rft.date=2018-10-01&rft.volume=16&rft.issue=4&rft.spage=4229&rft.epage=4234&rft.pages=4229-4234&rft.issn=1792-1074&rft.eissn=1792-1082&rft_id=info:doi/10.3892/ol.2018.9220&rft_dat=%3Cgale_pubme%3EA556870975%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2109070206&rft_id=info:pmid/30214558&rft_galeid=A556870975&rfr_iscdi=true |