Effect of ranibizumab on levels of IL-6 and VEGF in peripheral blood and aqueous humor of glaucoma rat model and association of IL-6 and VEGF with optic nerve damage
This study aims to investigate the effect of intravitreal injection of ranibizumab on glaucoma rat model and its effect on optic nerve injury. A total of 125 SD rats were used to establish glaucoma models. Of them, 80 well-established model were selected, 40 of which received intravitreal injections...
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| creator | Song, Yanling Song, Qiujie Li, Ling Xu, Jinfeng Liu, Xingqiang |
| description | This study aims to investigate the effect of intravitreal injection of ranibizumab on glaucoma rat model and its effect on optic nerve injury. A total of 125 SD rats were used to establish glaucoma models. Of them, 80 well-established model were selected, 40 of which received intravitreal injections of ranibizumab and were assigned to the observation group. The remaining 40 received no drug and were assigned to the control group. After rats were sacrificed at the time-points, the retina was harvested. Numbers of retinal ganglion cells (RGCs) were counted under a fluorescence microscope. In the meantime, levels of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) in peripheral blood and aqueous humor were determined. The levels of IL-6 and VEGF in peripheral blood and aqueous humor decreased gradually over the time of treatment in the observation group (P |
| doi_str_mv | 10.3892/etm.2018.6441 |
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A total of 125 SD rats were used to establish glaucoma models. Of them, 80 well-established model were selected, 40 of which received intravitreal injections of ranibizumab and were assigned to the observation group. The remaining 40 received no drug and were assigned to the control group. After rats were sacrificed at the time-points, the retina was harvested. Numbers of retinal ganglion cells (RGCs) were counted under a fluorescence microscope. In the meantime, levels of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) in peripheral blood and aqueous humor were determined. The levels of IL-6 and VEGF in peripheral blood and aqueous humor decreased gradually over the time of treatment in the observation group (P<0.05). In the control group the levels of IL-6 in peripheral blood and aqueous humor increased gradually over time (P<0.05). At the same time-point, the IL-6 level was higher in the control group than that in the observation group (P<0.05). The VEGF level in the observation group was lower in day 21 than that in day 7 (P<0.05). The VEGF level was higher in the control group than that in the observation group (P<0.05). The number of RGCs in the observation group increased gradually over the time of treatment, and there were significant differences in the number of RGCs between day 7 and 14, as well as day 14 and 21 (P<0.05). The number of RGCs in the control group decreased gradually over time (P<0.05). The levels of IL-6 and VEGF were all negatively correlated with the number of RGCs, and the correlation coefficient r and P-value were -0.743 and 0.012, and -0.675 and 0.022, respectively. Ranibizumab attenuated optic nerve injury by reducing levels of IL-6 and VEGF in peripheral blood and aqueous humor of glaucoma rat model.]]></description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2018.6441</identifier><identifier>PMID: 30210601</identifier><language>eng</language><publisher>Greece: Spandidos Publications UK Ltd</publisher><subject>Apoptosis ; Cytokines ; Food ; Glaucoma ; Immune system ; Immunotherapy ; Lasers ; Males ; Monoclonal antibodies ; Optic nerve ; Pathogenesis ; Rodents ; Studies ; Vascular endothelial growth factor</subject><ispartof>Experimental and therapeutic medicine, 2018-09, Vol.16 (3), p.2506-2510</ispartof><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright: © Song et al. 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-d299e26ff01b16a704fc1f0152b9c3c6fd08bb5cb996998f7cac9a8faf6dc6333</citedby><cites>FETCH-LOGICAL-c415t-d299e26ff01b16a704fc1f0152b9c3c6fd08bb5cb996998f7cac9a8faf6dc6333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122519/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122519/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30210601$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Yanling</creatorcontrib><creatorcontrib>Song, Qiujie</creatorcontrib><creatorcontrib>Li, Ling</creatorcontrib><creatorcontrib>Xu, Jinfeng</creatorcontrib><creatorcontrib>Liu, Xingqiang</creatorcontrib><title>Effect of ranibizumab on levels of IL-6 and VEGF in peripheral blood and aqueous humor of glaucoma rat model and association of IL-6 and VEGF with optic nerve damage</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description><![CDATA[This study aims to investigate the effect of intravitreal injection of ranibizumab on glaucoma rat model and its effect on optic nerve injury. A total of 125 SD rats were used to establish glaucoma models. Of them, 80 well-established model were selected, 40 of which received intravitreal injections of ranibizumab and were assigned to the observation group. The remaining 40 received no drug and were assigned to the control group. After rats were sacrificed at the time-points, the retina was harvested. Numbers of retinal ganglion cells (RGCs) were counted under a fluorescence microscope. In the meantime, levels of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) in peripheral blood and aqueous humor were determined. The levels of IL-6 and VEGF in peripheral blood and aqueous humor decreased gradually over the time of treatment in the observation group (P<0.05). In the control group the levels of IL-6 in peripheral blood and aqueous humor increased gradually over time (P<0.05). At the same time-point, the IL-6 level was higher in the control group than that in the observation group (P<0.05). The VEGF level in the observation group was lower in day 21 than that in day 7 (P<0.05). The VEGF level was higher in the control group than that in the observation group (P<0.05). The number of RGCs in the observation group increased gradually over the time of treatment, and there were significant differences in the number of RGCs between day 7 and 14, as well as day 14 and 21 (P<0.05). The number of RGCs in the control group decreased gradually over time (P<0.05). The levels of IL-6 and VEGF were all negatively correlated with the number of RGCs, and the correlation coefficient r and P-value were -0.743 and 0.012, and -0.675 and 0.022, respectively. Ranibizumab attenuated optic nerve injury by reducing levels of IL-6 and VEGF in peripheral blood and aqueous humor of glaucoma rat model.]]></description><subject>Apoptosis</subject><subject>Cytokines</subject><subject>Food</subject><subject>Glaucoma</subject><subject>Immune system</subject><subject>Immunotherapy</subject><subject>Lasers</subject><subject>Males</subject><subject>Monoclonal antibodies</subject><subject>Optic nerve</subject><subject>Pathogenesis</subject><subject>Rodents</subject><subject>Studies</subject><subject>Vascular endothelial growth factor</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNplkkuPFCEUhStG40zGWbo1JG7cVMuji4KNiZn0PJJO3KhbAhR0MymgBKon-n_8n1LT7cQHGyD348C5nKZ5jeCKMI7fm-JXGCK2ous1etaco57jFkHUPT-tIWforLnM-R7W0VHEWPeyOSMQI0ghOm9-bqw1uoBoQZLBKfdj9lKBGMBoDmbMS-Fu21IgwwC-bm6ugQtgMslNe5PkCNQY4_BYlN9mE-cM9rOPaTm2G-Wso5dVuAAfBzMeuZyjdrK4esd_4g-u7EGcitMgmHQwYJBe7syr5oWVYzaXp_mi-XK9-Xx1224_3dxdfdy2eo260g6Yc4OptRApRGUP11ajuumw4ppoagfIlOq04pxyzmyvpeaSWWnpoCkh5KL5cNSdZuXNoE0o1aSYkvMyfRdROvF3Jbi92MWDoAjjDvEq8O4kkGLtRy7Cu6zNOMqwNEfUthPaQ8JxRd_-g97HOYVqT2DIOUF9xxaqPVI6xZyTsU-PQVAsGRA1A2LJgFgyUPk3fzp4on__OPkFfaqt-Q</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Song, Yanling</creator><creator>Song, Qiujie</creator><creator>Li, Ling</creator><creator>Xu, Jinfeng</creator><creator>Liu, Xingqiang</creator><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180901</creationdate><title>Effect of ranibizumab on levels of IL-6 and VEGF in peripheral blood and aqueous humor of glaucoma rat model and association of IL-6 and VEGF with optic nerve damage</title><author>Song, Yanling ; Song, Qiujie ; Li, Ling ; Xu, Jinfeng ; Liu, Xingqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-d299e26ff01b16a704fc1f0152b9c3c6fd08bb5cb996998f7cac9a8faf6dc6333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apoptosis</topic><topic>Cytokines</topic><topic>Food</topic><topic>Glaucoma</topic><topic>Immune system</topic><topic>Immunotherapy</topic><topic>Lasers</topic><topic>Males</topic><topic>Monoclonal antibodies</topic><topic>Optic nerve</topic><topic>Pathogenesis</topic><topic>Rodents</topic><topic>Studies</topic><topic>Vascular endothelial growth factor</topic><toplevel>online_resources</toplevel><creatorcontrib>Song, Yanling</creatorcontrib><creatorcontrib>Song, Qiujie</creatorcontrib><creatorcontrib>Li, Ling</creatorcontrib><creatorcontrib>Xu, Jinfeng</creatorcontrib><creatorcontrib>Liu, Xingqiang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Yanling</au><au>Song, Qiujie</au><au>Li, Ling</au><au>Xu, Jinfeng</au><au>Liu, Xingqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of ranibizumab on levels of IL-6 and VEGF in peripheral blood and aqueous humor of glaucoma rat model and association of IL-6 and VEGF with optic nerve damage</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>16</volume><issue>3</issue><spage>2506</spage><epage>2510</epage><pages>2506-2510</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract><![CDATA[This study aims to investigate the effect of intravitreal injection of ranibizumab on glaucoma rat model and its effect on optic nerve injury. A total of 125 SD rats were used to establish glaucoma models. Of them, 80 well-established model were selected, 40 of which received intravitreal injections of ranibizumab and were assigned to the observation group. The remaining 40 received no drug and were assigned to the control group. After rats were sacrificed at the time-points, the retina was harvested. Numbers of retinal ganglion cells (RGCs) were counted under a fluorescence microscope. In the meantime, levels of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) in peripheral blood and aqueous humor were determined. The levels of IL-6 and VEGF in peripheral blood and aqueous humor decreased gradually over the time of treatment in the observation group (P<0.05). In the control group the levels of IL-6 in peripheral blood and aqueous humor increased gradually over time (P<0.05). At the same time-point, the IL-6 level was higher in the control group than that in the observation group (P<0.05). The VEGF level in the observation group was lower in day 21 than that in day 7 (P<0.05). The VEGF level was higher in the control group than that in the observation group (P<0.05). The number of RGCs in the observation group increased gradually over the time of treatment, and there were significant differences in the number of RGCs between day 7 and 14, as well as day 14 and 21 (P<0.05). The number of RGCs in the control group decreased gradually over time (P<0.05). The levels of IL-6 and VEGF were all negatively correlated with the number of RGCs, and the correlation coefficient r and P-value were -0.743 and 0.012, and -0.675 and 0.022, respectively. Ranibizumab attenuated optic nerve injury by reducing levels of IL-6 and VEGF in peripheral blood and aqueous humor of glaucoma rat model.]]></abstract><cop>Greece</cop><pub>Spandidos Publications UK Ltd</pub><pmid>30210601</pmid><doi>10.3892/etm.2018.6441</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central |
| subjects | Apoptosis Cytokines Food Glaucoma Immune system Immunotherapy Lasers Males Monoclonal antibodies Optic nerve Pathogenesis Rodents Studies Vascular endothelial growth factor |
| title | Effect of ranibizumab on levels of IL-6 and VEGF in peripheral blood and aqueous humor of glaucoma rat model and association of IL-6 and VEGF with optic nerve damage |
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