The expression of MMP‐1 and MMP‐9 is up‐regulated by smooth muscle cells after their cross‐talk with macrophages in high glucose conditions

Patients with diabetes mellitus have an increased risk of myocardial infarction and coronary artery disease‐related death, exhibiting highly vulnerable plaques. Many studies have highlighted the major role of macrophages (MAC) and smooth muscle cells (SMC) and the essential part of metalloproteases...

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Veröffentlicht in:Journal of cellular and molecular medicine 2018-09, Vol.22 (9), p.4366-4376
Hauptverfasser: Macarie, Razvan Daniel, Vadana, Mihaela, Ciortan, Letitia, Tucureanu, Monica M., Ciobanu, Andrea, Vinereanu, Dragos, Manduteanu, Ileana, Simionescu, Maya, Butoi, Elena
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container_issue 9
container_start_page 4366
container_title Journal of cellular and molecular medicine
container_volume 22
creator Macarie, Razvan Daniel
Vadana, Mihaela
Ciortan, Letitia
Tucureanu, Monica M.
Ciobanu, Andrea
Vinereanu, Dragos
Manduteanu, Ileana
Simionescu, Maya
Butoi, Elena
description Patients with diabetes mellitus have an increased risk of myocardial infarction and coronary artery disease‐related death, exhibiting highly vulnerable plaques. Many studies have highlighted the major role of macrophages (MAC) and smooth muscle cells (SMC) and the essential part of metalloproteases (MMPs) in atherosclerotic plaque vulnerability. We hypothesize that in diabetes, the interplay between MAC and SMC in high glucose conditions may modify the expression of MMPs involved in plaque vulnerability. The SMC‐MAC cross‐talk was achieved using trans‐well chambers, where human SMC were grown at the bottom and human MAC in the upper chamber in normal (NG) or high (HG) glucose concentration. After cross‐talk, the conditioned media and cells were isolated and investigated for the expression of MMPs, MCP‐1 and signalling molecules. We found that upon cross‐talk with MAC in HG, SMC exhibit: (i) augmented expression of MMP‐1 and MMP‐9; (ii) significant increase in the enzymatic activity of MMP‐9; (iii) higher levels of soluble MCP‐1 chemokine which is functionally active and involved in MMPs up‐regulation; (iv) activated PKCα signalling pathway which, together with NF‐kB are responsible for MMP‐1 and MMP‐9 up‐regulation, and (v) impaired function of collagen assembly. Taken together, our data indicate that MCP‐1 released by cell cross‐talk in diabetic conditions binds to CCR2 and triggers MMP‐1 and MMP‐9 over‐expression and activity, features that could explain the high vulnerability of atherosclerotic plaque found at diabetic patients.
doi_str_mv 10.1111/jcmm.13728
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Many studies have highlighted the major role of macrophages (MAC) and smooth muscle cells (SMC) and the essential part of metalloproteases (MMPs) in atherosclerotic plaque vulnerability. We hypothesize that in diabetes, the interplay between MAC and SMC in high glucose conditions may modify the expression of MMPs involved in plaque vulnerability. The SMC‐MAC cross‐talk was achieved using trans‐well chambers, where human SMC were grown at the bottom and human MAC in the upper chamber in normal (NG) or high (HG) glucose concentration. After cross‐talk, the conditioned media and cells were isolated and investigated for the expression of MMPs, MCP‐1 and signalling molecules. We found that upon cross‐talk with MAC in HG, SMC exhibit: (i) augmented expression of MMP‐1 and MMP‐9; (ii) significant increase in the enzymatic activity of MMP‐9; (iii) higher levels of soluble MCP‐1 chemokine which is functionally active and involved in MMPs up‐regulation; (iv) activated PKCα signalling pathway which, together with NF‐kB are responsible for MMP‐1 and MMP‐9 up‐regulation, and (v) impaired function of collagen assembly. 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We found that upon cross‐talk with MAC in HG, SMC exhibit: (i) augmented expression of MMP‐1 and MMP‐9; (ii) significant increase in the enzymatic activity of MMP‐9; (iii) higher levels of soluble MCP‐1 chemokine which is functionally active and involved in MMPs up‐regulation; (iv) activated PKCα signalling pathway which, together with NF‐kB are responsible for MMP‐1 and MMP‐9 up‐regulation, and (v) impaired function of collagen assembly. Taken together, our data indicate that MCP‐1 released by cell cross‐talk in diabetic conditions binds to CCR2 and triggers MMP‐1 and MMP‐9 over‐expression and activity, features that could explain the high vulnerability of atherosclerotic plaque found at diabetic patients.</abstract><cop>England</cop><pub>John Wiley &amp; Sons, Inc</pub><pmid>29992758</pmid><doi>10.1111/jcmm.13728</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-5748-5641</orcidid><oa>free_for_read</oa></addata></record>
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subjects Aorta - cytology
Aorta - metabolism
Arteriosclerosis
Atherosclerosis
Cardiovascular disease
CCR2 protein
cell cross‐talk
Chemokine CCL2 - genetics
Chemokine CCL2 - metabolism
Coculture Techniques
Collagen
Collagen - genetics
Collagen - metabolism
Conditioning
Coronary artery
Coronary artery disease
Culture Media, Conditioned - chemistry
Diabetes
Diabetes mellitus
Diffusion Chambers, Culture
Enzymatic activity
Fetus
Gene Expression Regulation
Glucose
Glucose - metabolism
Glucose - pharmacology
Health risks
Heart diseases
high glucose
Humans
Macrophages
Matrix Metalloproteinase 1 - genetics
Matrix Metalloproteinase 1 - metabolism
Matrix Metalloproteinase 9 - genetics
Matrix Metalloproteinase 9 - metabolism
matrix metalloproteinases
Monocyte chemoattractant protein 1
Monocytes - cytology
Monocytes - drug effects
Monocytes - metabolism
Muscles
Myocardial infarction
Myocytes, Smooth Muscle - cytology
Myocytes, Smooth Muscle - drug effects
Myocytes, Smooth Muscle - metabolism
NF-kappa B - genetics
NF-kappa B - metabolism
Original
Plaques
Primary Cell Culture
Protein kinase C
Protein Kinase C-alpha - genetics
Protein Kinase C-alpha - metabolism
Signal Transduction
Smooth muscle
THP-1 Cells
title The expression of MMP‐1 and MMP‐9 is up‐regulated by smooth muscle cells after their cross‐talk with macrophages in high glucose conditions
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T01%3A57%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20expression%20of%20MMP%E2%80%901%20and%20MMP%E2%80%909%20is%20up%E2%80%90regulated%20by%20smooth%20muscle%20cells%20after%20their%20cross%E2%80%90talk%20with%20macrophages%20in%20high%20glucose%20conditions&rft.jtitle=Journal%20of%20cellular%20and%20molecular%20medicine&rft.au=Macarie,%20Razvan%20Daniel&rft.date=2018-09&rft.volume=22&rft.issue=9&rft.spage=4366&rft.epage=4376&rft.pages=4366-4376&rft.issn=1582-1838&rft.eissn=1582-4934&rft_id=info:doi/10.1111/jcmm.13728&rft_dat=%3Cproquest_pubme%3E2068344852%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2094359112&rft_id=info:pmid/29992758&rfr_iscdi=true