Genetic relatedness of Staphylococcus aureus isolates obtained from cystic fibrosis patients at a tertiary academic hospital in Pretoria, South Africa
Cystic fibrosis (CF) is an inherited recessive disease that affects mucocillary clearance in the lung, allowing it to be colonised with bacteria such as Staphylococcus aureus . To survive in the CF lung S. aureus adapts both phenotypically and genotypically, through various mechanisms. In this study...
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| Veröffentlicht in: | Scientific reports 2018-08, Vol.8 (1), p.12222-6, Article 12222 |
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| creator | Goolam Mahomed, T. Kock, M. M. Masekela, R. Hoosien, E. Ehlers, M. M. |
| description | Cystic fibrosis (CF) is an inherited recessive disease that affects mucocillary clearance in the lung, allowing it to be colonised with bacteria such as
Staphylococcus aureus
. To survive in the CF lung
S. aureus
adapts both phenotypically and genotypically, through various mechanisms. In this study, multiple specimens were collected from the participants and were processed routinely and were additionally cultured in chromogenic media. Multiplex PCR assays were employed to detect methicillin resistance and selected virulence and quaternary ammonium compound (
qac
) genes. Genetic relatedness of the
S. aureus
was determined using
agr
, SCC
mec
and
spa
typing as well as pulsed field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Thirty-three
S. aureus
isolates were isolated, of which 51% (17/33) were methicillin resistant
S. aureus
(MRSA). The virulence and
qac
genes were more prevalent in MRSA than the methicillin sensitive
S. aureus
(MSSA) isolates. The PFGE analysis showed nine distinct pulsotypes while MLST showed eight sequence types. All the STs detected in this study, except for ST508 have been previously isolated from CF patients according to the literature. This study showed a genetically diverse
S. aureus
population with a high prevalence of virulence genes among the MRSA isolates from the CF clinic. |
| doi_str_mv | 10.1038/s41598-018-30725-x |
| format | Article |
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Staphylococcus aureus
. To survive in the CF lung
S. aureus
adapts both phenotypically and genotypically, through various mechanisms. In this study, multiple specimens were collected from the participants and were processed routinely and were additionally cultured in chromogenic media. Multiplex PCR assays were employed to detect methicillin resistance and selected virulence and quaternary ammonium compound (
qac
) genes. Genetic relatedness of the
S. aureus
was determined using
agr
, SCC
mec
and
spa
typing as well as pulsed field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Thirty-three
S. aureus
isolates were isolated, of which 51% (17/33) were methicillin resistant
S. aureus
(MRSA). The virulence and
qac
genes were more prevalent in MRSA than the methicillin sensitive
S. aureus
(MSSA) isolates. The PFGE analysis showed nine distinct pulsotypes while MLST showed eight sequence types. All the STs detected in this study, except for ST508 have been previously isolated from CF patients according to the literature. This study showed a genetically diverse
S. aureus
population with a high prevalence of virulence genes among the MRSA isolates from the CF clinic.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-30725-x</identifier><identifier>PMID: 30111773</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>45/22 ; 45/23 ; 45/29 ; 45/41 ; 45/77 ; 631/326/107 ; 692/420/254 ; Ammonium ; Antibiotic resistance ; Cystic fibrosis ; Drug resistance ; Gel electrophoresis ; Gram-positive bacteria ; Humanities and Social Sciences ; Methicillin ; multidisciplinary ; Pathogens ; Science ; Science (multidisciplinary) ; Staphylococcus aureus ; Staphylococcus infections ; Virulence</subject><ispartof>Scientific reports, 2018-08, Vol.8 (1), p.12222-6, Article 12222</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-a2e512a34f91ca01bc0ae876113d8f09b8e1e42e68c540f9ea5d27dc8890973a3</citedby><cites>FETCH-LOGICAL-c474t-a2e512a34f91ca01bc0ae876113d8f09b8e1e42e68c540f9ea5d27dc8890973a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093922/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093922/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51555,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30111773$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goolam Mahomed, T.</creatorcontrib><creatorcontrib>Kock, M. M.</creatorcontrib><creatorcontrib>Masekela, R.</creatorcontrib><creatorcontrib>Hoosien, E.</creatorcontrib><creatorcontrib>Ehlers, M. M.</creatorcontrib><title>Genetic relatedness of Staphylococcus aureus isolates obtained from cystic fibrosis patients at a tertiary academic hospital in Pretoria, South Africa</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Cystic fibrosis (CF) is an inherited recessive disease that affects mucocillary clearance in the lung, allowing it to be colonised with bacteria such as
Staphylococcus aureus
. To survive in the CF lung
S. aureus
adapts both phenotypically and genotypically, through various mechanisms. In this study, multiple specimens were collected from the participants and were processed routinely and were additionally cultured in chromogenic media. Multiplex PCR assays were employed to detect methicillin resistance and selected virulence and quaternary ammonium compound (
qac
) genes. Genetic relatedness of the
S. aureus
was determined using
agr
, SCC
mec
and
spa
typing as well as pulsed field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Thirty-three
S. aureus
isolates were isolated, of which 51% (17/33) were methicillin resistant
S. aureus
(MRSA). The virulence and
qac
genes were more prevalent in MRSA than the methicillin sensitive
S. aureus
(MSSA) isolates. The PFGE analysis showed nine distinct pulsotypes while MLST showed eight sequence types. All the STs detected in this study, except for ST508 have been previously isolated from CF patients according to the literature. This study showed a genetically diverse
S. aureus
population with a high prevalence of virulence genes among the MRSA isolates from the CF clinic.</description><subject>45/22</subject><subject>45/23</subject><subject>45/29</subject><subject>45/41</subject><subject>45/77</subject><subject>631/326/107</subject><subject>692/420/254</subject><subject>Ammonium</subject><subject>Antibiotic resistance</subject><subject>Cystic fibrosis</subject><subject>Drug resistance</subject><subject>Gel electrophoresis</subject><subject>Gram-positive bacteria</subject><subject>Humanities and Social Sciences</subject><subject>Methicillin</subject><subject>multidisciplinary</subject><subject>Pathogens</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Virulence</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1TAQhSNERau2L8ACWWLDgoB_kmt7g1RVUJAqUamwtibOpNdVYgfbqXpfpM9bX24phUW9GUvzzfEZn6p6zegHRoX6mBrWalVTpmpBJW_r2xfVAadNW3PB-csn9_3qOKVrWk7LdcP0q2pfUMaYlOKgujtDj9lZEnGEjL3HlEgYyGWGeb0Zgw3WLonAErEUl8KWKkSXwXnsyRDDROwmbSUG18WQXCIzZIc-l7FMgGSM2UHcELDQ41TAdUizyzAS58lFxByig_fkMix5TU6G6CwcVXsDjAmPH-ph9fPL5x-nX-vz72ffTk_Oa9vIJtfAsWUcRDNoZoGyzlJAJVeMiV4NVHcKGTYcV8q2DR00Qttz2VulNNVSgDisPu1056WbsLfFdYTRzNFNxbEJ4My_He_W5ircmBXVQnNeBN49CMTwa8GUzeSSxXEEj2FJhlOluWpbSQv69j_0OizRl_W2lJJSSy0KxXeULX-ZIg6PZhg12-TNLnlTkje_kze3ZejN0zUeR_7kXACxA1Jp-SuMf99-RvYeYaO9zA</recordid><startdate>20180815</startdate><enddate>20180815</enddate><creator>Goolam Mahomed, T.</creator><creator>Kock, M. 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M.</au><au>Masekela, R.</au><au>Hoosien, E.</au><au>Ehlers, M. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic relatedness of Staphylococcus aureus isolates obtained from cystic fibrosis patients at a tertiary academic hospital in Pretoria, South Africa</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-08-15</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>12222</spage><epage>6</epage><pages>12222-6</pages><artnum>12222</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Cystic fibrosis (CF) is an inherited recessive disease that affects mucocillary clearance in the lung, allowing it to be colonised with bacteria such as
Staphylococcus aureus
. To survive in the CF lung
S. aureus
adapts both phenotypically and genotypically, through various mechanisms. In this study, multiple specimens were collected from the participants and were processed routinely and were additionally cultured in chromogenic media. Multiplex PCR assays were employed to detect methicillin resistance and selected virulence and quaternary ammonium compound (
qac
) genes. Genetic relatedness of the
S. aureus
was determined using
agr
, SCC
mec
and
spa
typing as well as pulsed field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Thirty-three
S. aureus
isolates were isolated, of which 51% (17/33) were methicillin resistant
S. aureus
(MRSA). The virulence and
qac
genes were more prevalent in MRSA than the methicillin sensitive
S. aureus
(MSSA) isolates. The PFGE analysis showed nine distinct pulsotypes while MLST showed eight sequence types. All the STs detected in this study, except for ST508 have been previously isolated from CF patients according to the literature. This study showed a genetically diverse
S. aureus
population with a high prevalence of virulence genes among the MRSA isolates from the CF clinic.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30111773</pmid><doi>10.1038/s41598-018-30725-x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 45/22 45/23 45/29 45/41 45/77 631/326/107 692/420/254 Ammonium Antibiotic resistance Cystic fibrosis Drug resistance Gel electrophoresis Gram-positive bacteria Humanities and Social Sciences Methicillin multidisciplinary Pathogens Science Science (multidisciplinary) Staphylococcus aureus Staphylococcus infections Virulence |
| title | Genetic relatedness of Staphylococcus aureus isolates obtained from cystic fibrosis patients at a tertiary academic hospital in Pretoria, South Africa |
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