A Recombinant Newcastle Disease Virus (NDV) Expressing S Protein of Infectious Bronchitis Virus (IBV) Protects Chickens against IBV and NDV
Infectious bronchitis virus (IBV) causes a highly contagious respiratory, reproductive and urogenital tract disease in chickens worldwide, resulting in substantial economic losses for the poultry industry. Currently, live-attenuated IBV vaccines are used to control the disease. However, safety, atte...
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| Veröffentlicht in: | Scientific reports 2018-08, Vol.8 (1), p.11951-11951, Article 11951 |
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| description | Infectious bronchitis virus (IBV) causes a highly contagious respiratory, reproductive and urogenital tract disease in chickens worldwide, resulting in substantial economic losses for the poultry industry. Currently, live-attenuated IBV vaccines are used to control the disease. However, safety, attenuation and immunization outcomes of current vaccines are not guaranteed. Several studies indicate that attenuated IBV vaccine strains contribute to the emergence of variant viruses in the field due to mutations and recombination. Therefore, there is a need to develop a stable and safe IBV vaccine that will not create variant viruses. In this study, we generated recombinant Newcastle disease viruses (rNDVs) expressing the S1, S2 and S proteins of IBV using reverse genetics technology. Our results showed that the rNDV expressing the S protein of IBV provided better protection than the rNDV expressing S1 or S2 protein of IBV, indicating that the S protein is the best protective antigen of IBV. Immunization of 4-week-old SPF chickens with the rNDV expressing S protein elicited IBV-specific neutralizing antibodies and provided complete protection against virulent IBV and virulent NDV challenges. These results suggest that the rNDV expressing the S protein of IBV is a safe and effective bivalent vaccine candidate for both IBV and NDV. |
| doi_str_mv | 10.1038/s41598-018-30356-2 |
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Currently, live-attenuated IBV vaccines are used to control the disease. However, safety, attenuation and immunization outcomes of current vaccines are not guaranteed. Several studies indicate that attenuated IBV vaccine strains contribute to the emergence of variant viruses in the field due to mutations and recombination. Therefore, there is a need to develop a stable and safe IBV vaccine that will not create variant viruses. In this study, we generated recombinant Newcastle disease viruses (rNDVs) expressing the S1, S2 and S proteins of IBV using reverse genetics technology. Our results showed that the rNDV expressing the S protein of IBV provided better protection than the rNDV expressing S1 or S2 protein of IBV, indicating that the S protein is the best protective antigen of IBV. Immunization of 4-week-old SPF chickens with the rNDV expressing S protein elicited IBV-specific neutralizing antibodies and provided complete protection against virulent IBV and virulent NDV challenges. 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These results suggest that the rNDV expressing the S protein of IBV is a safe and effective bivalent vaccine candidate for both IBV and NDV.</description><subject>13/106</subject><subject>14/63</subject><subject>38/109</subject><subject>38/22</subject><subject>38/23</subject><subject>38/39</subject><subject>38/77</subject><subject>42/44</subject><subject>631/1647/1511</subject><subject>631/1647/664/1881</subject><subject>631/326/590/1867</subject><subject>Animal vaccines</subject><subject>Animals</subject><subject>Antibodies, Neutralizing - metabolism</subject><subject>Antibodies, Viral - metabolism</subject><subject>Bronchitis</subject><subject>Chickens</subject><subject>Chickens - physiology</subject><subject>Coronavirus Infections - immunology</subject><subject>Disease Resistance</subject><subject>Genetics</subject><subject>Humanities and Social Sciences</subject><subject>Immunization</subject><subject>Infectious bronchitis virus - physiology</subject><subject>multidisciplinary</subject><subject>Newcastle disease</subject><subject>Newcastle Disease - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shirvani, Edris</au><au>Paldurai, Anandan</au><au>Manoharan, Vinoth K.</au><au>Varghese, Berin P.</au><au>Samal, Siba K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Recombinant Newcastle Disease Virus (NDV) Expressing S Protein of Infectious Bronchitis Virus (IBV) Protects Chickens against IBV and NDV</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-08-10</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>11951</spage><epage>11951</epage><pages>11951-11951</pages><artnum>11951</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Infectious bronchitis virus (IBV) causes a highly contagious respiratory, reproductive and urogenital tract disease in chickens worldwide, resulting in substantial economic losses for the poultry industry. Currently, live-attenuated IBV vaccines are used to control the disease. However, safety, attenuation and immunization outcomes of current vaccines are not guaranteed. Several studies indicate that attenuated IBV vaccine strains contribute to the emergence of variant viruses in the field due to mutations and recombination. Therefore, there is a need to develop a stable and safe IBV vaccine that will not create variant viruses. In this study, we generated recombinant Newcastle disease viruses (rNDVs) expressing the S1, S2 and S proteins of IBV using reverse genetics technology. Our results showed that the rNDV expressing the S protein of IBV provided better protection than the rNDV expressing S1 or S2 protein of IBV, indicating that the S protein is the best protective antigen of IBV. Immunization of 4-week-old SPF chickens with the rNDV expressing S protein elicited IBV-specific neutralizing antibodies and provided complete protection against virulent IBV and virulent NDV challenges. These results suggest that the rNDV expressing the S protein of IBV is a safe and effective bivalent vaccine candidate for both IBV and NDV.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30097608</pmid><doi>10.1038/s41598-018-30356-2</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/106 14/63 38/109 38/22 38/23 38/39 38/77 42/44 631/1647/1511 631/1647/664/1881 631/326/590/1867 Animal vaccines Animals Antibodies, Neutralizing - metabolism Antibodies, Viral - metabolism Bronchitis Chickens Chickens - physiology Coronavirus Infections - immunology Disease Resistance Genetics Humanities and Social Sciences Immunization Infectious bronchitis virus - physiology multidisciplinary Newcastle disease Newcastle Disease - immunology Newcastle disease virus - physiology Protective antigen Proteins Recombination Reverse Genetics Science Science (multidisciplinary) Spike Glycoprotein, Coronavirus - genetics Vaccines Vaccines, Synthetic Viral Vaccines - genetics Viral Vaccines - immunology Viruses |
| title | A Recombinant Newcastle Disease Virus (NDV) Expressing S Protein of Infectious Bronchitis Virus (IBV) Protects Chickens against IBV and NDV |
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