iTRAQ-Based Proteomics Analysis Reveals the Effect of Rhubarb in Rats with Ischemic Stroke
Background. Rhubarb, a traditional Chinese medicine, promotes viscera and remove blood stasis. Rhubarb is skilled in smoothening meridians, improving blood circulation which exhibits better efficacy on cerebral ischemic stroke. In this study, we aimed to analyze the underlying mechanisms of rhubarb...
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| description | Background. Rhubarb, a traditional Chinese medicine, promotes viscera and remove blood stasis. Rhubarb is skilled in smoothening meridians, improving blood circulation which exhibits better efficacy on cerebral ischemic stroke. In this study, we aimed to analyze the underlying mechanisms of rhubarb which treated rats of middle cerebral artery occlusion (MCAO) model according to an iTRAQ-based proteomics and bioinformatics analysis. 30 rats were randomly allocated into three groups including sham group (SG), model group (MG), and rhubarb group (RG). Rhubarb group was given a gavage of rhubarb decoction at dose of 3 g/kg and the remaining groups were prepared with normal saline by gavage. Rats from MG and RG were induced into MCAO model. The effects of rhubarb were estimated by Modified Neurological Severity Score (mNSS) and cerebral infarct volume. The brain tissues were measured via the quantitative proteomic approach of iTRAQ coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS). Furthermore, the bioinformatics analysis of overlapping differentially expression proteins (DEPs) was conducted by DAVID, KEGG, and Cytoscape. Specific selective DEPs were validated by Western blotting. Rats treated with rhubarb after MCAO showed a significant reduction on mNSS and cerebral infarct volume compared with MG. In MG versus SG and RG versus MG, we identified a total of 4578 proteins, of which 287 were DEPs. There were 76 overlapping DEPs between MG versus SG and RG versus MG. Through bioinformatics analysis, 14 associated pathways were searched including cGMP-PKG signaling pathway, tuberculosis, synaptic vesicle cycle, amyotrophic lateral sclerosis, long-term potentiation, and so on. 76 overlapping DEPs mainly involved synaptic vesicle cycling biological processes based on GO annotation. Further, the selective overlapping DEPs were verified at the protein level by using Western blotting. Our present study reveals that rhubarb highlights promising neuroprotective effect. Rhubarb exerts novel therapeutic action via modulating multiple proteins, targets, and pathways. |
| doi_str_mv | 10.1155/2018/6920213 |
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Rhubarb, a traditional Chinese medicine, promotes viscera and remove blood stasis. Rhubarb is skilled in smoothening meridians, improving blood circulation which exhibits better efficacy on cerebral ischemic stroke. In this study, we aimed to analyze the underlying mechanisms of rhubarb which treated rats of middle cerebral artery occlusion (MCAO) model according to an iTRAQ-based proteomics and bioinformatics analysis. 30 rats were randomly allocated into three groups including sham group (SG), model group (MG), and rhubarb group (RG). Rhubarb group was given a gavage of rhubarb decoction at dose of 3 g/kg and the remaining groups were prepared with normal saline by gavage. Rats from MG and RG were induced into MCAO model. The effects of rhubarb were estimated by Modified Neurological Severity Score (mNSS) and cerebral infarct volume. The brain tissues were measured via the quantitative proteomic approach of iTRAQ coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS). Furthermore, the bioinformatics analysis of overlapping differentially expression proteins (DEPs) was conducted by DAVID, KEGG, and Cytoscape. Specific selective DEPs were validated by Western blotting. Rats treated with rhubarb after MCAO showed a significant reduction on mNSS and cerebral infarct volume compared with MG. In MG versus SG and RG versus MG, we identified a total of 4578 proteins, of which 287 were DEPs. There were 76 overlapping DEPs between MG versus SG and RG versus MG. Through bioinformatics analysis, 14 associated pathways were searched including cGMP-PKG signaling pathway, tuberculosis, synaptic vesicle cycle, amyotrophic lateral sclerosis, long-term potentiation, and so on. 76 overlapping DEPs mainly involved synaptic vesicle cycling biological processes based on GO annotation. Further, the selective overlapping DEPs were verified at the protein level by using Western blotting. Our present study reveals that rhubarb highlights promising neuroprotective effect. Rhubarb exerts novel therapeutic action via modulating multiple proteins, targets, and pathways.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2018/6920213</identifier><identifier>PMID: 30112417</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Amyotrophic lateral sclerosis ; Animals ; Annotations ; Bioinformatics ; Biological activity ; Biomedical research ; Blood circulation ; Brain ; Brain Ischemia - drug therapy ; Cerebral blood flow ; Cerebral infarction ; Cyclic GMP ; Herbal medicine ; Ischemia ; Kinases ; Liquid chromatography ; Long-term potentiation ; Male ; Mass spectrometry ; Mass spectroscopy ; Medicine, Chinese Traditional ; Neuroprotection ; Occlusion ; Proteins ; Proteomics ; Rats ; Rats, Sprague-Dawley ; Rheum ; Rhubarb ; Rodents ; Signal transduction ; Stroke ; Stroke (Disease) ; Stroke - drug therapy ; Traditional Chinese medicine ; Tuberculosis ; Veins & arteries ; Viscera ; Western blotting</subject><ispartof>BioMed research international, 2018-01, Vol.2018 (2018), p.1-13</ispartof><rights>Copyright © 2018 Xiangping Lin et al.</rights><rights>COPYRIGHT 2018 John Wiley & Sons, Inc.</rights><rights>Copyright © 2018 Xiangping Lin et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2018 Xiangping Lin et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-717f246a4d34c3c0a2f580630cd5485e199125bf3a436b92bae8d1530fd1caf83</citedby><cites>FETCH-LOGICAL-c499t-717f246a4d34c3c0a2f580630cd5485e199125bf3a436b92bae8d1530fd1caf83</cites><orcidid>0000-0003-0447-3107 ; 0000-0002-4642-4314 ; 0000-0002-9622-533X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077657/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077657/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30112417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Jha, Sushil K.</contributor><contributor>Sushil K Jha</contributor><creatorcontrib>Tang, Tao</creatorcontrib><creatorcontrib>Wang, Yang</creatorcontrib><creatorcontrib>Cui, Hanjin</creatorcontrib><creatorcontrib>Zhong, Yuanyuan</creatorcontrib><creatorcontrib>He, Zehui</creatorcontrib><creatorcontrib>Li, Pengfei</creatorcontrib><creatorcontrib>Liu, Tao</creatorcontrib><creatorcontrib>Lin, Xiangping</creatorcontrib><creatorcontrib>Luo, Jiekun</creatorcontrib><title>iTRAQ-Based Proteomics Analysis Reveals the Effect of Rhubarb in Rats with Ischemic Stroke</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Background. Rhubarb, a traditional Chinese medicine, promotes viscera and remove blood stasis. Rhubarb is skilled in smoothening meridians, improving blood circulation which exhibits better efficacy on cerebral ischemic stroke. In this study, we aimed to analyze the underlying mechanisms of rhubarb which treated rats of middle cerebral artery occlusion (MCAO) model according to an iTRAQ-based proteomics and bioinformatics analysis. 30 rats were randomly allocated into three groups including sham group (SG), model group (MG), and rhubarb group (RG). Rhubarb group was given a gavage of rhubarb decoction at dose of 3 g/kg and the remaining groups were prepared with normal saline by gavage. Rats from MG and RG were induced into MCAO model. The effects of rhubarb were estimated by Modified Neurological Severity Score (mNSS) and cerebral infarct volume. The brain tissues were measured via the quantitative proteomic approach of iTRAQ coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS). Furthermore, the bioinformatics analysis of overlapping differentially expression proteins (DEPs) was conducted by DAVID, KEGG, and Cytoscape. Specific selective DEPs were validated by Western blotting. Rats treated with rhubarb after MCAO showed a significant reduction on mNSS and cerebral infarct volume compared with MG. In MG versus SG and RG versus MG, we identified a total of 4578 proteins, of which 287 were DEPs. There were 76 overlapping DEPs between MG versus SG and RG versus MG. Through bioinformatics analysis, 14 associated pathways were searched including cGMP-PKG signaling pathway, tuberculosis, synaptic vesicle cycle, amyotrophic lateral sclerosis, long-term potentiation, and so on. 76 overlapping DEPs mainly involved synaptic vesicle cycling biological processes based on GO annotation. Further, the selective overlapping DEPs were verified at the protein level by using Western blotting. Our present study reveals that rhubarb highlights promising neuroprotective effect. Rhubarb exerts novel therapeutic action via modulating multiple proteins, targets, and pathways.</description><subject>Amyotrophic lateral sclerosis</subject><subject>Animals</subject><subject>Annotations</subject><subject>Bioinformatics</subject><subject>Biological activity</subject><subject>Biomedical research</subject><subject>Blood circulation</subject><subject>Brain</subject><subject>Brain Ischemia - drug therapy</subject><subject>Cerebral blood flow</subject><subject>Cerebral infarction</subject><subject>Cyclic GMP</subject><subject>Herbal medicine</subject><subject>Ischemia</subject><subject>Kinases</subject><subject>Liquid chromatography</subject><subject>Long-term potentiation</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicine, Chinese Traditional</subject><subject>Neuroprotection</subject><subject>Occlusion</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rheum</subject><subject>Rhubarb</subject><subject>Rodents</subject><subject>Signal transduction</subject><subject>Stroke</subject><subject>Stroke (Disease)</subject><subject>Stroke - drug therapy</subject><subject>Traditional Chinese medicine</subject><subject>Tuberculosis</subject><subject>Veins & arteries</subject><subject>Viscera</subject><subject>Western blotting</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkk1v1DAQhiMEolXpjTOyxAUJQj3-SnJBWqpSKlUClnLhYjnOuHHJxq2dbdV_X0e7bIETvtiSn3lsz-uieAn0PYCUR4xCfaQaRhnwJ8U-4yBKBQKe7tac7xWHKV3RPGpQtFHPiz1OAZiAar_46S-Wi2_lR5OwI19jmDCsvE1kMZrhPvlElniLZkhk6pGcOId2IsGRZb9uTWyJH8nSTInc-aknZ8n2mKvJ9ymGX_iieOZyJR5u54Pix6eTi-PP5fmX07PjxXlpRdNMZQWVY0IZ0XFhuaWGOVlTxantpKglQtMAk63jRnDVNqw1WHcgOXUdWONqflB82Hiv1-0KO4vjFM2gr6NfmXivg_H6753R9_oy3GpFq0rJKgvebAUx3KwxTXrlk8VhMCOGddKM1g2rJKUz-vof9CqsY-7VTFW1ECL39ZG6NANqP7qQz7WzVC8Ug5pSxWSm3m0oG0NKEd3uykD1nK6e09XbdDP-6s9n7uDfWWbg7Qbo_diZO_-fOswMOvNIA8v_BPgDUUyzcA</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Tang, Tao</creator><creator>Wang, Yang</creator><creator>Cui, Hanjin</creator><creator>Zhong, Yuanyuan</creator><creator>He, Zehui</creator><creator>Li, Pengfei</creator><creator>Liu, Tao</creator><creator>Lin, Xiangping</creator><creator>Luo, Jiekun</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0447-3107</orcidid><orcidid>https://orcid.org/0000-0002-4642-4314</orcidid><orcidid>https://orcid.org/0000-0002-9622-533X</orcidid></search><sort><creationdate>20180101</creationdate><title>iTRAQ-Based Proteomics Analysis Reveals the Effect of Rhubarb in Rats with Ischemic Stroke</title><author>Tang, Tao ; Wang, Yang ; Cui, Hanjin ; Zhong, Yuanyuan ; He, Zehui ; Li, Pengfei ; Liu, Tao ; Lin, Xiangping ; Luo, Jiekun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-717f246a4d34c3c0a2f580630cd5485e199125bf3a436b92bae8d1530fd1caf83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amyotrophic lateral sclerosis</topic><topic>Animals</topic><topic>Annotations</topic><topic>Bioinformatics</topic><topic>Biological activity</topic><topic>Biomedical research</topic><topic>Blood circulation</topic><topic>Brain</topic><topic>Brain Ischemia - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Tao</au><au>Wang, Yang</au><au>Cui, Hanjin</au><au>Zhong, Yuanyuan</au><au>He, Zehui</au><au>Li, Pengfei</au><au>Liu, Tao</au><au>Lin, Xiangping</au><au>Luo, Jiekun</au><au>Jha, Sushil K.</au><au>Sushil K Jha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>iTRAQ-Based Proteomics Analysis Reveals the Effect of Rhubarb in Rats with Ischemic Stroke</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>2018</volume><issue>2018</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Background. Rhubarb, a traditional Chinese medicine, promotes viscera and remove blood stasis. Rhubarb is skilled in smoothening meridians, improving blood circulation which exhibits better efficacy on cerebral ischemic stroke. In this study, we aimed to analyze the underlying mechanisms of rhubarb which treated rats of middle cerebral artery occlusion (MCAO) model according to an iTRAQ-based proteomics and bioinformatics analysis. 30 rats were randomly allocated into three groups including sham group (SG), model group (MG), and rhubarb group (RG). Rhubarb group was given a gavage of rhubarb decoction at dose of 3 g/kg and the remaining groups were prepared with normal saline by gavage. Rats from MG and RG were induced into MCAO model. The effects of rhubarb were estimated by Modified Neurological Severity Score (mNSS) and cerebral infarct volume. The brain tissues were measured via the quantitative proteomic approach of iTRAQ coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS). Furthermore, the bioinformatics analysis of overlapping differentially expression proteins (DEPs) was conducted by DAVID, KEGG, and Cytoscape. Specific selective DEPs were validated by Western blotting. Rats treated with rhubarb after MCAO showed a significant reduction on mNSS and cerebral infarct volume compared with MG. In MG versus SG and RG versus MG, we identified a total of 4578 proteins, of which 287 were DEPs. There were 76 overlapping DEPs between MG versus SG and RG versus MG. Through bioinformatics analysis, 14 associated pathways were searched including cGMP-PKG signaling pathway, tuberculosis, synaptic vesicle cycle, amyotrophic lateral sclerosis, long-term potentiation, and so on. 76 overlapping DEPs mainly involved synaptic vesicle cycling biological processes based on GO annotation. Further, the selective overlapping DEPs were verified at the protein level by using Western blotting. Our present study reveals that rhubarb highlights promising neuroprotective effect. Rhubarb exerts novel therapeutic action via modulating multiple proteins, targets, and pathways.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>30112417</pmid><doi>10.1155/2018/6920213</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-0447-3107</orcidid><orcidid>https://orcid.org/0000-0002-4642-4314</orcidid><orcidid>https://orcid.org/0000-0002-9622-533X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amyotrophic lateral sclerosis Animals Annotations Bioinformatics Biological activity Biomedical research Blood circulation Brain Brain Ischemia - drug therapy Cerebral blood flow Cerebral infarction Cyclic GMP Herbal medicine Ischemia Kinases Liquid chromatography Long-term potentiation Male Mass spectrometry Mass spectroscopy Medicine, Chinese Traditional Neuroprotection Occlusion Proteins Proteomics Rats Rats, Sprague-Dawley Rheum Rhubarb Rodents Signal transduction Stroke Stroke (Disease) Stroke - drug therapy Traditional Chinese medicine Tuberculosis Veins & arteries Viscera Western blotting |
| title | iTRAQ-Based Proteomics Analysis Reveals the Effect of Rhubarb in Rats with Ischemic Stroke |
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