Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods

Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods

The aim of this study was to determine the presence of Wolbachia spp. and Spiroplasma spp. in natural populations of sand flies in Turkey by molecular methods. A total of 40 Phlebotomus specimens (19 female and 21 male) were used in this study. Genomic DNA from whole sand flies was isolated and Wolb...

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Veröffentlicht in:Scientific reports 2018-07, Vol.8 (1), p.10616-5, Article 10616
Hauptverfasser: Karatepe, Bilge, Aksoy, Serap, Karatepe, Mustafa
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45/23
45/77
631/1647/1513/2216
631/337/151
Deoxyribonucleic acid
DNA
Females
Humanities and Social Sciences
Infections
Males
multidisciplinary
Natural populations
Primers
Science
Science (multidisciplinary)
Spiroplasma
Wolbachia
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Aksoy, Serap
Karatepe, Mustafa
description The aim of this study was to determine the presence of Wolbachia spp. and Spiroplasma spp. in natural populations of sand flies in Turkey by molecular methods. A total of 40 Phlebotomus specimens (19 female and 21 male) were used in this study. Genomic DNA from whole sand flies was isolated and Wolbachia spp. infection prevalence was investigated by using Wolbachia gene specific primer sets ( wsp and GroEL) . In addition, the DNA were analyzed for the presence of Spiroplasma infections utilizing bacterium specific 16 S rDNA PCR-amplification primers. Results of this analysis showed a Wolbachia infection prevalence of 70% (28/40). There was no sex-bias in infection prevalence, being 76% (16/21) and 63% (12/19) in males and females, respectively. Analysis of Spiroplasma infections indicated that 26% (5/19) of female sand flies were positive for infection, while none of the screened males (0/21) were positive. Of the 40 sand fly samples, only 2 were found to be positive for both Wolbachia spp . and Spiroplasma spp . The present study demonstrates the presence of Wolbachia and Spiroplasma infections in the natural sand fly populations in Turkey. This is the first report on Spiroplasma infection in the sand flies from Turkey.
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A total of 40 Phlebotomus specimens (19 female and 21 male) were used in this study. Genomic DNA from whole sand flies was isolated and Wolbachia spp. infection prevalence was investigated by using Wolbachia gene specific primer sets ( wsp and GroEL) . In addition, the DNA were analyzed for the presence of Spiroplasma infections utilizing bacterium specific 16 S rDNA PCR-amplification primers. Results of this analysis showed a Wolbachia infection prevalence of 70% (28/40). There was no sex-bias in infection prevalence, being 76% (16/21) and 63% (12/19) in males and females, respectively. Analysis of Spiroplasma infections indicated that 26% (5/19) of female sand flies were positive for infection, while none of the screened males (0/21) were positive. Of the 40 sand fly samples, only 2 were found to be positive for both Wolbachia spp . and Spiroplasma spp . The present study demonstrates the presence of Wolbachia and Spiroplasma infections in the natural sand fly populations in Turkey. 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A total of 40 Phlebotomus specimens (19 female and 21 male) were used in this study. Genomic DNA from whole sand flies was isolated and Wolbachia spp. infection prevalence was investigated by using Wolbachia gene specific primer sets ( wsp and GroEL) . In addition, the DNA were analyzed for the presence of Spiroplasma infections utilizing bacterium specific 16 S rDNA PCR-amplification primers. Results of this analysis showed a Wolbachia infection prevalence of 70% (28/40). There was no sex-bias in infection prevalence, being 76% (16/21) and 63% (12/19) in males and females, respectively. Analysis of Spiroplasma infections indicated that 26% (5/19) of female sand flies were positive for infection, while none of the screened males (0/21) were positive. Of the 40 sand fly samples, only 2 were found to be positive for both Wolbachia spp . and Spiroplasma spp . The present study demonstrates the presence of Wolbachia and Spiroplasma infections in the natural sand fly populations in Turkey. 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subjects 45/22
45/23
45/77
631/1647/1513/2216
631/337/151
Deoxyribonucleic acid
DNA
Females
Humanities and Social Sciences
Infections
Males
multidisciplinary
Natural populations
Primers
Science
Science (multidisciplinary)
Spiroplasma
Wolbachia
title Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods
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