Genetic code expansion and live cell imaging reveal that Thr-308 phosphorylation is irreplaceable and sufficient for Akt1 activity

The proto-oncogene Akt/protein kinase B (PKB) is a pivotal signal transducer for growth and survival. Growth factor stimulation leads to Akt phosphorylation at two regulatory sites (Thr-308 and Ser-473), acutely activating Akt signaling. Delineating the exact role of each regulatory site is, however...

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Veröffentlicht in:	The Journal of biological chemistry 2018-07, Vol.293 (27), p.10744-10756
Hauptverfasser:	Balasuriya, Nileeka, Kunkel, Maya T., Liu, Xuguang, Biggar, Kyle K., Li, Shawn S.-C., Newton, Alexandra C., O'Donoghue, Patrick
Format:	Artikel
Sprache:	eng
Schlagworte:	activation loop Akt/protein kinase B (PKB) aminoacyl tRNA synthetase cell biology cell signaling Cells, Cultured Crystallography, X-Ray enzyme Enzymology Genetic Code genetic code expansion Humans hydrophobic motif Molecular Imaging - methods Mutation phosphomimetics Phosphorylation phosphoseryl-tRNA synthetase (SepRS) Protein Conformation protein phosphorylation Proto-Oncogene Proteins c-akt - chemistry Proto-Oncogene Proteins c-akt - genetics Proto-Oncogene Proteins c-akt - metabolism Serine - chemistry Serine - genetics Serine - metabolism Threonine - chemistry Threonine - genetics Threonine - metabolism transfer RNA (tRNA) tRNASep
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