Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as pe...
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| creator | Dulbecco, Andrea B. Moriconi, Débora E. Calderón-Fernández, Gustavo M. Lynn, Soledad McCarthy, Andrés Roca-Acevedo, Gonzalo Salamanca-Moreno, Jhon A. Juárez, M. Patricia Pedrini, Nicolás |
| description | Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (
CYP
) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded
CYP3093A
and
CYP4EM
subfamilies in the Chagas disease vectors
Rhodnius prolixus
and
Triatoma infestans
. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant
T
.
infestans
nymphs (
CYP3093A11
and
CYP4EM10
) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two
CYP
genes do not fully explain the resistance observed in
T
.
infestans
. |
| doi_str_mv | 10.1038/s41598-018-28475-x |
| format | Article |
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CYP
) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded
CYP3093A
and
CYP4EM
subfamilies in the Chagas disease vectors
Rhodnius prolixus
and
Triatoma infestans
. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant
T
.
infestans
nymphs (
CYP3093A11
and
CYP4EM10
) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two
CYP
genes do not fully explain the resistance observed in
T
.
infestans
.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-28475-x</identifier><identifier>PMID: 29976934</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/77 ; 38/89 ; 45/90 ; 631/208/199 ; 631/337/505 ; Amino acids ; Chagas disease ; Cytochrome ; Cytochrome P450 ; Deltamethrin ; Detoxification ; Fat body ; Genomes ; Humanities and Social Sciences ; Insecticides ; Insects ; Integument ; Metabolism ; multidisciplinary ; Science ; Science (multidisciplinary) ; Triatoma infestans ; Vector-borne diseases</subject><ispartof>Scientific reports, 2018-07, Vol.8 (1), p.10177-12, Article 10177</ispartof><rights>The Author(s) 2018</rights><rights>Copyright Nature Publishing Group Jul 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-191ef475cfc23bbbc1c595391c6dc8e402f7663177da23c7fbf1f8853e51b203</citedby><cites>FETCH-LOGICAL-c478t-191ef475cfc23bbbc1c595391c6dc8e402f7663177da23c7fbf1f8853e51b203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033900/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033900/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29976934$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dulbecco, Andrea B.</creatorcontrib><creatorcontrib>Moriconi, Débora E.</creatorcontrib><creatorcontrib>Calderón-Fernández, Gustavo M.</creatorcontrib><creatorcontrib>Lynn, Soledad</creatorcontrib><creatorcontrib>McCarthy, Andrés</creatorcontrib><creatorcontrib>Roca-Acevedo, Gonzalo</creatorcontrib><creatorcontrib>Salamanca-Moreno, Jhon A.</creatorcontrib><creatorcontrib>Juárez, M. Patricia</creatorcontrib><creatorcontrib>Pedrini, Nicolás</creatorcontrib><title>Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (
CYP
) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded
CYP3093A
and
CYP4EM
subfamilies in the Chagas disease vectors
Rhodnius prolixus
and
Triatoma infestans
. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant
T
.
infestans
nymphs (
CYP3093A11
and
CYP4EM10
) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two
CYP
genes do not fully explain the resistance observed in
T
.
infestans
.</description><subject>38/77</subject><subject>38/89</subject><subject>45/90</subject><subject>631/208/199</subject><subject>631/337/505</subject><subject>Amino acids</subject><subject>Chagas disease</subject><subject>Cytochrome</subject><subject>Cytochrome P450</subject><subject>Deltamethrin</subject><subject>Detoxification</subject><subject>Fat body</subject><subject>Genomes</subject><subject>Humanities and Social Sciences</subject><subject>Insecticides</subject><subject>Insects</subject><subject>Integument</subject><subject>Metabolism</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Triatoma infestans</subject><subject>Vector-borne diseases</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNp9UctuFDEQtBAoiZL8QA7IEucBv-bhCxJa8YgUiRz2wsnyeNqzjnbswfbC5n_4UDxMCOESX2xXV1eXuhC6ouQtJbx7lwStZVcR2lWsE21dHV-gM0ZEXTHO2Msn71N0mdIdKadmUlB5gk6ZlG0juThDv659hvEwgc948-0Wj-Ah4WBx3gHe6zhCygsYJqh-ugGwuc_B7GL541tREwzHWfvkgk_YeZyj0zlMbhGZdczOuFlnWEoD5HB01hmdC3tF9llPkHfR-SpCcinrYmO7auhCsbBA6QK9snqf4PLhPkfbTx-3my_VzdfP15sPN5URbZcrKinYsgpjDeN93xtqallzSU0zmA4EYbZtGk7bdtCMm9b2ltquqznUtGeEn6P3q-x86CcYTNlJ1Hs1RzfpeK-Cdur_inc7NYYfqiGcS7IIvHkQiOH7oXhXd-EQfbGsGGlEKzgTvLDYyjIxpBTBPk6gRC3ZqjVbVbJVf7JVx9L0-qm3x5a_SRYCXwmplPwI8d_sZ2R_A6DutXU</recordid><startdate>20180705</startdate><enddate>20180705</enddate><creator>Dulbecco, Andrea B.</creator><creator>Moriconi, Débora E.</creator><creator>Calderón-Fernández, Gustavo M.</creator><creator>Lynn, Soledad</creator><creator>McCarthy, Andrés</creator><creator>Roca-Acevedo, Gonzalo</creator><creator>Salamanca-Moreno, Jhon A.</creator><creator>Juárez, M. Patricia</creator><creator>Pedrini, Nicolás</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20180705</creationdate><title>Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans</title><author>Dulbecco, Andrea B. ; Moriconi, Débora E. ; Calderón-Fernández, Gustavo M. ; Lynn, Soledad ; McCarthy, Andrés ; Roca-Acevedo, Gonzalo ; Salamanca-Moreno, Jhon A. ; Juárez, M. Patricia ; Pedrini, Nicolás</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-191ef475cfc23bbbc1c595391c6dc8e402f7663177da23c7fbf1f8853e51b203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>38/77</topic><topic>38/89</topic><topic>45/90</topic><topic>631/208/199</topic><topic>631/337/505</topic><topic>Amino acids</topic><topic>Chagas disease</topic><topic>Cytochrome</topic><topic>Cytochrome P450</topic><topic>Deltamethrin</topic><topic>Detoxification</topic><topic>Fat body</topic><topic>Genomes</topic><topic>Humanities and Social Sciences</topic><topic>Insecticides</topic><topic>Insects</topic><topic>Integument</topic><topic>Metabolism</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Triatoma infestans</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dulbecco, Andrea B.</creatorcontrib><creatorcontrib>Moriconi, Débora E.</creatorcontrib><creatorcontrib>Calderón-Fernández, Gustavo M.</creatorcontrib><creatorcontrib>Lynn, Soledad</creatorcontrib><creatorcontrib>McCarthy, Andrés</creatorcontrib><creatorcontrib>Roca-Acevedo, Gonzalo</creatorcontrib><creatorcontrib>Salamanca-Moreno, Jhon A.</creatorcontrib><creatorcontrib>Juárez, M. Patricia</creatorcontrib><creatorcontrib>Pedrini, Nicolás</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dulbecco, Andrea B.</au><au>Moriconi, Débora E.</au><au>Calderón-Fernández, Gustavo M.</au><au>Lynn, Soledad</au><au>McCarthy, Andrés</au><au>Roca-Acevedo, Gonzalo</au><au>Salamanca-Moreno, Jhon A.</au><au>Juárez, M. Patricia</au><au>Pedrini, Nicolás</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-07-05</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>10177</spage><epage>12</epage><pages>10177-12</pages><artnum>10177</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (
CYP
) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded
CYP3093A
and
CYP4EM
subfamilies in the Chagas disease vectors
Rhodnius prolixus
and
Triatoma infestans
. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant
T
.
infestans
nymphs (
CYP3093A11
and
CYP4EM10
) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two
CYP
genes do not fully explain the resistance observed in
T
.
infestans
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29976934</pmid><doi>10.1038/s41598-018-28475-x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 38/77 38/89 45/90 631/208/199 631/337/505 Amino acids Chagas disease Cytochrome Cytochrome P450 Deltamethrin Detoxification Fat body Genomes Humanities and Social Sciences Insecticides Insects Integument Metabolism multidisciplinary Science Science (multidisciplinary) Triatoma infestans Vector-borne diseases |
| title | Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans |
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