Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis
The cellular complexity of human brain development has been intensively investigated, although a regional characterization of the entire human cerebral cortex based on single-cell transcriptome analysis has not been reported. Here, we performed RNA-seq on over 4,000 individual cells from 22 brain re...
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| Veröffentlicht in: | Cell research 2018-07, Vol.28 (7), p.730-745 |
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| creator | Fan, Xiaoying Dong, Ji Zhong, Suijuan Wei, Yuan Wu, Qian Yan, Liying Yong, Jun Sun, Le Wang, Xiaoye Zhao, Yangyu Wang, Wei Yan, Jie Wang, Xiaoqun Qiao, Jie Tang, Fuchou |
| description | The cellular complexity of human brain development has been intensively investigated, although a regional characterization of the entire human cerebral cortex based on single-cell transcriptome analysis has not been reported. Here, we performed RNA-seq on over 4,000 individual cells from 22 brain regions of human mid-gestation embryos. We identified 29 cell sub-clusters, which showed different proportions in each region and the pons showed especially high percentage of astrocytes. Embryonic neurons were not as diverse as adult neurons, although they possessed important features of their destinies in adults. Neuron development was unsynchronized in the cerebral cortex, as dorsal regions appeared to be more mature than ventral regions at this stage. Region-specific genes were comprehensively identified in each neuronal sub-cluster, and a large proportion of these genes were neural disease related. Our results present a systematic landscape of the regionalized gene expression and neuron maturation of the human cerebral cortex. |
| doi_str_mv | 10.1038/s41422-018-0053-3 |
| format | Article |
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Here, we performed RNA-seq on over 4,000 individual cells from 22 brain regions of human mid-gestation embryos. We identified 29 cell sub-clusters, which showed different proportions in each region and the pons showed especially high percentage of astrocytes. Embryonic neurons were not as diverse as adult neurons, although they possessed important features of their destinies in adults. Neuron development was unsynchronized in the cerebral cortex, as dorsal regions appeared to be more mature than ventral regions at this stage. Region-specific genes were comprehensively identified in each neuronal sub-cluster, and a large proportion of these genes were neural disease related. Our results present a systematic landscape of the regionalized gene expression and neuron maturation of the human cerebral cortex.</description><identifier>ISSN: 1001-0602</identifier><identifier>EISSN: 1748-7838</identifier><identifier>DOI: 10.1038/s41422-018-0053-3</identifier><identifier>PMID: 29867213</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38 ; 38/39 ; 38/77 ; 38/91 ; 631/136 ; 631/337 ; 82/51 ; Adults ; Astrocytes ; Biomedical and Life Sciences ; Brain ; Cell Biology ; Cerebral cortex ; Embryos ; Gene expression ; Genes ; Gestation ; Life Sciences ; Maturation ; Neurons ; Pons ; Regional development ; Ribonucleic acid ; RNA</subject><ispartof>Cell research, 2018-07, Vol.28 (7), p.730-745</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>IBCB, SIBS, CAS 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-c7e3b82d1b3b979d9b41468fc0b13a56dbb62f2cf74450893f612ad447f14a653</citedby><cites>FETCH-LOGICAL-c536t-c7e3b82d1b3b979d9b41468fc0b13a56dbb62f2cf74450893f612ad447f14a653</cites><orcidid>0000-0003-2126-1376 ; 0000-0002-8625-7717</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028726/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028726/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,41467,42536,51298,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29867213$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fan, Xiaoying</creatorcontrib><creatorcontrib>Dong, Ji</creatorcontrib><creatorcontrib>Zhong, Suijuan</creatorcontrib><creatorcontrib>Wei, Yuan</creatorcontrib><creatorcontrib>Wu, Qian</creatorcontrib><creatorcontrib>Yan, Liying</creatorcontrib><creatorcontrib>Yong, Jun</creatorcontrib><creatorcontrib>Sun, Le</creatorcontrib><creatorcontrib>Wang, Xiaoye</creatorcontrib><creatorcontrib>Zhao, Yangyu</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Yan, Jie</creatorcontrib><creatorcontrib>Wang, Xiaoqun</creatorcontrib><creatorcontrib>Qiao, Jie</creatorcontrib><creatorcontrib>Tang, Fuchou</creatorcontrib><title>Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis</title><title>Cell research</title><addtitle>Cell Res</addtitle><addtitle>Cell Res</addtitle><description>The cellular complexity of human brain development has been intensively investigated, although a regional characterization of the entire human cerebral cortex based on single-cell transcriptome analysis has not been reported. Here, we performed RNA-seq on over 4,000 individual cells from 22 brain regions of human mid-gestation embryos. We identified 29 cell sub-clusters, which showed different proportions in each region and the pons showed especially high percentage of astrocytes. Embryonic neurons were not as diverse as adult neurons, although they possessed important features of their destinies in adults. Neuron development was unsynchronized in the cerebral cortex, as dorsal regions appeared to be more mature than ventral regions at this stage. Region-specific genes were comprehensively identified in each neuronal sub-cluster, and a large proportion of these genes were neural disease related. Our results present a systematic landscape of the regionalized gene expression and neuron maturation of the human cerebral cortex.</description><subject>38</subject><subject>38/39</subject><subject>38/77</subject><subject>38/91</subject><subject>631/136</subject><subject>631/337</subject><subject>82/51</subject><subject>Adults</subject><subject>Astrocytes</subject><subject>Biomedical and Life Sciences</subject><subject>Brain</subject><subject>Cell Biology</subject><subject>Cerebral cortex</subject><subject>Embryos</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Gestation</subject><subject>Life Sciences</subject><subject>Maturation</subject><subject>Neurons</subject><subject>Pons</subject><subject>Regional development</subject><subject>Ribonucleic 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Xiaoye</au><au>Zhao, Yangyu</au><au>Wang, Wei</au><au>Yan, Jie</au><au>Wang, Xiaoqun</au><au>Qiao, Jie</au><au>Tang, Fuchou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis</atitle><jtitle>Cell research</jtitle><stitle>Cell Res</stitle><addtitle>Cell Res</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>28</volume><issue>7</issue><spage>730</spage><epage>745</epage><pages>730-745</pages><issn>1001-0602</issn><eissn>1748-7838</eissn><abstract>The cellular complexity of human brain development has been intensively investigated, although a regional characterization of the entire human cerebral cortex based on single-cell transcriptome analysis has not been reported. Here, we performed RNA-seq on over 4,000 individual cells from 22 brain regions of human mid-gestation embryos. We identified 29 cell sub-clusters, which showed different proportions in each region and the pons showed especially high percentage of astrocytes. Embryonic neurons were not as diverse as adult neurons, although they possessed important features of their destinies in adults. Neuron development was unsynchronized in the cerebral cortex, as dorsal regions appeared to be more mature than ventral regions at this stage. Region-specific genes were comprehensively identified in each neuronal sub-cluster, and a large proportion of these genes were neural disease related. 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| subjects | 38 38/39 38/77 38/91 631/136 631/337 82/51 Adults Astrocytes Biomedical and Life Sciences Brain Cell Biology Cerebral cortex Embryos Gene expression Genes Gestation Life Sciences Maturation Neurons Pons Regional development Ribonucleic acid RNA |
| title | Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis |
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