Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus)

Environmental DNA (eDNA) analysis is a rapid, cost‐effective, non‐invasive biodiversity monitoring tool which utilises DNA left behind in the environment by organisms for species detection. The method is used as a species‐specific survey tool for rare or invasive species across a broad range of ecos...

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Veröffentlicht in:	Ecology and evolution 2018-06, Vol.8 (12), p.6330-6341
Hauptverfasser:	Harper, Lynsey R., Lawson Handley, Lori, Hahn, Christoph, Boonham, Neil, Rees, Helen C., Gough, Kevin C., Lewis, Erin, Adams, Ian P., Brotherton, Peter, Phillips, Susanna, Hänfling, Bernd
Format:	Artikel
Sprache:	eng
Schlagworte:	Amphibians Biodiversity Communities Conservation Cost analysis Deoxyribonucleic acid DNA Environmental DNA Glyptemys insculpta great crested newt high‐throughput sequencing Introduced species Invasive species metabarcoding Next-generation sequencing Original Research Polls & surveys Ponds Rare species real‐time quantitative PCR Sensitivity analysis Sequences Triturus cristatus Wildlife conservation
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