Synchrotron- and focal plane array-based Fourier-transform infrared spectroscopy differentiates the basalis and functionalis epithelial endometrial regions and identifies putative stem cell regions of human endometrial glands
The cyclical process of regeneration of the endometrium suggests that it may contain a cell population that can provide daughter cells with high proliferative potential. These cell lineages are clinically significant as they may represent clonogenic cells that may also be involved in tumourigenesis...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2018-07, Vol.410 (18), p.4541-4554 |
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| creator | Theophilou, Georgios Morais, Camilo L. M. Halliwell, Diane E. Lima, Kássio M. G. Drury, Josephine Martin-Hirsch, Pierre L. Stringfellow, Helen F. Hapangama, Dharani K. Martin, Francis L. |
| description | The cyclical process of regeneration of the endometrium suggests that it may contain a cell population that can provide daughter cells with high proliferative potential. These cell lineages are clinically significant as they may represent clonogenic cells that may also be involved in tumourigenesis as well as endometriotic lesion development. To determine whether the putative stem cell location within human uterine tissue can be derived using vibrational spectroscopy techniques, normal endometrial tissue was interrogated by two spectroscopic techniques. Paraffin-embedded uterine tissues containing endometrial glands were sectioned to 10-μm-thick parallel tissue sections and were floated onto BaF
2
slides for synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy and globar focal plane array-based FTIR spectroscopy. Different spectral characteristics were identified depending on the location of the glands examined. The resulting infrared spectra were subjected to multivariate analysis to determine associated biophysical differences along the length of longitudinal and crosscut gland sections. Comparison of the epithelial cellular layer of transverse gland sections revealed alterations indicating the presence of putative transient-amplifying-like cells in the basalis and mitotic cells in the functionalis. SR-FTIR microspectroscopy of the base of the endometrial glands identified the location where putative stem cells may reside at the same time pointing towards ν
s
PO
2
−
in DNA and RNA, nucleic acids and amide I and II vibrations as major discriminating factors. This study supports the view that vibration spectroscopy technologies are a powerful adjunct to our understanding of the stem cell biology of endometrial tissue.
Graphical abstract
ᅟ |
| doi_str_mv | 10.1007/s00216-018-1111-x |
| format | Article |
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2
slides for synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy and globar focal plane array-based FTIR spectroscopy. Different spectral characteristics were identified depending on the location of the glands examined. The resulting infrared spectra were subjected to multivariate analysis to determine associated biophysical differences along the length of longitudinal and crosscut gland sections. Comparison of the epithelial cellular layer of transverse gland sections revealed alterations indicating the presence of putative transient-amplifying-like cells in the basalis and mitotic cells in the functionalis. SR-FTIR microspectroscopy of the base of the endometrial glands identified the location where putative stem cells may reside at the same time pointing towards ν
s
PO
2
−
in DNA and RNA, nucleic acids and amide I and II vibrations as major discriminating factors. This study supports the view that vibration spectroscopy technologies are a powerful adjunct to our understanding of the stem cell biology of endometrial tissue.
Graphical abstract
ᅟ</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-018-1111-x</identifier><identifier>PMID: 29740671</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Barium fluorides ; Biochemistry ; carcinogenesis ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Deoxyribonucleic acid ; DNA ; Endometrium ; epithelium ; Focal plane devices ; Food Science ; Fourier transform infrared spectroscopy ; Fourier transforms ; Glands ; humans ; Infrared analysis ; Infrared spectra ; Infrared spectroscopy ; Laboratory Medicine ; Methods ; mitosis ; Monitoring/Environmental Analysis ; Multivariate analysis ; Nucleic acids ; Paraffin ; Physiological aspects ; Regeneration ; Research Paper ; Ribonucleic acid ; RNA ; spectral analysis ; Spectrum analysis ; Stem cells ; Synchrotron radiation ; Tissues ; Uterus ; vibration ; Vibrations</subject><ispartof>Analytical and bioanalytical chemistry, 2018-07, Vol.410 (18), p.4541-4554</ispartof><rights>The Author(s) 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c568t-daaf931e2193a87d1b255e01bd54a2ccbbd68edf21f3532ba41f2dfb27e1c8c3</citedby><cites>FETCH-LOGICAL-c568t-daaf931e2193a87d1b255e01bd54a2ccbbd68edf21f3532ba41f2dfb27e1c8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-018-1111-x$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-018-1111-x$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29740671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Theophilou, Georgios</creatorcontrib><creatorcontrib>Morais, Camilo L. M.</creatorcontrib><creatorcontrib>Halliwell, Diane E.</creatorcontrib><creatorcontrib>Lima, Kássio M. G.</creatorcontrib><creatorcontrib>Drury, Josephine</creatorcontrib><creatorcontrib>Martin-Hirsch, Pierre L.</creatorcontrib><creatorcontrib>Stringfellow, Helen F.</creatorcontrib><creatorcontrib>Hapangama, Dharani K.</creatorcontrib><creatorcontrib>Martin, Francis L.</creatorcontrib><title>Synchrotron- and focal plane array-based Fourier-transform infrared spectroscopy differentiates the basalis and functionalis epithelial endometrial regions and identifies putative stem cell regions of human endometrial glands</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>The cyclical process of regeneration of the endometrium suggests that it may contain a cell population that can provide daughter cells with high proliferative potential. These cell lineages are clinically significant as they may represent clonogenic cells that may also be involved in tumourigenesis as well as endometriotic lesion development. To determine whether the putative stem cell location within human uterine tissue can be derived using vibrational spectroscopy techniques, normal endometrial tissue was interrogated by two spectroscopic techniques. Paraffin-embedded uterine tissues containing endometrial glands were sectioned to 10-μm-thick parallel tissue sections and were floated onto BaF
2
slides for synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy and globar focal plane array-based FTIR spectroscopy. Different spectral characteristics were identified depending on the location of the glands examined. The resulting infrared spectra were subjected to multivariate analysis to determine associated biophysical differences along the length of longitudinal and crosscut gland sections. Comparison of the epithelial cellular layer of transverse gland sections revealed alterations indicating the presence of putative transient-amplifying-like cells in the basalis and mitotic cells in the functionalis. SR-FTIR microspectroscopy of the base of the endometrial glands identified the location where putative stem cells may reside at the same time pointing towards ν
s
PO
2
−
in DNA and RNA, nucleic acids and amide I and II vibrations as major discriminating factors. This study supports the view that vibration spectroscopy technologies are a powerful adjunct to our understanding of the stem cell biology of endometrial tissue.
Graphical abstract
ᅟ</description><subject>Analytical Chemistry</subject><subject>Barium fluorides</subject><subject>Biochemistry</subject><subject>carcinogenesis</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Endometrium</subject><subject>epithelium</subject><subject>Focal plane devices</subject><subject>Food Science</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Fourier transforms</subject><subject>Glands</subject><subject>humans</subject><subject>Infrared analysis</subject><subject>Infrared spectra</subject><subject>Infrared spectroscopy</subject><subject>Laboratory Medicine</subject><subject>Methods</subject><subject>mitosis</subject><subject>Monitoring/Environmental Analysis</subject><subject>Multivariate analysis</subject><subject>Nucleic acids</subject><subject>Paraffin</subject><subject>Physiological aspects</subject><subject>Regeneration</subject><subject>Research Paper</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>spectral analysis</subject><subject>Spectrum analysis</subject><subject>Stem cells</subject><subject>Synchrotron radiation</subject><subject>Tissues</subject><subject>Uterus</subject><subject>vibration</subject><subject>Vibrations</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNqNksFu1DAQhiMEoqXwAFyQJS5cAraTONkLUlVRQKrEgd4txx7vukrsYDtV93F5EyZN2aVISOQSO_P5i-35i-I1o-8Zpe2HRClnoqSsKxk-5d2T4pQJnHHR0KeHcc1Pihcp3VDKmo6J58UJ37Q1FS07LX5-33u9iyHH4EuivCE2aDWQaVAeiIpR7cteJTDkMszRQSxzVD7ZEEfivI0qYilNoFGQdJj2xDhrIYLPTmVIJO-AoEANLq362evsgr__AJPD-uDwh-BNGCHHZRxhi8TKO7OorEPVNGeV3S2QlGEkGoYjGSzZzaPyjzRbPINJL4tnVg0JXj28z4rry0_XF1_Kq2-fv16cX5W6EV0ujVJ2UzHgbFOprjWs500DlPWmqRXXuu-N6MBYzmzVVLxXNbPc2J63wHSnq7Pi46qd5n4Eo3HXUQ1yim5UcS-DcvJxxbud3IZbKbCHtehQ8O5BEMOPGVKWo0vLIbERYU6SM8qqjtf1f6C0Em0naiEQffsXeoN9xNu_p5pN3TDOj9RWDSCxr5gHpRepPG9qzMym5Q1SbKU09jpFsIfTMSqXQMo1kBIDKZdAyjtc8-bPazms-J1ABPgKJCz5LcTjBv9t_QWR4vPY</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Theophilou, Georgios</creator><creator>Morais, Camilo L. 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M.</au><au>Halliwell, Diane E.</au><au>Lima, Kássio M. G.</au><au>Drury, Josephine</au><au>Martin-Hirsch, Pierre L.</au><au>Stringfellow, Helen F.</au><au>Hapangama, Dharani K.</au><au>Martin, Francis L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synchrotron- and focal plane array-based Fourier-transform infrared spectroscopy differentiates the basalis and functionalis epithelial endometrial regions and identifies putative stem cell regions of human endometrial glands</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>410</volume><issue>18</issue><spage>4541</spage><epage>4554</epage><pages>4541-4554</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>The cyclical process of regeneration of the endometrium suggests that it may contain a cell population that can provide daughter cells with high proliferative potential. These cell lineages are clinically significant as they may represent clonogenic cells that may also be involved in tumourigenesis as well as endometriotic lesion development. To determine whether the putative stem cell location within human uterine tissue can be derived using vibrational spectroscopy techniques, normal endometrial tissue was interrogated by two spectroscopic techniques. Paraffin-embedded uterine tissues containing endometrial glands were sectioned to 10-μm-thick parallel tissue sections and were floated onto BaF
2
slides for synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy and globar focal plane array-based FTIR spectroscopy. Different spectral characteristics were identified depending on the location of the glands examined. The resulting infrared spectra were subjected to multivariate analysis to determine associated biophysical differences along the length of longitudinal and crosscut gland sections. Comparison of the epithelial cellular layer of transverse gland sections revealed alterations indicating the presence of putative transient-amplifying-like cells in the basalis and mitotic cells in the functionalis. SR-FTIR microspectroscopy of the base of the endometrial glands identified the location where putative stem cells may reside at the same time pointing towards ν
s
PO
2
−
in DNA and RNA, nucleic acids and amide I and II vibrations as major discriminating factors. This study supports the view that vibration spectroscopy technologies are a powerful adjunct to our understanding of the stem cell biology of endometrial tissue.
Graphical abstract
ᅟ</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29740671</pmid><doi>10.1007/s00216-018-1111-x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2018-07, Vol.410 (18), p.4541-4554 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Analytical Chemistry Barium fluorides Biochemistry carcinogenesis Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Deoxyribonucleic acid DNA Endometrium epithelium Focal plane devices Food Science Fourier transform infrared spectroscopy Fourier transforms Glands humans Infrared analysis Infrared spectra Infrared spectroscopy Laboratory Medicine Methods mitosis Monitoring/Environmental Analysis Multivariate analysis Nucleic acids Paraffin Physiological aspects Regeneration Research Paper Ribonucleic acid RNA spectral analysis Spectrum analysis Stem cells Synchrotron radiation Tissues Uterus vibration Vibrations |
| title | Synchrotron- and focal plane array-based Fourier-transform infrared spectroscopy differentiates the basalis and functionalis epithelial endometrial regions and identifies putative stem cell regions of human endometrial glands |
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