Cybrid Models of Pathological Cell Processes in Different Diseases
Modelling of pathological processes in cells is one of the most sought-after technologies of the 21st century. Using models of such processes may help to study the pathogenetic mechanisms of various diseases. The aim of the present study was to analyse the literature, dedicated to obtaining and inve...
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| Veröffentlicht in: | Oxidative medicine and cellular longevity 2018-01, Vol.2018 (2018), p.1-6 |
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| creator | Sobenin, Igor A. Postnov, Anton Y. Melnichenko, Alexandra A. Galitsyna, Elena V. Ryzhkova, Anastasia I. Sinyov, Vasily V. Sazonova, Margarita A. Orekhov, Alexander N. |
| description | Modelling of pathological processes in cells is one of the most sought-after technologies of the 21st century. Using models of such processes may help to study the pathogenetic mechanisms of various diseases. The aim of the present study was to analyse the literature, dedicated to obtaining and investigating cybrid models. Besides, the possibility of modeling pathological processes in cells and treatment of different diseases using the models was evaluated. Methods of obtaining Rho0 cell cultures showed that, during their creation, mainly a standard technique, based on the use of mtDNA replication inhibitors (ethidium bromide), was applied. Cybrid lines were usually obtained by PEG fusion. Most frequently, platelets acted as donors of mitochondria. According to the analysis of the literature data, cybrid cell cultures can be modeled to study the dysfunction of the mitochondrial genome and molecular cellular pathological processes. Such models can be very promising for the development of therapeutic approaches to the treatment of various human diseases. |
| doi_str_mv | 10.1155/2018/4647214 |
| format | Article |
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Using models of such processes may help to study the pathogenetic mechanisms of various diseases. The aim of the present study was to analyse the literature, dedicated to obtaining and investigating cybrid models. Besides, the possibility of modeling pathological processes in cells and treatment of different diseases using the models was evaluated. Methods of obtaining Rho0 cell cultures showed that, during their creation, mainly a standard technique, based on the use of mtDNA replication inhibitors (ethidium bromide), was applied. Cybrid lines were usually obtained by PEG fusion. Most frequently, platelets acted as donors of mitochondria. According to the analysis of the literature data, cybrid cell cultures can be modeled to study the dysfunction of the mitochondrial genome and molecular cellular pathological processes. Such models can be very promising for the development of therapeutic approaches to the treatment of various human diseases.</description><identifier>ISSN: 1942-0900</identifier><identifier>EISSN: 1942-0994</identifier><identifier>DOI: 10.1155/2018/4647214</identifier><identifier>PMID: 29983856</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Cell culture ; Deoxyribonucleic acid ; Disease ; DNA ; Genes ; Genomes ; Genomics ; Medical research ; Medicine, Experimental ; Mitochondria ; Mitochondrial DNA ; Mutation ; Review</subject><ispartof>Oxidative medicine and cellular longevity, 2018-01, Vol.2018 (2018), p.1-6</ispartof><rights>Copyright © 2018 Margarita A. Sazonova et al.</rights><rights>COPYRIGHT 2018 John Wiley & Sons, Inc.</rights><rights>Copyright © 2018 Margarita A. Sazonova et al.; This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2018 Margarita A. Sazonova et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-dfdad449a630e63e379c72864bb2d61efeb086e1603ae22882532e6c4d2567f83</citedby><cites>FETCH-LOGICAL-c499t-dfdad449a630e63e379c72864bb2d61efeb086e1603ae22882532e6c4d2567f83</cites><orcidid>0000-0002-8610-4593 ; 0000-0002-8838-7750 ; 0000-0002-6495-1628 ; 0000-0002-2501-7269 ; 0000-0001-5105-5763 ; 0000-0003-0978-6444</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015674/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015674/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29983856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ramana, Kota V.</contributor><contributor>Kota V Ramana</contributor><creatorcontrib>Sobenin, Igor A.</creatorcontrib><creatorcontrib>Postnov, Anton Y.</creatorcontrib><creatorcontrib>Melnichenko, Alexandra A.</creatorcontrib><creatorcontrib>Galitsyna, Elena V.</creatorcontrib><creatorcontrib>Ryzhkova, Anastasia I.</creatorcontrib><creatorcontrib>Sinyov, Vasily V.</creatorcontrib><creatorcontrib>Sazonova, Margarita A.</creatorcontrib><creatorcontrib>Orekhov, Alexander N.</creatorcontrib><title>Cybrid Models of Pathological Cell Processes in Different Diseases</title><title>Oxidative medicine and cellular longevity</title><addtitle>Oxid Med Cell Longev</addtitle><description>Modelling of pathological processes in cells is one of the most sought-after technologies of the 21st century. Using models of such processes may help to study the pathogenetic mechanisms of various diseases. The aim of the present study was to analyse the literature, dedicated to obtaining and investigating cybrid models. Besides, the possibility of modeling pathological processes in cells and treatment of different diseases using the models was evaluated. Methods of obtaining Rho0 cell cultures showed that, during their creation, mainly a standard technique, based on the use of mtDNA replication inhibitors (ethidium bromide), was applied. Cybrid lines were usually obtained by PEG fusion. Most frequently, platelets acted as donors of mitochondria. According to the analysis of the literature data, cybrid cell cultures can be modeled to study the dysfunction of the mitochondrial genome and molecular cellular pathological processes. Such models can be very promising for the development of therapeutic approaches to the treatment of various human diseases.</description><subject>Cell culture</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>DNA</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Mitochondria</subject><subject>Mitochondrial DNA</subject><subject>Mutation</subject><subject>Review</subject><issn>1942-0900</issn><issn>1942-0994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkUtPGzEURi0E4lV2rKuR2FSCgF_jsTdIEJ4SVVm0a8tjXydGkzHYSRH_HoeEAF115Sv76Ph--hDaJ_iYkLo-oZjIEy54QwlfQ9tEcTrASvH11YzxFtrJ-QFjwSgnm2iLKiWZrMU2Oh--tCm46md00OUq-ureTMexi6NgTVcNoeuq-xQt5Ay5Cn11EbyHBP20TBlMuf2GNrzpMuwtz1305-ry9_BmcPfr-nZ4djewXKnpwHlnHOfKCIZBMGCNsg2VgrctdYKAhxZLAURgZoBSKWnNKAjLHa1F4yXbRacL7-OsnYCzZYdkOv2YwsSkFx1N0F9f-jDWo_hXC0yKgRfBj6UgxacZ5KmehGxLQtNDnGVNsWgIIw1XBT34B32Is9SXeIWqpVSSC_ZBjUwHOvQ-ln_tXKrPBKYNaeo319GCsinmnMCvViZYzyvU8wr1ssKCf_8ccwW_d1aAwwUwDr0zz-E_dVAY8OaDpoRwUbNXnlerKw</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Sobenin, Igor A.</creator><creator>Postnov, Anton Y.</creator><creator>Melnichenko, Alexandra A.</creator><creator>Galitsyna, Elena V.</creator><creator>Ryzhkova, Anastasia I.</creator><creator>Sinyov, Vasily V.</creator><creator>Sazonova, Margarita A.</creator><creator>Orekhov, Alexander N.</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8610-4593</orcidid><orcidid>https://orcid.org/0000-0002-8838-7750</orcidid><orcidid>https://orcid.org/0000-0002-6495-1628</orcidid><orcidid>https://orcid.org/0000-0002-2501-7269</orcidid><orcidid>https://orcid.org/0000-0001-5105-5763</orcidid><orcidid>https://orcid.org/0000-0003-0978-6444</orcidid></search><sort><creationdate>20180101</creationdate><title>Cybrid Models of Pathological Cell Processes in Different Diseases</title><author>Sobenin, Igor A. ; Postnov, Anton Y. ; Melnichenko, Alexandra A. ; Galitsyna, Elena V. ; Ryzhkova, Anastasia I. ; Sinyov, Vasily V. ; Sazonova, Margarita A. ; Orekhov, Alexander N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-dfdad449a630e63e379c72864bb2d61efeb086e1603ae22882532e6c4d2567f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cell culture</topic><topic>Deoxyribonucleic acid</topic><topic>Disease</topic><topic>DNA</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Mitochondria</topic><topic>Mitochondrial DNA</topic><topic>Mutation</topic><topic>Review</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sobenin, Igor A.</creatorcontrib><creatorcontrib>Postnov, Anton Y.</creatorcontrib><creatorcontrib>Melnichenko, Alexandra A.</creatorcontrib><creatorcontrib>Galitsyna, Elena V.</creatorcontrib><creatorcontrib>Ryzhkova, Anastasia I.</creatorcontrib><creatorcontrib>Sinyov, Vasily V.</creatorcontrib><creatorcontrib>Sazonova, Margarita A.</creatorcontrib><creatorcontrib>Orekhov, Alexander N.</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oxidative medicine and cellular longevity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sobenin, Igor A.</au><au>Postnov, Anton Y.</au><au>Melnichenko, Alexandra A.</au><au>Galitsyna, Elena V.</au><au>Ryzhkova, Anastasia I.</au><au>Sinyov, Vasily V.</au><au>Sazonova, Margarita A.</au><au>Orekhov, Alexander N.</au><au>Ramana, Kota V.</au><au>Kota V Ramana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cybrid Models of Pathological Cell Processes in Different Diseases</atitle><jtitle>Oxidative medicine and cellular longevity</jtitle><addtitle>Oxid Med Cell Longev</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>2018</volume><issue>2018</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>1942-0900</issn><eissn>1942-0994</eissn><abstract>Modelling of pathological processes in cells is one of the most sought-after technologies of the 21st century. Using models of such processes may help to study the pathogenetic mechanisms of various diseases. The aim of the present study was to analyse the literature, dedicated to obtaining and investigating cybrid models. Besides, the possibility of modeling pathological processes in cells and treatment of different diseases using the models was evaluated. Methods of obtaining Rho0 cell cultures showed that, during their creation, mainly a standard technique, based on the use of mtDNA replication inhibitors (ethidium bromide), was applied. Cybrid lines were usually obtained by PEG fusion. Most frequently, platelets acted as donors of mitochondria. According to the analysis of the literature data, cybrid cell cultures can be modeled to study the dysfunction of the mitochondrial genome and molecular cellular pathological processes. Such models can be very promising for the development of therapeutic approaches to the treatment of various human diseases.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>29983856</pmid><doi>10.1155/2018/4647214</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-8610-4593</orcidid><orcidid>https://orcid.org/0000-0002-8838-7750</orcidid><orcidid>https://orcid.org/0000-0002-6495-1628</orcidid><orcidid>https://orcid.org/0000-0002-2501-7269</orcidid><orcidid>https://orcid.org/0000-0001-5105-5763</orcidid><orcidid>https://orcid.org/0000-0003-0978-6444</orcidid><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central; Alma/SFX Local Collection |
| subjects | Cell culture Deoxyribonucleic acid Disease DNA Genes Genomes Genomics Medical research Medicine, Experimental Mitochondria Mitochondrial DNA Mutation Review |
| title | Cybrid Models of Pathological Cell Processes in Different Diseases |
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