Characterization of a novel Gram‐stain‐positive anaerobic coccus isolated from the female genital tract: Genome sequence and description of Murdochiella vaginalis sp. nov

Strain Marseille‐P2341T, a nonmotile, nonspore‐forming, Gram‐stain‐positive anaerobic coccus, was isolated in the vaginal specimen of a patient with bacterial vaginosis using culturomics. Its growth occurred at temperatures ranging from 25 to 42°C, with pH between 6.5 and 8.5, and at NaCl concentrations lower than 5%. The major fatty acids were C18:1n9 (27.7%) and C16:0 (24.4%). Its genome is 1,671,491 bp long with 49.48 mol% of G+C content. It is composed of 1,501 genes: 1,446 were protein‐coding genes and 55 were RNAs. Strain Marseille‐P2341T shared 97.3% of 16S rRNA gene sequence similarity with Murdochiella asaccharolytica, the phylogenetically closest species. These results enabled the classification of strain Marseille‐P2341T as a new species of the genus Murdochiella for which we proposed the name Murdochiella vaginalis sp. nov. The type strain is strain Marseille‐P2341T (=DSM 102237, =CSUR P2341). Murdochiella vaginalis is a new bacterial species isolated in a vaginal sample from a patient with bacterial vaginosis. Murdochiella vaginalis is a Gram‐positive coccus‐shaped, nonmotile, and strictly anaerobic bacterium. The genome of M. vaginalis which 1,671,491 bp long contains 49.48 mol% G+C, 1,446 protein‐coding genes and 55 RNAs.