Occupational Exposure to Pesticides in Tobacco Fields: The Integrated Evaluation of Nutritional Intake and Susceptibility on Genomic and Epigenetic Instability
Pesticides used at tobacco fields are associated with genomic instability, which is proposed to be sensitive to nutritional intake and may also induce epigenetic changes. We evaluated the effect of dietary intake and genetic susceptibility polymorphisms in MTHFR (rs1801133) and TERT (rs2736100) gene...
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| Veröffentlicht in: | Oxidative medicine and cellular longevity 2018, Vol.2018 (2018), p.1-13 |
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| creator | Pedron, Tatiana Cappetta, Mónica Mártinez-López, Wilner Simon, Daniel Silva, Juliana da Cerchiaro, Giselle Nunes, Emilene Arusievicz Marroni, Norma Anair Possa da Silva, Fabiane Nitzke de Souza, Melissa Rosa Fenech, Michael Dhillon, V. S. Kahl, Vivian F. Silva Batista, Bruno Lemos |
| description | Pesticides used at tobacco fields are associated with genomic instability, which is proposed to be sensitive to nutritional intake and may also induce epigenetic changes. We evaluated the effect of dietary intake and genetic susceptibility polymorphisms in MTHFR (rs1801133) and TERT (rs2736100) genes on genomic and epigenetic instability in tobacco farmers. Farmers, when compared to a nonexposed group, showed increased levels of different parameters of DNA damage (micronuclei, nucleoplasmic bridges, and nuclear buds), evaluated by cytokinesis-block micronucleus cytome assay. Telomere length (TL) measured by quantitative PCR was shorter in exposed individuals. Global DNA methylation was significantly decreased in tobacco farmers. The exposed group had lower dietary intake of fiber, but an increase in cholesterol; vitamins such as B6, B12, and C; β-carotene; and α-retinol. Several trace and ultratrace elements were found higher in farmers than in nonfarmers. The MTHFR CT/TT genotype influenced nucleoplasmic bridges, nuclear buds, and TL in the exposed group, whereas TERT GT/TT only affected micronucleus frequency. We observed a positive correlation of TL and lipids and an inverse correlation of TL and fibers. The present data suggest an important role of dietary intake and subjects’ genetic susceptibility to xenobiotics-induced damages and epigenetic alterations in tobacco farmers occupationally exposed to mixtures of pesticides. |
| doi_str_mv | 10.1155/2018/7017423 |
| format | Article |
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S. ; Kahl, Vivian F. Silva ; Batista, Bruno Lemos</creator><contributor>Rachidi, Walid</contributor><creatorcontrib>Pedron, Tatiana ; Cappetta, Mónica ; Mártinez-López, Wilner ; Simon, Daniel ; Silva, Juliana da ; Cerchiaro, Giselle ; Nunes, Emilene Arusievicz ; Marroni, Norma Anair Possa ; da Silva, Fabiane Nitzke ; de Souza, Melissa Rosa ; Fenech, Michael ; Dhillon, V. S. ; Kahl, Vivian F. Silva ; Batista, Bruno Lemos ; Rachidi, Walid</creatorcontrib><description>Pesticides used at tobacco fields are associated with genomic instability, which is proposed to be sensitive to nutritional intake and may also induce epigenetic changes. We evaluated the effect of dietary intake and genetic susceptibility polymorphisms in MTHFR (rs1801133) and TERT (rs2736100) genes on genomic and epigenetic instability in tobacco farmers. Farmers, when compared to a nonexposed group, showed increased levels of different parameters of DNA damage (micronuclei, nucleoplasmic bridges, and nuclear buds), evaluated by cytokinesis-block micronucleus cytome assay. Telomere length (TL) measured by quantitative PCR was shorter in exposed individuals. Global DNA methylation was significantly decreased in tobacco farmers. The exposed group had lower dietary intake of fiber, but an increase in cholesterol; vitamins such as B6, B12, and C; β-carotene; and α-retinol. Several trace and ultratrace elements were found higher in farmers than in nonfarmers. The MTHFR CT/TT genotype influenced nucleoplasmic bridges, nuclear buds, and TL in the exposed group, whereas TERT GT/TT only affected micronucleus frequency. We observed a positive correlation of TL and lipids and an inverse correlation of TL and fibers. The present data suggest an important role of dietary intake and subjects’ genetic susceptibility to xenobiotics-induced damages and epigenetic alterations in tobacco farmers occupationally exposed to mixtures of pesticides.</description><identifier>ISSN: 1942-0900</identifier><identifier>EISSN: 1942-0994</identifier><identifier>DOI: 10.1155/2018/7017423</identifier><identifier>PMID: 29967663</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adult ; Beta carotene ; Brazil ; Diet ; Disease susceptibility ; DNA damage ; DNA Damage - drug effects ; DNA Damage - genetics ; DNA methylation ; Epigenetic inheritance ; Epigenetics ; Farmers ; Female ; Genes ; Genetic aspects ; Genetic polymorphisms ; Genetic Predisposition to Disease - genetics ; Genomic Instability - drug effects ; Genomic Instability - genetics ; Genotype ; Humans ; Male ; Methylation ; Methylenetetrahydrofolate Reductase (NADPH2) - genetics ; Middle Aged ; Mutation ; Nicotiana ; Occupational Exposure - adverse effects ; Pesticides ; Pesticides - adverse effects ; Polymorphism, Single Nucleotide ; Retinoids ; Studies ; Telomerase - genetics ; Telomere Shortening - drug effects ; Telomeres</subject><ispartof>Oxidative medicine and cellular longevity, 2018, Vol.2018 (2018), p.1-13</ispartof><rights>Copyright © 2018 Vivian F. Silva Kahl et al.</rights><rights>COPYRIGHT 2018 John Wiley & Sons, Inc.</rights><rights>Copyright © 2018 Vivian F. Silva Kahl et al.; This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2018 Vivian F. Silva Kahl et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4143-7fd55eddf4881e92865d9998c9b9aa7424ebdbf2e2c31a252af37be4f9a4a2773</citedby><cites>FETCH-LOGICAL-c4143-7fd55eddf4881e92865d9998c9b9aa7424ebdbf2e2c31a252af37be4f9a4a2773</cites><orcidid>0000-0002-7615-8694 ; 0000-0002-8466-0991 ; 0000-0003-2015-0156 ; 0000-0001-9908-9313 ; 0000-0003-1094-6302 ; 0000-0003-1122-8468 ; 0000-0003-3935-3324 ; 0000-0002-1089-6766</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009020/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009020/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29967663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Rachidi, Walid</contributor><creatorcontrib>Pedron, Tatiana</creatorcontrib><creatorcontrib>Cappetta, Mónica</creatorcontrib><creatorcontrib>Mártinez-López, Wilner</creatorcontrib><creatorcontrib>Simon, Daniel</creatorcontrib><creatorcontrib>Silva, Juliana da</creatorcontrib><creatorcontrib>Cerchiaro, Giselle</creatorcontrib><creatorcontrib>Nunes, Emilene Arusievicz</creatorcontrib><creatorcontrib>Marroni, Norma Anair Possa</creatorcontrib><creatorcontrib>da Silva, Fabiane Nitzke</creatorcontrib><creatorcontrib>de Souza, Melissa Rosa</creatorcontrib><creatorcontrib>Fenech, Michael</creatorcontrib><creatorcontrib>Dhillon, V. S.</creatorcontrib><creatorcontrib>Kahl, Vivian F. Silva</creatorcontrib><creatorcontrib>Batista, Bruno Lemos</creatorcontrib><title>Occupational Exposure to Pesticides in Tobacco Fields: The Integrated Evaluation of Nutritional Intake and Susceptibility on Genomic and Epigenetic Instability</title><title>Oxidative medicine and cellular longevity</title><addtitle>Oxid Med Cell Longev</addtitle><description>Pesticides used at tobacco fields are associated with genomic instability, which is proposed to be sensitive to nutritional intake and may also induce epigenetic changes. We evaluated the effect of dietary intake and genetic susceptibility polymorphisms in MTHFR (rs1801133) and TERT (rs2736100) genes on genomic and epigenetic instability in tobacco farmers. Farmers, when compared to a nonexposed group, showed increased levels of different parameters of DNA damage (micronuclei, nucleoplasmic bridges, and nuclear buds), evaluated by cytokinesis-block micronucleus cytome assay. Telomere length (TL) measured by quantitative PCR was shorter in exposed individuals. Global DNA methylation was significantly decreased in tobacco farmers. The exposed group had lower dietary intake of fiber, but an increase in cholesterol; vitamins such as B6, B12, and C; β-carotene; and α-retinol. Several trace and ultratrace elements were found higher in farmers than in nonfarmers. The MTHFR CT/TT genotype influenced nucleoplasmic bridges, nuclear buds, and TL in the exposed group, whereas TERT GT/TT only affected micronucleus frequency. We observed a positive correlation of TL and lipids and an inverse correlation of TL and fibers. The present data suggest an important role of dietary intake and subjects’ genetic susceptibility to xenobiotics-induced damages and epigenetic alterations in tobacco farmers occupationally exposed to mixtures of pesticides.</description><subject>Adult</subject><subject>Beta carotene</subject><subject>Brazil</subject><subject>Diet</subject><subject>Disease susceptibility</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>DNA Damage - genetics</subject><subject>DNA methylation</subject><subject>Epigenetic inheritance</subject><subject>Epigenetics</subject><subject>Farmers</subject><subject>Female</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic polymorphisms</subject><subject>Genetic Predisposition to Disease - genetics</subject><subject>Genomic Instability - drug effects</subject><subject>Genomic Instability - genetics</subject><subject>Genotype</subject><subject>Humans</subject><subject>Male</subject><subject>Methylation</subject><subject>Methylenetetrahydrofolate Reductase (NADPH2) - genetics</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Nicotiana</subject><subject>Occupational Exposure - adverse effects</subject><subject>Pesticides</subject><subject>Pesticides - adverse effects</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Retinoids</subject><subject>Studies</subject><subject>Telomerase - genetics</subject><subject>Telomere Shortening - drug effects</subject><subject>Telomeres</subject><issn>1942-0900</issn><issn>1942-0994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkcFu1DAURSMEoqVlxxpZYglDbceJYxZIVTUtlSpaqdO19WI_z7hk4mAnhX4Nv4rbGaawY2VL7_j46d6ieMPoR8aq6ohT1hxJyqTg5bNinynBZ1Qp8Xx3p3SveJXSLaV1yQV7WexxpWpZ1-V-8evSmGmA0YceOjL_OYQ0RSRjIFeYRm-8xUR8TxahBWMCOfXY2fSJLFZIzvsRlxFGtGR-B930aCHBka_TGP1WmSH4hgR6S66nZHAYfes7P96TzJ5hH9bePE7ng19ij_nP_CaNsKEOixcOuoSvt-dBcXM6X5x8mV1cnp2fHF_MjGCinElnqwqtdaJpGCre1JVVSjVGtQogRyOwta3jyE3JgFccXClbFE6BAC5leVB83niHqV2jNdiPETo9RL-GeK8DeP3vpPcrvQx3uqY5YE6z4N1WEMP3KWenb8MUcwJJc1pJypWsmydqCR1q37uQZWbtk9HHNeWSSV6zTH3YUCaGlCK63R6M6ofS9UPpelt6xt_-vfsO_tNyBt5vgJXvLfzw_6nDzKCDJ5ozpkpa_gY1ncEI</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Pedron, Tatiana</creator><creator>Cappetta, Mónica</creator><creator>Mártinez-López, Wilner</creator><creator>Simon, Daniel</creator><creator>Silva, Juliana da</creator><creator>Cerchiaro, Giselle</creator><creator>Nunes, Emilene Arusievicz</creator><creator>Marroni, Norma Anair Possa</creator><creator>da Silva, Fabiane Nitzke</creator><creator>de Souza, Melissa Rosa</creator><creator>Fenech, Michael</creator><creator>Dhillon, V. 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S.</au><au>Kahl, Vivian F. Silva</au><au>Batista, Bruno Lemos</au><au>Rachidi, Walid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occupational Exposure to Pesticides in Tobacco Fields: The Integrated Evaluation of Nutritional Intake and Susceptibility on Genomic and Epigenetic Instability</atitle><jtitle>Oxidative medicine and cellular longevity</jtitle><addtitle>Oxid Med Cell Longev</addtitle><date>2018</date><risdate>2018</risdate><volume>2018</volume><issue>2018</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>1942-0900</issn><eissn>1942-0994</eissn><abstract>Pesticides used at tobacco fields are associated with genomic instability, which is proposed to be sensitive to nutritional intake and may also induce epigenetic changes. We evaluated the effect of dietary intake and genetic susceptibility polymorphisms in MTHFR (rs1801133) and TERT (rs2736100) genes on genomic and epigenetic instability in tobacco farmers. Farmers, when compared to a nonexposed group, showed increased levels of different parameters of DNA damage (micronuclei, nucleoplasmic bridges, and nuclear buds), evaluated by cytokinesis-block micronucleus cytome assay. Telomere length (TL) measured by quantitative PCR was shorter in exposed individuals. Global DNA methylation was significantly decreased in tobacco farmers. The exposed group had lower dietary intake of fiber, but an increase in cholesterol; vitamins such as B6, B12, and C; β-carotene; and α-retinol. Several trace and ultratrace elements were found higher in farmers than in nonfarmers. The MTHFR CT/TT genotype influenced nucleoplasmic bridges, nuclear buds, and TL in the exposed group, whereas TERT GT/TT only affected micronucleus frequency. We observed a positive correlation of TL and lipids and an inverse correlation of TL and fibers. The present data suggest an important role of dietary intake and subjects’ genetic susceptibility to xenobiotics-induced damages and epigenetic alterations in tobacco farmers occupationally exposed to mixtures of pesticides.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>29967663</pmid><doi>10.1155/2018/7017423</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-7615-8694</orcidid><orcidid>https://orcid.org/0000-0002-8466-0991</orcidid><orcidid>https://orcid.org/0000-0003-2015-0156</orcidid><orcidid>https://orcid.org/0000-0001-9908-9313</orcidid><orcidid>https://orcid.org/0000-0003-1094-6302</orcidid><orcidid>https://orcid.org/0000-0003-1122-8468</orcidid><orcidid>https://orcid.org/0000-0003-3935-3324</orcidid><orcidid>https://orcid.org/0000-0002-1089-6766</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1942-0900 |
| ispartof | Oxidative medicine and cellular longevity, 2018, Vol.2018 (2018), p.1-13 |
| issn | 1942-0900 1942-0994 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6009020 |
| source | MEDLINE; Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Adult Beta carotene Brazil Diet Disease susceptibility DNA damage DNA Damage - drug effects DNA Damage - genetics DNA methylation Epigenetic inheritance Epigenetics Farmers Female Genes Genetic aspects Genetic polymorphisms Genetic Predisposition to Disease - genetics Genomic Instability - drug effects Genomic Instability - genetics Genotype Humans Male Methylation Methylenetetrahydrofolate Reductase (NADPH2) - genetics Middle Aged Mutation Nicotiana Occupational Exposure - adverse effects Pesticides Pesticides - adverse effects Polymorphism, Single Nucleotide Retinoids Studies Telomerase - genetics Telomere Shortening - drug effects Telomeres |
| title | Occupational Exposure to Pesticides in Tobacco Fields: The Integrated Evaluation of Nutritional Intake and Susceptibility on Genomic and Epigenetic Instability |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T23%3A26%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Occupational%20Exposure%20to%20Pesticides%20in%20Tobacco%20Fields:%20The%20Integrated%20Evaluation%20of%20Nutritional%20Intake%20and%20Susceptibility%20on%20Genomic%20and%20Epigenetic%20Instability&rft.jtitle=Oxidative%20medicine%20and%20cellular%20longevity&rft.au=Pedron,%20Tatiana&rft.date=2018&rft.volume=2018&rft.issue=2018&rft.spage=1&rft.epage=13&rft.pages=1-13&rft.issn=1942-0900&rft.eissn=1942-0994&rft_id=info:doi/10.1155/2018/7017423&rft_dat=%3Cgale_pubme%3EA602717261%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2057029768&rft_id=info:pmid/29967663&rft_galeid=A602717261&rfr_iscdi=true |