Mass Spectral Detection of Diethoxyphospho-Tyrosine Adducts on Proteins from HEK293 Cells Using Monoclonal Antibody depY for Enrichment

Chronic illness from exposure to organophosphorus toxicants is hypothesized to involve modification of unknown proteins. Tyrosine in proteins that have no active site serine readily reacts with organophosphorus toxicants. We developed a monoclonal antibody, depY, that specifically recognizes diethox...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chemical research in toxicology 2018-06, Vol.31 (6), p.520-530
Hauptverfasser:	Onder, Seda, Schopfer, Lawrence M, Tacal, Ozden, Blake, Thomas A, Johnson, Rudolph C, Lockridge, Oksana
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Blotting, Western Chlorpyrifos - analogs & derivatives Chlorpyrifos - chemistry Chromatography, Liquid Enzyme-Linked Immunosorbent Assay HEK293 Cells Humans Mice Molecular Structure Peptides - analysis Peptides - chemistry Peptides - immunology Proteins - analysis Proteins - chemistry Proteins - immunology Proteolysis Tandem Mass Spectrometry Tyrosine - analogs & derivatives Tyrosine - chemistry Tyrosine - immunology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!