Real Time Imaging and Dynamics of Hippocampal Zn2+ under Epileptic Condition Using a Ratiometric Fluorescent Probe
Zinc, the essential trace element in human body exists either in the bound or free state, for both structural and functional roles. Insights on Zn 2+ distribution and its dynamics are essential in view of the fact that Zn 2+ dyshomeostasis is a risk factor for epileptic seizures, Alzheimer’s disease...
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| Veröffentlicht in: | Scientific reports 2018-06, Vol.8 (1), p.1-11, Article 9069 |
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| creator | Santhakumar, Hema Nair, Resmi. V. Philips, Divya Susan Shenoy, Sachin J. Thekkuveettil, Anoopkumar Ajayaghosh, Ayyappanpillai Jayasree, Ramapurath. S. |
| description | Zinc, the essential trace element in human body exists either in the bound or free state, for both structural and functional roles. Insights on Zn
2+
distribution and its dynamics are essential in view of the fact that Zn
2+
dyshomeostasis is a risk factor for epileptic seizures, Alzheimer’s disease, depression, etc. Herein, a bipyridine bridged bispyrrole (BP) probe is used for ratiometric imaging and quantification of Zn
2+
in hippocampal slices. The green fluorescence emission of BP shifts towards red in the presence of Zn
2+
. The probe is used to detect and quantify the exogenous and endogenous Zn
2+
in glioma cells and hippocampal slices. The dynamics of chelatable zinc ions during epileptic condition is studied in the hippocampal neurons,
in vitro
wherein the translocation of Zn
2+
from presynaptic to postsynaptic neuronal bodies is imaged and ratiometrically quantified. Raman mapping technique is used to confirm the dynamics of Zn
2+
under epileptic condition. Finally, the Zn
2+
distribution was imaged
in vivo
in epileptic rats and the total Zn
2+
in rat brain was quantified. The results favour the use of BP as an excellent Zn
2+
imaging probe in biological system to understand the zinc associated diseases and their management. |
| doi_str_mv | 10.1038/s41598-018-27029-5 |
| format | Article |
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2+
distribution and its dynamics are essential in view of the fact that Zn
2+
dyshomeostasis is a risk factor for epileptic seizures, Alzheimer’s disease, depression, etc. Herein, a bipyridine bridged bispyrrole (BP) probe is used for ratiometric imaging and quantification of Zn
2+
in hippocampal slices. The green fluorescence emission of BP shifts towards red in the presence of Zn
2+
. The probe is used to detect and quantify the exogenous and endogenous Zn
2+
in glioma cells and hippocampal slices. The dynamics of chelatable zinc ions during epileptic condition is studied in the hippocampal neurons,
in vitro
wherein the translocation of Zn
2+
from presynaptic to postsynaptic neuronal bodies is imaged and ratiometrically quantified. Raman mapping technique is used to confirm the dynamics of Zn
2+
under epileptic condition. Finally, the Zn
2+
distribution was imaged
in vivo
in epileptic rats and the total Zn
2+
in rat brain was quantified. The results favour the use of BP as an excellent Zn
2+
imaging probe in biological system to understand the zinc associated diseases and their management.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-27029-5</identifier><identifier>PMID: 29899532</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14 ; 14/10 ; 14/63 ; 140/133 ; 59 ; 631/1647/245/2226 ; 631/378/1689/178 ; Brain slice preparation ; Epilepsy ; Fluorescent indicators ; Glioma cells ; Hippocampus ; Humanities and Social Sciences ; Mental depression ; multidisciplinary ; Neuroimaging ; Risk factors ; Science ; Science (multidisciplinary) ; Seizures ; Structure-function relationships ; Trace elements ; Translocation ; Zinc</subject><ispartof>Scientific reports, 2018-06, Vol.8 (1), p.1-11, Article 9069</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-335211b8e3c3f7762f025847192efce5ea0b73a168e442a59331e0d2ae4b6b773</citedby><cites>FETCH-LOGICAL-c381t-335211b8e3c3f7762f025847192efce5ea0b73a168e442a59331e0d2ae4b6b773</cites><orcidid>0000-0001-8574-5391 ; 0000-0002-2139-2862 ; 0000-0001-6810-9879</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998144/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998144/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51555,53770,53772</link.rule.ids></links><search><creatorcontrib>Santhakumar, Hema</creatorcontrib><creatorcontrib>Nair, Resmi. V.</creatorcontrib><creatorcontrib>Philips, Divya Susan</creatorcontrib><creatorcontrib>Shenoy, Sachin J.</creatorcontrib><creatorcontrib>Thekkuveettil, Anoopkumar</creatorcontrib><creatorcontrib>Ajayaghosh, Ayyappanpillai</creatorcontrib><creatorcontrib>Jayasree, Ramapurath. S.</creatorcontrib><title>Real Time Imaging and Dynamics of Hippocampal Zn2+ under Epileptic Condition Using a Ratiometric Fluorescent Probe</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><description>Zinc, the essential trace element in human body exists either in the bound or free state, for both structural and functional roles. Insights on Zn
2+
distribution and its dynamics are essential in view of the fact that Zn
2+
dyshomeostasis is a risk factor for epileptic seizures, Alzheimer’s disease, depression, etc. Herein, a bipyridine bridged bispyrrole (BP) probe is used for ratiometric imaging and quantification of Zn
2+
in hippocampal slices. The green fluorescence emission of BP shifts towards red in the presence of Zn
2+
. The probe is used to detect and quantify the exogenous and endogenous Zn
2+
in glioma cells and hippocampal slices. The dynamics of chelatable zinc ions during epileptic condition is studied in the hippocampal neurons,
in vitro
wherein the translocation of Zn
2+
from presynaptic to postsynaptic neuronal bodies is imaged and ratiometrically quantified. Raman mapping technique is used to confirm the dynamics of Zn
2+
under epileptic condition. Finally, the Zn
2+
distribution was imaged
in vivo
in epileptic rats and the total Zn
2+
in rat brain was quantified. The results favour the use of BP as an excellent Zn
2+
imaging probe in biological system to understand the zinc associated diseases and their management.</description><subject>14</subject><subject>14/10</subject><subject>14/63</subject><subject>140/133</subject><subject>59</subject><subject>631/1647/245/2226</subject><subject>631/378/1689/178</subject><subject>Brain slice preparation</subject><subject>Epilepsy</subject><subject>Fluorescent indicators</subject><subject>Glioma cells</subject><subject>Hippocampus</subject><subject>Humanities and Social Sciences</subject><subject>Mental depression</subject><subject>multidisciplinary</subject><subject>Neuroimaging</subject><subject>Risk factors</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Seizures</subject><subject>Structure-function relationships</subject><subject>Trace elements</subject><subject>Translocation</subject><subject>Zinc</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcFrFTEQxoMottT-A54CvQiyNZkku8lFkGdrCwWltBcvIbs7-5qym6zJrtD_3ry-YtWDc8mE-X0fM3yEvOXslDOhP2TJldEV47qChoGp1AtyCEyqCgTAyz_6A3Kc8z0rpcBIbl6TAzDaGCXgkKRrdCO98RPSy8ltfdhSF3r6-SG4yXeZxoFe-HmOnZvmAn4P8J6uocdEz2Y_4rz4jm5i6P3iY6C3-dGAXrvynXBJZXo-rjFh7jAs9FuKLb4hrwY3Zjx-eo_I7fnZzeaiuvr65XLz6arqhOZLJYQCzluNohND09QwMFBaNtwADh0qdKxthOO1RinBKSMER9aDQ9nWbdOII_Jx7zuv7YT9boHkRjsnP7n0YKPz9u9J8Hd2G39aZYzmUhaDd08GKf5YMS928uWOcXQB45otMKVqXhuxQ0_-Qe_jmkI5b0dJrQwXolCwp7oUc044_F6GM7tL1e5TtSVV-5iqVUUk9qJc4LDF9Gz9H9UvFLKjWw</recordid><startdate>20180613</startdate><enddate>20180613</enddate><creator>Santhakumar, Hema</creator><creator>Nair, Resmi. 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V.</au><au>Philips, Divya Susan</au><au>Shenoy, Sachin J.</au><au>Thekkuveettil, Anoopkumar</au><au>Ajayaghosh, Ayyappanpillai</au><au>Jayasree, Ramapurath. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real Time Imaging and Dynamics of Hippocampal Zn2+ under Epileptic Condition Using a Ratiometric Fluorescent Probe</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><date>2018-06-13</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><artnum>9069</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Zinc, the essential trace element in human body exists either in the bound or free state, for both structural and functional roles. Insights on Zn
2+
distribution and its dynamics are essential in view of the fact that Zn
2+
dyshomeostasis is a risk factor for epileptic seizures, Alzheimer’s disease, depression, etc. Herein, a bipyridine bridged bispyrrole (BP) probe is used for ratiometric imaging and quantification of Zn
2+
in hippocampal slices. The green fluorescence emission of BP shifts towards red in the presence of Zn
2+
. The probe is used to detect and quantify the exogenous and endogenous Zn
2+
in glioma cells and hippocampal slices. The dynamics of chelatable zinc ions during epileptic condition is studied in the hippocampal neurons,
in vitro
wherein the translocation of Zn
2+
from presynaptic to postsynaptic neuronal bodies is imaged and ratiometrically quantified. Raman mapping technique is used to confirm the dynamics of Zn
2+
under epileptic condition. Finally, the Zn
2+
distribution was imaged
in vivo
in epileptic rats and the total Zn
2+
in rat brain was quantified. The results favour the use of BP as an excellent Zn
2+
imaging probe in biological system to understand the zinc associated diseases and their management.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29899532</pmid><doi>10.1038/s41598-018-27029-5</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8574-5391</orcidid><orcidid>https://orcid.org/0000-0002-2139-2862</orcidid><orcidid>https://orcid.org/0000-0001-6810-9879</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 14 14/10 14/63 140/133 59 631/1647/245/2226 631/378/1689/178 Brain slice preparation Epilepsy Fluorescent indicators Glioma cells Hippocampus Humanities and Social Sciences Mental depression multidisciplinary Neuroimaging Risk factors Science Science (multidisciplinary) Seizures Structure-function relationships Trace elements Translocation Zinc |
| title | Real Time Imaging and Dynamics of Hippocampal Zn2+ under Epileptic Condition Using a Ratiometric Fluorescent Probe |
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