Skin Microbiota in Obese Women at Risk for Surgical Site Infection After Cesarean Delivery
The obesity pandemic in the obstetrical population plus increased frequency of Cesarean delivery (CD) has increased vulnerability to surgical site infection (SSI). Here we characterized the microbiome at the site of skin incision before and after CD. Skin and relevant surgical sites were sampled bef...
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| Veröffentlicht in: | Scientific reports 2018-06, Vol.8 (1), p.8756-8, Article 8756 |
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| creator | Rood, Kara M. Buhimschi, Irina A. Jurcisek, Joseph A. Summerfield, Taryn L. Zhao, Guomao Ackerman, William E. Wang, Weiwei Rumpf, R. Wolfgang Thung, Stephen F. Bakaletz, Lauren O. Buhimschi, Catalin S. |
| description | The obesity pandemic in the obstetrical population plus increased frequency of Cesarean delivery (CD) has increased vulnerability to surgical site infection (SSI). Here we characterized the microbiome at the site of skin incision before and after CD. Skin and relevant surgical sites were sampled before and after surgical antisepsis from obese (n = 31) and non-obese (n = 27) pregnant women. We quantified bacterial biomass by qPCR, microbial community composition by 16sRNA sequencing, assigned operational taxonomic units, and stained skin biopsies from incision for bacteria and biofilms. In obese women, incision site harbors significantly higher bacterial biomass of lower diversity. Phylum
Firmicutes
predominated over
Actinobacteria
, with phylotypes
Clostridales
and
Bacteroidales
over commensal
Staphylococcus
and
Propionbacterium
spp. Skin dysbiosis increased post-surgical prep and at end of surgery. Biofilms were identified post-prep in the majority (73%) of skin biopsies. At end of surgery, incision had significant gains in bacterial DNA and diversity, and obese women shared more genera with vagina and surgeon’s glove in CD. Our findings suggest microbiota at incision differs between obese and non-obese pregnant women, and changes throughout CD. An interaction between vaginal and cutaneous dysbiosis at the incision site may explain the a priori increased risk for SSI among obese pregnant women. |
| doi_str_mv | 10.1038/s41598-018-27134-5 |
| format | Article |
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Firmicutes
predominated over
Actinobacteria
, with phylotypes
Clostridales
and
Bacteroidales
over commensal
Staphylococcus
and
Propionbacterium
spp. Skin dysbiosis increased post-surgical prep and at end of surgery. Biofilms were identified post-prep in the majority (73%) of skin biopsies. At end of surgery, incision had significant gains in bacterial DNA and diversity, and obese women shared more genera with vagina and surgeon’s glove in CD. Our findings suggest microbiota at incision differs between obese and non-obese pregnant women, and changes throughout CD. An interaction between vaginal and cutaneous dysbiosis at the incision site may explain the a priori increased risk for SSI among obese pregnant women.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-27134-5</identifier><identifier>PMID: 29884793</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/22 ; 38/23 ; 38/77 ; 45/41 ; 692/308/575 ; 692/420/254 ; 692/499 ; Bacteria ; Biofilms ; Biomass ; Biopsy ; Cesarean section ; Community composition ; Deoxyribonucleic acid ; DNA ; Dysbacteriosis ; Genera ; Humanities and Social Sciences ; Microbiomes ; Microbiota ; multidisciplinary ; Obesity ; Pandemics ; Pregnancy ; Science ; Science (multidisciplinary) ; Skin ; Surgery ; Surgical site infections ; Vagina</subject><ispartof>Scientific reports, 2018-06, Vol.8 (1), p.8756-8, Article 8756</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-c520b85f3ee7d3d2ba5d9e9cf8462be6d9dfb3d4fd83bde9e4dd5c4c28d029393</citedby><cites>FETCH-LOGICAL-c513t-c520b85f3ee7d3d2ba5d9e9cf8462be6d9dfb3d4fd83bde9e4dd5c4c28d029393</cites><orcidid>0000-0002-8504-6715</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993816/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993816/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29884793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rood, Kara M.</creatorcontrib><creatorcontrib>Buhimschi, Irina A.</creatorcontrib><creatorcontrib>Jurcisek, Joseph A.</creatorcontrib><creatorcontrib>Summerfield, Taryn L.</creatorcontrib><creatorcontrib>Zhao, Guomao</creatorcontrib><creatorcontrib>Ackerman, William E.</creatorcontrib><creatorcontrib>Wang, Weiwei</creatorcontrib><creatorcontrib>Rumpf, R. Wolfgang</creatorcontrib><creatorcontrib>Thung, Stephen F.</creatorcontrib><creatorcontrib>Bakaletz, Lauren O.</creatorcontrib><creatorcontrib>Buhimschi, Catalin S.</creatorcontrib><title>Skin Microbiota in Obese Women at Risk for Surgical Site Infection After Cesarean Delivery</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The obesity pandemic in the obstetrical population plus increased frequency of Cesarean delivery (CD) has increased vulnerability to surgical site infection (SSI). Here we characterized the microbiome at the site of skin incision before and after CD. Skin and relevant surgical sites were sampled before and after surgical antisepsis from obese (n = 31) and non-obese (n = 27) pregnant women. We quantified bacterial biomass by qPCR, microbial community composition by 16sRNA sequencing, assigned operational taxonomic units, and stained skin biopsies from incision for bacteria and biofilms. In obese women, incision site harbors significantly higher bacterial biomass of lower diversity. Phylum
Firmicutes
predominated over
Actinobacteria
, with phylotypes
Clostridales
and
Bacteroidales
over commensal
Staphylococcus
and
Propionbacterium
spp. Skin dysbiosis increased post-surgical prep and at end of surgery. Biofilms were identified post-prep in the majority (73%) of skin biopsies. At end of surgery, incision had significant gains in bacterial DNA and diversity, and obese women shared more genera with vagina and surgeon’s glove in CD. Our findings suggest microbiota at incision differs between obese and non-obese pregnant women, and changes throughout CD. An interaction between vaginal and cutaneous dysbiosis at the incision site may explain the a priori increased risk for SSI among obese pregnant women.</description><subject>38/22</subject><subject>38/23</subject><subject>38/77</subject><subject>45/41</subject><subject>692/308/575</subject><subject>692/420/254</subject><subject>692/499</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>Biomass</subject><subject>Biopsy</subject><subject>Cesarean section</subject><subject>Community composition</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Dysbacteriosis</subject><subject>Genera</subject><subject>Humanities and Social Sciences</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>multidisciplinary</subject><subject>Obesity</subject><subject>Pandemics</subject><subject>Pregnancy</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Skin</subject><subject>Surgery</subject><subject>Surgical site infections</subject><subject>Vagina</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1PVDEUhhsjEYL8ARemiRs3F_s5025MyKhIgiFxNCRsmt72dCzcaaG9l4R_b4dBBBd20Y-c57yn57wIvaHkkBKuPlRBpVYdoapjc8pFJ1-gPUaE7Bhn7OWT-y46qPWStCWZFlS_QrtMKyXmmu-hi-VVTPhbdCX3MY8Wt9dZDxXweV5DwnbE32O9wiEXvJzKKjo74GUcAZ-kAG6MOeGjMELBC6i2gE34EwzxFsrda7QT7FDh4OHcRz-_fP6x-Nqdnh2fLI5OOycpH9vOSK9k4ABzzz3rrfQatAtKzFgPM6996LkXwSvee9AgvJdOOKY8YZprvo8-bnWvp34N3kEaix3MdYlrW-5MttE8j6T4y6zyrZFac0VnTeD9g0DJNxPU0axjdTAMNkGeqmFtcIrwudjUevcPepmnklp795RUmumNINtSbaq1FgiPn6HEbNwzW_dMc8_cu2dkS3r7tI3HlD9eNYBvgdpCaQXlb-3_yP4GCWmmXA</recordid><startdate>20180608</startdate><enddate>20180608</enddate><creator>Rood, Kara M.</creator><creator>Buhimschi, Irina A.</creator><creator>Jurcisek, Joseph A.</creator><creator>Summerfield, Taryn L.</creator><creator>Zhao, Guomao</creator><creator>Ackerman, William E.</creator><creator>Wang, Weiwei</creator><creator>Rumpf, R. 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Wolfgang</au><au>Thung, Stephen F.</au><au>Bakaletz, Lauren O.</au><au>Buhimschi, Catalin S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Skin Microbiota in Obese Women at Risk for Surgical Site Infection After Cesarean Delivery</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-06-08</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>8756</spage><epage>8</epage><pages>8756-8</pages><artnum>8756</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The obesity pandemic in the obstetrical population plus increased frequency of Cesarean delivery (CD) has increased vulnerability to surgical site infection (SSI). Here we characterized the microbiome at the site of skin incision before and after CD. Skin and relevant surgical sites were sampled before and after surgical antisepsis from obese (n = 31) and non-obese (n = 27) pregnant women. We quantified bacterial biomass by qPCR, microbial community composition by 16sRNA sequencing, assigned operational taxonomic units, and stained skin biopsies from incision for bacteria and biofilms. In obese women, incision site harbors significantly higher bacterial biomass of lower diversity. Phylum
Firmicutes
predominated over
Actinobacteria
, with phylotypes
Clostridales
and
Bacteroidales
over commensal
Staphylococcus
and
Propionbacterium
spp. Skin dysbiosis increased post-surgical prep and at end of surgery. Biofilms were identified post-prep in the majority (73%) of skin biopsies. At end of surgery, incision had significant gains in bacterial DNA and diversity, and obese women shared more genera with vagina and surgeon’s glove in CD. Our findings suggest microbiota at incision differs between obese and non-obese pregnant women, and changes throughout CD. An interaction between vaginal and cutaneous dysbiosis at the incision site may explain the a priori increased risk for SSI among obese pregnant women.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29884793</pmid><doi>10.1038/s41598-018-27134-5</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-8504-6715</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 38/22 38/23 38/77 45/41 692/308/575 692/420/254 692/499 Bacteria Biofilms Biomass Biopsy Cesarean section Community composition Deoxyribonucleic acid DNA Dysbacteriosis Genera Humanities and Social Sciences Microbiomes Microbiota multidisciplinary Obesity Pandemics Pregnancy Science Science (multidisciplinary) Skin Surgery Surgical site infections Vagina |
| title | Skin Microbiota in Obese Women at Risk for Surgical Site Infection After Cesarean Delivery |
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