Induction of apomixis by dimethyl sulfoxide (DMSO) and genetic identification of apomictic plants in cassava
Apomixis, or asexual seed formation, is of great value for plant breeding and seed production, and is desirable in modern agriculture, but natural apomixis occurs in cassava at very low frequency. In present study, apomixis was induced by the treatments of female flower buds with 1%, 1.5% and 2% (v/...
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| Veröffentlicht in: | Breeding Science 2018, Vol.68(2), pp.227-232 |
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| creator | Chen, Xia Lai, Hang-Gui Sun, Qi Liu, Jin-Ping Chen, Song-Bi Zhu, Wen-Li |
| description | Apomixis, or asexual seed formation, is of great value for plant breeding and seed production, and is desirable in modern agriculture, but natural apomixis occurs in cassava at very low frequency. In present study, apomixis was induced by the treatments of female flower buds with 1%, 1.5% and 2% (v/v) dimethyl sulfoxide (DMSO) and the results showed that 1.5% DMSO treatment was most effective for the induction of apomictic seed formation in cassava cultivar SC5 with the highest percentages of fruit set and true apomictic seeds. The germinated seedlings resembled their parents and displayed no morphological characteristics of cassava polyploid. Flow cytometry and chromosome counting showed that these plants were uniform diploids. Analysis of 34 DMSO-induced cassava progenies by the expressed sequence tag-simple sequence repeat (EST-SSR) and sequence-related amplified polymorphism (SRAP) markers showed that three true apomictic seeds were obtained from the group of SC5 treated with 1.5% DMSO. |
| doi_str_mv | 10.1270/jsbbs.17089 |
| format | Article |
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In present study, apomixis was induced by the treatments of female flower buds with 1%, 1.5% and 2% (v/v) dimethyl sulfoxide (DMSO) and the results showed that 1.5% DMSO treatment was most effective for the induction of apomictic seed formation in cassava cultivar SC5 with the highest percentages of fruit set and true apomictic seeds. The germinated seedlings resembled their parents and displayed no morphological characteristics of cassava polyploid. Flow cytometry and chromosome counting showed that these plants were uniform diploids. Analysis of 34 DMSO-induced cassava progenies by the expressed sequence tag-simple sequence repeat (EST-SSR) and sequence-related amplified polymorphism (SRAP) markers showed that three true apomictic seeds were obtained from the group of SC5 treated with 1.5% DMSO.</description><identifier>ISSN: 1344-7610</identifier><identifier>EISSN: 1347-3735</identifier><identifier>DOI: 10.1270/jsbbs.17089</identifier><identifier>PMID: 29875606</identifier><language>eng</language><publisher>Japan: Japanese Society of Breeding</publisher><subject>Apomixis ; Cassava ; Dimethyl sulfoxide ; Diploids ; DMSO ; EST-SSR markers ; Expressed sequence tags ; Flow cytometry ; Fruit set ; induced apomixis ; Manihot esculenta ; Parents ; Physical characteristics ; Plant breeding ; Polymorphism ; Polyploidy ; Research Paper ; Seedlings ; Seeds ; SRAP markers ; Sulfoxides</subject><ispartof>Breeding Science, 2018, Vol.68(2), pp.227-232</ispartof><rights>2018 by JAPANESE SOCIETY OF BREEDING</rights><rights>Copyright Japan Science and Technology Agency 2018</rights><rights>Copyright © 2018 by JAPANESE SOCIETY OF BREEDING 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c617t-6469579ecf0767a0438f2551622e04700b250f28c44dc027bed2c5d8ffa8bab83</citedby><cites>FETCH-LOGICAL-c617t-6469579ecf0767a0438f2551622e04700b250f28c44dc027bed2c5d8ffa8bab83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982182/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982182/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,1883,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29875606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xia</creatorcontrib><creatorcontrib>Lai, Hang-Gui</creatorcontrib><creatorcontrib>Sun, Qi</creatorcontrib><creatorcontrib>Liu, Jin-Ping</creatorcontrib><creatorcontrib>Chen, Song-Bi</creatorcontrib><creatorcontrib>Zhu, Wen-Li</creatorcontrib><title>Induction of apomixis by dimethyl sulfoxide (DMSO) and genetic identification of apomictic plants in cassava</title><title>Breeding Science</title><addtitle>Breeding Science</addtitle><description>Apomixis, or asexual seed formation, is of great value for plant breeding and seed production, and is desirable in modern agriculture, but natural apomixis occurs in cassava at very low frequency. In present study, apomixis was induced by the treatments of female flower buds with 1%, 1.5% and 2% (v/v) dimethyl sulfoxide (DMSO) and the results showed that 1.5% DMSO treatment was most effective for the induction of apomictic seed formation in cassava cultivar SC5 with the highest percentages of fruit set and true apomictic seeds. The germinated seedlings resembled their parents and displayed no morphological characteristics of cassava polyploid. Flow cytometry and chromosome counting showed that these plants were uniform diploids. Analysis of 34 DMSO-induced cassava progenies by the expressed sequence tag-simple sequence repeat (EST-SSR) and sequence-related amplified polymorphism (SRAP) markers showed that three true apomictic seeds were obtained from the group of SC5 treated with 1.5% DMSO.</description><subject>Apomixis</subject><subject>Cassava</subject><subject>Dimethyl sulfoxide</subject><subject>Diploids</subject><subject>DMSO</subject><subject>EST-SSR markers</subject><subject>Expressed sequence tags</subject><subject>Flow cytometry</subject><subject>Fruit set</subject><subject>induced apomixis</subject><subject>Manihot esculenta</subject><subject>Parents</subject><subject>Physical characteristics</subject><subject>Plant breeding</subject><subject>Polymorphism</subject><subject>Polyploidy</subject><subject>Research Paper</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>SRAP markers</subject><subject>Sulfoxides</subject><issn>1344-7610</issn><issn>1347-3735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkc1rFDEYxoMotlZP3iXgpSJT853MRSi11UKlB_UcMplkN8tMsiYzpfvfm_1wsV6S8D4_Hp43DwBvMbrARKJPq9J15QJLpNpn4BRTJhsqKX--e7NGCoxOwKtSVggRjhh_CU5IqyQXSJyC4Tb2s51CijB5aNZpDI-hwG4D-zC6abkZYJkHnx5D7-D5l-8_7j9AE3u4cNFNwcI6jlPwwZonHnarrQcTpwJDhNaUYh7Ma_DCm6G4N4f7DPy6uf559a25u_96e3V511iB5dQIJlouW2c9kkIaxKjyhHMsCHGISYS6uocnyjLWW0Rk53piea-8N6oznaJn4PPedz13o-ttjZjNoNc5jCZvdDJBP1ViWOpFetC8VQQrUg3ODwY5_Z5dmfQYinVDXciluWiCahqBMaMVff8fukpzjnW9SjFJWkwpr9THPWVzKiU7fwyDkd62qHct6l2LlX73b_4j-7e2ClzugVWZzMIdAZPrvw_uYCaUJttjZ3rU7NJk7SL9A7LtsZQ</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Chen, Xia</creator><creator>Lai, Hang-Gui</creator><creator>Sun, Qi</creator><creator>Liu, Jin-Ping</creator><creator>Chen, Song-Bi</creator><creator>Zhu, Wen-Li</creator><general>Japanese Society of Breeding</general><general>Japan Science and Technology Agency</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180301</creationdate><title>Induction of apomixis by dimethyl sulfoxide (DMSO) and genetic identification of apomictic plants in cassava</title><author>Chen, Xia ; Lai, Hang-Gui ; Sun, Qi ; Liu, Jin-Ping ; Chen, Song-Bi ; Zhu, Wen-Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c617t-6469579ecf0767a0438f2551622e04700b250f28c44dc027bed2c5d8ffa8bab83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apomixis</topic><topic>Cassava</topic><topic>Dimethyl sulfoxide</topic><topic>Diploids</topic><topic>DMSO</topic><topic>EST-SSR markers</topic><topic>Expressed sequence tags</topic><topic>Flow cytometry</topic><topic>Fruit set</topic><topic>induced apomixis</topic><topic>Manihot esculenta</topic><topic>Parents</topic><topic>Physical characteristics</topic><topic>Plant breeding</topic><topic>Polymorphism</topic><topic>Polyploidy</topic><topic>Research Paper</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>SRAP markers</topic><topic>Sulfoxides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xia</creatorcontrib><creatorcontrib>Lai, Hang-Gui</creatorcontrib><creatorcontrib>Sun, Qi</creatorcontrib><creatorcontrib>Liu, Jin-Ping</creatorcontrib><creatorcontrib>Chen, Song-Bi</creatorcontrib><creatorcontrib>Zhu, Wen-Li</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Breeding Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xia</au><au>Lai, Hang-Gui</au><au>Sun, Qi</au><au>Liu, Jin-Ping</au><au>Chen, Song-Bi</au><au>Zhu, Wen-Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of apomixis by dimethyl sulfoxide (DMSO) and genetic identification of apomictic plants in cassava</atitle><jtitle>Breeding Science</jtitle><addtitle>Breeding Science</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>68</volume><issue>2</issue><spage>227</spage><epage>232</epage><pages>227-232</pages><issn>1344-7610</issn><eissn>1347-3735</eissn><abstract>Apomixis, or asexual seed formation, is of great value for plant breeding and seed production, and is desirable in modern agriculture, but natural apomixis occurs in cassava at very low frequency. In present study, apomixis was induced by the treatments of female flower buds with 1%, 1.5% and 2% (v/v) dimethyl sulfoxide (DMSO) and the results showed that 1.5% DMSO treatment was most effective for the induction of apomictic seed formation in cassava cultivar SC5 with the highest percentages of fruit set and true apomictic seeds. The germinated seedlings resembled their parents and displayed no morphological characteristics of cassava polyploid. Flow cytometry and chromosome counting showed that these plants were uniform diploids. Analysis of 34 DMSO-induced cassava progenies by the expressed sequence tag-simple sequence repeat (EST-SSR) and sequence-related amplified polymorphism (SRAP) markers showed that three true apomictic seeds were obtained from the group of SC5 treated with 1.5% DMSO.</abstract><cop>Japan</cop><pub>Japanese Society of Breeding</pub><pmid>29875606</pmid><doi>10.1270/jsbbs.17089</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apomixis Cassava Dimethyl sulfoxide Diploids DMSO EST-SSR markers Expressed sequence tags Flow cytometry Fruit set induced apomixis Manihot esculenta Parents Physical characteristics Plant breeding Polymorphism Polyploidy Research Paper Seedlings Seeds SRAP markers Sulfoxides |
| title | Induction of apomixis by dimethyl sulfoxide (DMSO) and genetic identification of apomictic plants in cassava |
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