Humanization of fibroblast growth factor 1 single‐chain antibody and validation for its antitumorigenic efficacy in breast cancer and glioma cells
Single‐chain variable fragment (scFv) antibodies are the smallest immunoglobulins with high antigen‐binding affinity. We have previously reported that fibroblast growth factor 1 played pivotal roles in cancer development and generated a mouse scFv (mscFv1C9) could effectively prohibit cancer cell pr...
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| Veröffentlicht in: | Journal of cellular and molecular medicine 2018-06, Vol.22 (6), p.3259-3263 |
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| creator | He, Xiao‐Xiao Du, Shuang Gao, Shi‐Qian Chen, Jing‐Ying Cao, Ran‐Juan Xing, Zhen‐Kai Kazim, Alia Rizvi Syeda Yu, Hua‐Li Zheng, Qing‐Chuan Zhu, Xiao‐Juan |
| description | Single‐chain variable fragment (scFv) antibodies are the smallest immunoglobulins with high antigen‐binding affinity. We have previously reported that fibroblast growth factor 1 played pivotal roles in cancer development and generated a mouse scFv (mscFv1C9) could effectively prohibit cancer cell proliferation in vitro and in vivo. Here, we further humanized this scFv (hscFv1C9) using a structure‐guided complementarity determining region grafting strategy. The purified hscFv1C9 maintained similar antigen‐binding affinity and specificity as mscFv1C9, and it was capable of inhibiting growth of different tumours in vitro and in vivo. These data strongly suggested that hscFv1C9 has antitumour potentials. |
| doi_str_mv | 10.1111/jcmm.13547 |
| format | Article |
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We have previously reported that fibroblast growth factor 1 played pivotal roles in cancer development and generated a mouse scFv (mscFv1C9) could effectively prohibit cancer cell proliferation in vitro and in vivo. Here, we further humanized this scFv (hscFv1C9) using a structure‐guided complementarity determining region grafting strategy. The purified hscFv1C9 maintained similar antigen‐binding affinity and specificity as mscFv1C9, and it was capable of inhibiting growth of different tumours in vitro and in vivo. These data strongly suggested that hscFv1C9 has antitumour potentials.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/jcmm.13547</identifier><identifier>PMID: 29575613</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Affinity ; Amino Acid Sequence - genetics ; Amino acids ; Animals ; Antibodies ; Antibodies, Monoclonal, Humanized - immunology ; Antibodies, Monoclonal, Humanized - pharmacology ; antibody humanization ; Antigens ; Breast cancer ; Breast Neoplasms - drug therapy ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Cancer ; cancer treatment ; Cell cycle ; Cell division ; Cell growth ; Cell Line, Tumor ; Cell proliferation ; Cell Proliferation - drug effects ; Complementarity ; Enzyme-Linked Immunosorbent Assay ; Female ; Fibroblast growth factor 1 ; Fibroblast Growth Factor 1 - antagonists & inhibitors ; Fibroblast Growth Factor 1 - chemistry ; Fibroblast Growth Factor 1 - genetics ; Fibroblast Growth Factor 1 - immunology ; Fibroblasts ; Glioma ; Glioma - drug therapy ; Glioma - genetics ; Glioma - pathology ; Glioma cells ; Growth factors ; Heterografts ; Humans ; Immunoglobulins ; Mice ; Monoclonal antibodies ; monoclonal antibody ; Peptides ; Proteins ; Short Communication ; Single-Chain Antibodies - immunology ; Single-Chain Antibodies - pharmacology ; single‐chain variable fragment ; Statistical analysis ; Tumors</subject><ispartof>Journal of cellular and molecular medicine, 2018-06, Vol.22 (6), p.3259-3263</ispartof><rights>2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.</rights><rights>2018. This work is published under https://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5427-ca78c6438ce7f585f7764ea323dea81787c4b28433978bee3974812e32b8dc8b3</citedby><cites>FETCH-LOGICAL-c5427-ca78c6438ce7f585f7764ea323dea81787c4b28433978bee3974812e32b8dc8b3</cites><orcidid>0000-0002-9455-1182</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980129/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980129/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29575613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Xiao‐Xiao</creatorcontrib><creatorcontrib>Du, Shuang</creatorcontrib><creatorcontrib>Gao, Shi‐Qian</creatorcontrib><creatorcontrib>Chen, Jing‐Ying</creatorcontrib><creatorcontrib>Cao, Ran‐Juan</creatorcontrib><creatorcontrib>Xing, Zhen‐Kai</creatorcontrib><creatorcontrib>Kazim, Alia Rizvi Syeda</creatorcontrib><creatorcontrib>Yu, Hua‐Li</creatorcontrib><creatorcontrib>Zheng, Qing‐Chuan</creatorcontrib><creatorcontrib>Zhu, Xiao‐Juan</creatorcontrib><title>Humanization of fibroblast growth factor 1 single‐chain antibody and validation for its antitumorigenic efficacy in breast cancer and glioma cells</title><title>Journal of cellular and molecular medicine</title><addtitle>J Cell Mol Med</addtitle><description>Single‐chain variable fragment (scFv) antibodies are the smallest immunoglobulins with high antigen‐binding affinity. We have previously reported that fibroblast growth factor 1 played pivotal roles in cancer development and generated a mouse scFv (mscFv1C9) could effectively prohibit cancer cell proliferation in vitro and in vivo. Here, we further humanized this scFv (hscFv1C9) using a structure‐guided complementarity determining region grafting strategy. The purified hscFv1C9 maintained similar antigen‐binding affinity and specificity as mscFv1C9, and it was capable of inhibiting growth of different tumours in vitro and in vivo. These data strongly suggested that hscFv1C9 has antitumour potentials.</description><subject>Affinity</subject><subject>Amino Acid Sequence - genetics</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal, Humanized - immunology</subject><subject>Antibodies, Monoclonal, Humanized - pharmacology</subject><subject>antibody humanization</subject><subject>Antigens</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer</subject><subject>cancer treatment</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Complementarity</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fibroblast growth factor 1</subject><subject>Fibroblast Growth Factor 1 - antagonists & inhibitors</subject><subject>Fibroblast Growth Factor 1 - chemistry</subject><subject>Fibroblast Growth Factor 1 - genetics</subject><subject>Fibroblast Growth Factor 1 - immunology</subject><subject>Fibroblasts</subject><subject>Glioma</subject><subject>Glioma - drug therapy</subject><subject>Glioma - genetics</subject><subject>Glioma - pathology</subject><subject>Glioma cells</subject><subject>Growth factors</subject><subject>Heterografts</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>monoclonal antibody</subject><subject>Peptides</subject><subject>Proteins</subject><subject>Short Communication</subject><subject>Single-Chain Antibodies - immunology</subject><subject>Single-Chain Antibodies - pharmacology</subject><subject>single‐chain variable fragment</subject><subject>Statistical analysis</subject><subject>Tumors</subject><issn>1582-1838</issn><issn>1582-4934</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9ks9u1DAQhyMEoqVw4QGQJS4IaUv8J7FzQUIraEGtuMDZcpxx1qvELrbTajnxCBx4wj4JzmapgEN98Ujz-fOM9CuK57g8xfm82epxPMW0YvxBcYwrQVasoezhocaCiqPiSYzbsqQ1ps3j4og0Fa9yfVz8Op9G5ex3lax3yBtkbBt8O6iYUB_8Tdogo3TyAWEUresHuP3xU2-UdUi5ZFvf7XLRoWs12G6RmAzbFPf9NI0-2B6c1QiMsVrpHcpv2wDzD1o5DWEv6AfrR4U0DEN8Wjwyaojw7HCfFF8_vP-yPl9dfD77uH53sdIVI3ylFRe6ZlRo4KYSleG8ZqAooR0ogbngmrVEMEobLlqAfDGBCVDSik6Llp4Ubxfv1dSO0GlwKahBXgU7qrCTXln5b8fZjez9tawaUWLSZMGrgyD4bxPEJEcb5xWUAz9FSUos6prTEmf05X_o1k_B5fUkYZwRnOct76XKGatFU2fq9ULp4GMMYO5GxqWcIyHnSMh9JDL84u8l79A_GcgAXoAbO8DuHpX8tL68XKS_AXPKxLE</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>He, Xiao‐Xiao</creator><creator>Du, Shuang</creator><creator>Gao, Shi‐Qian</creator><creator>Chen, Jing‐Ying</creator><creator>Cao, Ran‐Juan</creator><creator>Xing, Zhen‐Kai</creator><creator>Kazim, Alia Rizvi Syeda</creator><creator>Yu, Hua‐Li</creator><creator>Zheng, Qing‐Chuan</creator><creator>Zhu, Xiao‐Juan</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9455-1182</orcidid></search><sort><creationdate>201806</creationdate><title>Humanization of fibroblast growth factor 1 single‐chain antibody and validation for its antitumorigenic efficacy in breast cancer and glioma cells</title><author>He, Xiao‐Xiao ; Du, Shuang ; Gao, Shi‐Qian ; Chen, Jing‐Ying ; Cao, Ran‐Juan ; Xing, Zhen‐Kai ; Kazim, Alia Rizvi Syeda ; Yu, Hua‐Li ; Zheng, Qing‐Chuan ; Zhu, Xiao‐Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5427-ca78c6438ce7f585f7764ea323dea81787c4b28433978bee3974812e32b8dc8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Affinity</topic><topic>Amino Acid Sequence - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cellular and molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Xiao‐Xiao</au><au>Du, Shuang</au><au>Gao, Shi‐Qian</au><au>Chen, Jing‐Ying</au><au>Cao, Ran‐Juan</au><au>Xing, Zhen‐Kai</au><au>Kazim, Alia Rizvi Syeda</au><au>Yu, Hua‐Li</au><au>Zheng, Qing‐Chuan</au><au>Zhu, Xiao‐Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Humanization of fibroblast growth factor 1 single‐chain antibody and validation for its antitumorigenic efficacy in breast cancer and glioma cells</atitle><jtitle>Journal of cellular and molecular medicine</jtitle><addtitle>J Cell Mol Med</addtitle><date>2018-06</date><risdate>2018</risdate><volume>22</volume><issue>6</issue><spage>3259</spage><epage>3263</epage><pages>3259-3263</pages><issn>1582-1838</issn><eissn>1582-4934</eissn><abstract>Single‐chain variable fragment (scFv) antibodies are the smallest immunoglobulins with high antigen‐binding affinity. We have previously reported that fibroblast growth factor 1 played pivotal roles in cancer development and generated a mouse scFv (mscFv1C9) could effectively prohibit cancer cell proliferation in vitro and in vivo. Here, we further humanized this scFv (hscFv1C9) using a structure‐guided complementarity determining region grafting strategy. The purified hscFv1C9 maintained similar antigen‐binding affinity and specificity as mscFv1C9, and it was capable of inhibiting growth of different tumours in vitro and in vivo. These data strongly suggested that hscFv1C9 has antitumour potentials.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>29575613</pmid><doi>10.1111/jcmm.13547</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-9455-1182</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Affinity Amino Acid Sequence - genetics Amino acids Animals Antibodies Antibodies, Monoclonal, Humanized - immunology Antibodies, Monoclonal, Humanized - pharmacology antibody humanization Antigens Breast cancer Breast Neoplasms - drug therapy Breast Neoplasms - genetics Breast Neoplasms - pathology Cancer cancer treatment Cell cycle Cell division Cell growth Cell Line, Tumor Cell proliferation Cell Proliferation - drug effects Complementarity Enzyme-Linked Immunosorbent Assay Female Fibroblast growth factor 1 Fibroblast Growth Factor 1 - antagonists & inhibitors Fibroblast Growth Factor 1 - chemistry Fibroblast Growth Factor 1 - genetics Fibroblast Growth Factor 1 - immunology Fibroblasts Glioma Glioma - drug therapy Glioma - genetics Glioma - pathology Glioma cells Growth factors Heterografts Humans Immunoglobulins Mice Monoclonal antibodies monoclonal antibody Peptides Proteins Short Communication Single-Chain Antibodies - immunology Single-Chain Antibodies - pharmacology single‐chain variable fragment Statistical analysis Tumors |
| title | Humanization of fibroblast growth factor 1 single‐chain antibody and validation for its antitumorigenic efficacy in breast cancer and glioma cells |
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