Biomechanical, Biochemical, and Cell Biological Evaluation of Different Collagen Scaffolds for Tendon Augmentation
Tendon augmentation is increasingly clinically relevant due to rising amount of tendon ruptures because of the aging and more demanding population. Therefore, newly developed scaffolds based on bovine epoxide stabilized collagen maintaining the native fibril-like collagen structure were characterize...
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| Veröffentlicht in: | BioMed research international 2018-01, Vol.2018 (2018), p.1-11 |
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| creator | Bader, Rainer Tischer, Thomas Spohn, Juliane Gabler, Carolin |
| description | Tendon augmentation is increasingly clinically relevant due to rising amount of tendon ruptures because of the aging and more demanding population. Therefore, newly developed scaffolds based on bovine epoxide stabilized collagen maintaining the native fibril-like collagen structure were characterized and compared to two commercially available porcine collagen scaffolds. For biomechanical testing (ultimate load, ultimate stress, stiffness, and elastic modulus), bovine collagen scaffolds were hydrated and compared to reference products. Cell viability and proliferation were assessed by seeding human primary fibroblasts on each collagen-based scaffold and cultured over various time periods (3 d, 7 d, and 14 d). Live/dead staining was performed and metabolic cell activity (WST-1 assay) was measured. Biochemical degradability was investigated by enzymatic digestion. The bovine collagen scaffold showed significantly enhanced biomechanical properties. These persisted over different rehydration times. Cell biological tests revealed that the bovine collagen scaffolds support reproducible cell colonization and a significant increase in the number of viable cells during cultivation. The results are comparable with the viability and proliferation rate of cells grown on porcine reference materials. With regard to biochemical degradability, all tested materials showed comparable resistance to enzymatic degradation in vitro. Due to imitating the natural tendon structure the new scaffold material is supposed to provide beneficial effects in future clinical application. |
| doi_str_mv | 10.1155/2018/7246716 |
| format | Article |
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Therefore, newly developed scaffolds based on bovine epoxide stabilized collagen maintaining the native fibril-like collagen structure were characterized and compared to two commercially available porcine collagen scaffolds. For biomechanical testing (ultimate load, ultimate stress, stiffness, and elastic modulus), bovine collagen scaffolds were hydrated and compared to reference products. Cell viability and proliferation were assessed by seeding human primary fibroblasts on each collagen-based scaffold and cultured over various time periods (3 d, 7 d, and 14 d). Live/dead staining was performed and metabolic cell activity (WST-1 assay) was measured. Biochemical degradability was investigated by enzymatic digestion. The bovine collagen scaffold showed significantly enhanced biomechanical properties. These persisted over different rehydration times. Cell biological tests revealed that the bovine collagen scaffolds support reproducible cell colonization and a significant increase in the number of viable cells during cultivation. The results are comparable with the viability and proliferation rate of cells grown on porcine reference materials. With regard to biochemical degradability, all tested materials showed comparable resistance to enzymatic degradation in vitro. Due to imitating the natural tendon structure the new scaffold material is supposed to provide beneficial effects in future clinical application.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2018/7246716</identifier><identifier>PMID: 29854782</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Aging ; Aging (natural) ; Animals ; Augmentation ; Biochemistry ; Biocompatibility ; Biocompatible Materials - chemistry ; Biomechanics ; Biomedical materials ; Biopsy ; Cattle ; Cell proliferation ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Collagen ; Collagen - chemistry ; Collagen - metabolism ; Colonization ; Comparative analysis ; Cultivation ; Degradability ; Degradation ; Elastic Modulus - drug effects ; Enzymes ; Ethylenediaminetetraacetic acid ; Fibroblasts ; Fibroblasts - chemistry ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Humans ; Joint surgery ; Manufacturing ; Materials Testing - methods ; Mechanical properties ; Medical equipment ; Modulus of elasticity ; Reference materials ; Rehydration ; Scaffolds ; Skin ; Stiffness ; Surgery ; Swine ; Tendon Injuries - metabolism ; Tendon Injuries - therapy ; Tendons - drug effects ; Tensile Strength - drug effects ; Tensile stress ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; Ultimate loads</subject><ispartof>BioMed research international, 2018-01, Vol.2018 (2018), p.1-11</ispartof><rights>Copyright © 2018 Carolin Gabler et al.</rights><rights>COPYRIGHT 2018 John Wiley & Sons, Inc.</rights><rights>Copyright © 2018 Carolin Gabler et al.; This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2018 Carolin Gabler et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-6152b2efcf9bdc70da4173ba6ca5d6113620825928793b5c71fc41528fb0d0833</citedby><cites>FETCH-LOGICAL-c499t-6152b2efcf9bdc70da4173ba6ca5d6113620825928793b5c71fc41528fb0d0833</cites><orcidid>0000-0001-8590-0507 ; 0000-0002-9157-1858</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5966701/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5966701/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29854782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kumar, Pradeep</contributor><creatorcontrib>Bader, Rainer</creatorcontrib><creatorcontrib>Tischer, Thomas</creatorcontrib><creatorcontrib>Spohn, Juliane</creatorcontrib><creatorcontrib>Gabler, Carolin</creatorcontrib><title>Biomechanical, Biochemical, and Cell Biological Evaluation of Different Collagen Scaffolds for Tendon Augmentation</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Tendon augmentation is increasingly clinically relevant due to rising amount of tendon ruptures because of the aging and more demanding population. Therefore, newly developed scaffolds based on bovine epoxide stabilized collagen maintaining the native fibril-like collagen structure were characterized and compared to two commercially available porcine collagen scaffolds. For biomechanical testing (ultimate load, ultimate stress, stiffness, and elastic modulus), bovine collagen scaffolds were hydrated and compared to reference products. Cell viability and proliferation were assessed by seeding human primary fibroblasts on each collagen-based scaffold and cultured over various time periods (3 d, 7 d, and 14 d). Live/dead staining was performed and metabolic cell activity (WST-1 assay) was measured. Biochemical degradability was investigated by enzymatic digestion. The bovine collagen scaffold showed significantly enhanced biomechanical properties. These persisted over different rehydration times. Cell biological tests revealed that the bovine collagen scaffolds support reproducible cell colonization and a significant increase in the number of viable cells during cultivation. The results are comparable with the viability and proliferation rate of cells grown on porcine reference materials. With regard to biochemical degradability, all tested materials showed comparable resistance to enzymatic degradation in vitro. Due to imitating the natural tendon structure the new scaffold material is supposed to provide beneficial effects in future clinical application.</description><subject>Aging</subject><subject>Aging (natural)</subject><subject>Animals</subject><subject>Augmentation</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biomechanics</subject><subject>Biomedical materials</subject><subject>Biopsy</subject><subject>Cattle</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>Collagen - chemistry</subject><subject>Collagen - metabolism</subject><subject>Colonization</subject><subject>Comparative analysis</subject><subject>Cultivation</subject><subject>Degradability</subject><subject>Degradation</subject><subject>Elastic Modulus - drug effects</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fibroblasts</subject><subject>Fibroblasts - chemistry</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Joint surgery</subject><subject>Manufacturing</subject><subject>Materials Testing - methods</subject><subject>Mechanical properties</subject><subject>Medical equipment</subject><subject>Modulus of elasticity</subject><subject>Reference materials</subject><subject>Rehydration</subject><subject>Scaffolds</subject><subject>Skin</subject><subject>Stiffness</subject><subject>Surgery</subject><subject>Swine</subject><subject>Tendon Injuries - metabolism</subject><subject>Tendon Injuries - therapy</subject><subject>Tendons - drug effects</subject><subject>Tensile Strength - drug effects</subject><subject>Tensile stress</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Ultimate loads</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkc9vFCEUx4nR2Kb25tlM4sXEruX3wMVku60_kiYerGfCMDBLw0CFnRr_e5nOulVPcoH3-Lzve_AF4CWC7xBi7BxDJM5bTHmL-BNwjAmiK44oeno4E3IETku5hXUJxKHkz8ERloLRVuBjkC98Gq3Z6uiNDmdNDc3WjkugY99sbAhzNqRhTjZX9zpMeudTbJJrLr1zNtu4azYpBD3Y2Hw12rkU-tK4lJsbG_uKrqdhrNRD3QvwzOlQ7Ol-PwHfPlzdbD6trr98_LxZX68MlXJXR2e4w9YZJ7vetLDXFLWk09xo1nOECMdQYCaxaCXpmGmRM7TWCNfBHgpCTsD7Rfdu6kbbm9o_66Dush91_qmS9urvm-i3akj3iknOW4iqwJu9QE7fJ1t2avTF1P_Q0aapKAypZAxRjiv6-h_0Nk051ufNVHVK4pY_UoMOVvnoUu1rZlG15hgJCJe2ZwtlciolW3cYGUE1u65m19Xe9Yq_-vOZB_i3xxV4uwBbH3v9w_-nnK2MdfqRRlgQRskv_xi9Fg</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Bader, Rainer</creator><creator>Tischer, Thomas</creator><creator>Spohn, Juliane</creator><creator>Gabler, Carolin</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8590-0507</orcidid><orcidid>https://orcid.org/0000-0002-9157-1858</orcidid></search><sort><creationdate>20180101</creationdate><title>Biomechanical, Biochemical, and Cell Biological Evaluation of Different Collagen Scaffolds for Tendon Augmentation</title><author>Bader, Rainer ; Tischer, Thomas ; Spohn, Juliane ; Gabler, Carolin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-6152b2efcf9bdc70da4173ba6ca5d6113620825928793b5c71fc41528fb0d0833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aging</topic><topic>Aging (natural)</topic><topic>Animals</topic><topic>Augmentation</topic><topic>Biochemistry</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biomechanics</topic><topic>Biomedical materials</topic><topic>Biopsy</topic><topic>Cattle</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Collagen</topic><topic>Collagen - chemistry</topic><topic>Collagen - metabolism</topic><topic>Colonization</topic><topic>Comparative analysis</topic><topic>Cultivation</topic><topic>Degradability</topic><topic>Degradation</topic><topic>Elastic Modulus - drug effects</topic><topic>Enzymes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Fibroblasts</topic><topic>Fibroblasts - chemistry</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Joint surgery</topic><topic>Manufacturing</topic><topic>Materials Testing - methods</topic><topic>Mechanical properties</topic><topic>Medical equipment</topic><topic>Modulus of elasticity</topic><topic>Reference materials</topic><topic>Rehydration</topic><topic>Scaffolds</topic><topic>Skin</topic><topic>Stiffness</topic><topic>Surgery</topic><topic>Swine</topic><topic>Tendon Injuries - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bader, Rainer</au><au>Tischer, Thomas</au><au>Spohn, Juliane</au><au>Gabler, Carolin</au><au>Kumar, Pradeep</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biomechanical, Biochemical, and Cell Biological Evaluation of Different Collagen Scaffolds for Tendon Augmentation</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>2018</volume><issue>2018</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Tendon augmentation is increasingly clinically relevant due to rising amount of tendon ruptures because of the aging and more demanding population. Therefore, newly developed scaffolds based on bovine epoxide stabilized collagen maintaining the native fibril-like collagen structure were characterized and compared to two commercially available porcine collagen scaffolds. For biomechanical testing (ultimate load, ultimate stress, stiffness, and elastic modulus), bovine collagen scaffolds were hydrated and compared to reference products. Cell viability and proliferation were assessed by seeding human primary fibroblasts on each collagen-based scaffold and cultured over various time periods (3 d, 7 d, and 14 d). Live/dead staining was performed and metabolic cell activity (WST-1 assay) was measured. Biochemical degradability was investigated by enzymatic digestion. The bovine collagen scaffold showed significantly enhanced biomechanical properties. These persisted over different rehydration times. Cell biological tests revealed that the bovine collagen scaffolds support reproducible cell colonization and a significant increase in the number of viable cells during cultivation. The results are comparable with the viability and proliferation rate of cells grown on porcine reference materials. With regard to biochemical degradability, all tested materials showed comparable resistance to enzymatic degradation in vitro. Due to imitating the natural tendon structure the new scaffold material is supposed to provide beneficial effects in future clinical application.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>29854782</pmid><doi>10.1155/2018/7246716</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8590-0507</orcidid><orcidid>https://orcid.org/0000-0002-9157-1858</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aging Aging (natural) Animals Augmentation Biochemistry Biocompatibility Biocompatible Materials - chemistry Biomechanics Biomedical materials Biopsy Cattle Cell proliferation Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Collagen Collagen - chemistry Collagen - metabolism Colonization Comparative analysis Cultivation Degradability Degradation Elastic Modulus - drug effects Enzymes Ethylenediaminetetraacetic acid Fibroblasts Fibroblasts - chemistry Fibroblasts - drug effects Fibroblasts - metabolism Humans Joint surgery Manufacturing Materials Testing - methods Mechanical properties Medical equipment Modulus of elasticity Reference materials Rehydration Scaffolds Skin Stiffness Surgery Swine Tendon Injuries - metabolism Tendon Injuries - therapy Tendons - drug effects Tensile Strength - drug effects Tensile stress Tissue engineering Tissue Engineering - methods Tissue Scaffolds - chemistry Ultimate loads |
| title | Biomechanical, Biochemical, and Cell Biological Evaluation of Different Collagen Scaffolds for Tendon Augmentation |
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