Quantum-Optically Enhanced STORM (QUEST) for Multi-Emitter Localization
Super-resolution imaging has introduced new capabilities to investigate processes at the nanometer scale by optical means. However, most super-resolution techniques require either sparse excitation of few emitters or analysis of high-order cumulants in order to identify several emitters in close vic...
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| Veröffentlicht in: | Scientific reports 2018-05, Vol.8 (1), p.7829-12, Article 7829 |
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| description | Super-resolution imaging has introduced new capabilities to investigate processes at the nanometer scale by optical means. However, most super-resolution techniques require either sparse excitation of few emitters or analysis of high-order cumulants in order to identify several emitters in close vicinity. Here, we present an approach that draws upon methods from quantum optics to perform localization super-resolution imaging of densely packed emitters and determine their number automatically: Quantum-optically enhanced STORM (QUEST). By exploiting normalized photon correlations, we predict a localization precision below 30 nm or better even for closely spaced emitter up to a density of 125 emitters per
μ
m at photon emission rates of 10
5
photons per second and emitter. Our technique does not require complex experimental arrangements and relies solely on spatially resolved time streams of photons and subsequent data analysis. |
| doi_str_mv | 10.1038/s41598-018-26271-1 |
| format | Article |
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μ
m at photon emission rates of 10
5
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μ
m at photon emission rates of 10
5
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μ
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5
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| subjects | 140/125 639/624/1107/328/2238 639/624/400/482 Data processing Humanities and Social Sciences Localization multidisciplinary Optics Photons Science Science (multidisciplinary) |
| title | Quantum-Optically Enhanced STORM (QUEST) for Multi-Emitter Localization |
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