Genetic Diversity Analysis Reveals Genetic Differentiation and Strong Population Structure in Calotropis Plants
The genus Calotropis (Asclepiadaceae) is comprised of two species, C . gigantea and C . procera , which both show significant economic potential for use of their seed fibers in the textile industry, and of their bioactive compounds as new medicinal resources. The available wild-sourced germplasm con...
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| Veröffentlicht in: | Scientific reports 2018-05, Vol.8 (1), p.7832-10, Article 7832 |
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| creator | Muriira, Nkatha G. Muchugi, Alice Yu, Anmin Xu, Jianchu Liu, Aizhong |
| description | The genus
Calotropis
(Asclepiadaceae) is comprised of two species,
C
.
gigantea
and
C
.
procera
, which both show significant economic potential for use of their seed fibers in the textile industry, and of their bioactive compounds as new medicinal resources. The available wild-sourced germplasm contains limited genetic information that restricts further germplasm exploration for the purposes of domestication. We here developed twenty novel EST-SSR markers and applied them to assess genetic diversity, population structure and differentiation within
Calotropis
. The polymorphic information index of these markers ranged from 0.102 to 0.800; indicating that they are highly informative. Moderate genetic diversity was revealed in both species, with no difference between species in the amount of genetic diversity. Population structure analysis suggested five main genetic groups (K = 5) and relatively high genetic differentiation (
F
ST
= 0.528) between the two species. Mantel test analysis showed strong correlation between geographical and genetic distance in
C
.
procera
(r = 0.875, p = 0.020) while
C
.
gigantea
showed no such correlation (r = 0.390, p = 0.210). This study provides novel insights into the genetic diversity and population structure of
Calotropis
, which will promote further resource utilization and the development of genetic improvement strategies for
Calotropis
. |
| doi_str_mv | 10.1038/s41598-018-26275-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5959898</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2041624569</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-ddfb5edd7336ec21b0f741af91a014c20febf4d6f636f112ef5e0701c99e40dd3</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhi0EolXpH-CALHHhEvA4cRJfkKotFKRKVHycLa89Xlxl7cV2Vt1_jyGlLRzwxdbMM69n5iXkObDXwNrxTe5AyLFhMDa854Nobh6RY8460fCW88cP3kfkNOdrVo_gsgP5lBxxOQwD9HBM4gUGLN7Qc7_HlH050LOgp0P2mX7GPeop03vEOUwYitfFx0B1sPRLSTFs6FXczdMSrZHZlDkh9YGu9BQrsatqV5MOJT8jT1zVxNPb-4R8e__u6-pDc_np4uPq7LIxAqA01rq1QGuHtu3RcFgzN3SgnQTNoDOcOVy7zvaub3sHwNEJZAMDIyV2zNr2hLxddHfzeovW1K6TntQu-a1OBxW1V39ngv-uNnGvhKxrlWMVeHUrkOKPGXNRW58NTnUKjHNWdb3Q8070sqIv_0Gv45zqFhcKWiZGqBRfKJNizgndXTPA1C9L1WKpqpaq35aqm1r04uEYdyV_DKxAuwC5psIG0_3f_5H9CUPfsB4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2041130581</pqid></control><display><type>article</type><title>Genetic Diversity Analysis Reveals Genetic Differentiation and Strong Population Structure in Calotropis Plants</title><source>MEDLINE</source><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Muriira, Nkatha G. ; Muchugi, Alice ; Yu, Anmin ; Xu, Jianchu ; Liu, Aizhong</creator><creatorcontrib>Muriira, Nkatha G. ; Muchugi, Alice ; Yu, Anmin ; Xu, Jianchu ; Liu, Aizhong</creatorcontrib><description>The genus
Calotropis
(Asclepiadaceae) is comprised of two species,
C
.
gigantea
and
C
.
procera
, which both show significant economic potential for use of their seed fibers in the textile industry, and of their bioactive compounds as new medicinal resources. The available wild-sourced germplasm contains limited genetic information that restricts further germplasm exploration for the purposes of domestication. We here developed twenty novel EST-SSR markers and applied them to assess genetic diversity, population structure and differentiation within
Calotropis
. The polymorphic information index of these markers ranged from 0.102 to 0.800; indicating that they are highly informative. Moderate genetic diversity was revealed in both species, with no difference between species in the amount of genetic diversity. Population structure analysis suggested five main genetic groups (K = 5) and relatively high genetic differentiation (
F
ST
= 0.528) between the two species. Mantel test analysis showed strong correlation between geographical and genetic distance in
C
.
procera
(r = 0.875, p = 0.020) while
C
.
gigantea
showed no such correlation (r = 0.390, p = 0.210). This study provides novel insights into the genetic diversity and population structure of
Calotropis
, which will promote further resource utilization and the development of genetic improvement strategies for
Calotropis
.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-26275-x</identifier><identifier>PMID: 29777161</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>101/47 ; 38/71 ; 38/77 ; 45/29 ; 631/449 ; 631/449/2491 ; Bioactive compounds ; Calotropis - genetics ; Calotropis procera ; Databases, Genetic ; DNA, Plant - genetics ; Domestication ; Evolution, Molecular ; Exploration ; Expressed Sequence Tags ; Fibers ; Gene Expression Profiling - methods ; Genetic analysis ; Genetic distance ; Genetic diversity ; Genetic Drift ; Genetic Markers ; Genetic Variation ; Genetics, Population ; Genotype & phenotype ; Germplasm ; Humanities and Social Sciences ; multidisciplinary ; Population ; Population genetics ; Population structure ; Resource utilization ; Science ; Science (multidisciplinary) ; Species ; Textile industry</subject><ispartof>Scientific reports, 2018-05, Vol.8 (1), p.7832-10, Article 7832</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-ddfb5edd7336ec21b0f741af91a014c20febf4d6f636f112ef5e0701c99e40dd3</citedby><cites>FETCH-LOGICAL-c511t-ddfb5edd7336ec21b0f741af91a014c20febf4d6f636f112ef5e0701c99e40dd3</cites><orcidid>0000-0002-3197-5535</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5959898/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5959898/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29777161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muriira, Nkatha G.</creatorcontrib><creatorcontrib>Muchugi, Alice</creatorcontrib><creatorcontrib>Yu, Anmin</creatorcontrib><creatorcontrib>Xu, Jianchu</creatorcontrib><creatorcontrib>Liu, Aizhong</creatorcontrib><title>Genetic Diversity Analysis Reveals Genetic Differentiation and Strong Population Structure in Calotropis Plants</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The genus
Calotropis
(Asclepiadaceae) is comprised of two species,
C
.
gigantea
and
C
.
procera
, which both show significant economic potential for use of their seed fibers in the textile industry, and of their bioactive compounds as new medicinal resources. The available wild-sourced germplasm contains limited genetic information that restricts further germplasm exploration for the purposes of domestication. We here developed twenty novel EST-SSR markers and applied them to assess genetic diversity, population structure and differentiation within
Calotropis
. The polymorphic information index of these markers ranged from 0.102 to 0.800; indicating that they are highly informative. Moderate genetic diversity was revealed in both species, with no difference between species in the amount of genetic diversity. Population structure analysis suggested five main genetic groups (K = 5) and relatively high genetic differentiation (
F
ST
= 0.528) between the two species. Mantel test analysis showed strong correlation between geographical and genetic distance in
C
.
procera
(r = 0.875, p = 0.020) while
C
.
gigantea
showed no such correlation (r = 0.390, p = 0.210). This study provides novel insights into the genetic diversity and population structure of
Calotropis
, which will promote further resource utilization and the development of genetic improvement strategies for
Calotropis
.</description><subject>101/47</subject><subject>38/71</subject><subject>38/77</subject><subject>45/29</subject><subject>631/449</subject><subject>631/449/2491</subject><subject>Bioactive compounds</subject><subject>Calotropis - genetics</subject><subject>Calotropis procera</subject><subject>Databases, Genetic</subject><subject>DNA, Plant - genetics</subject><subject>Domestication</subject><subject>Evolution, Molecular</subject><subject>Exploration</subject><subject>Expressed Sequence Tags</subject><subject>Fibers</subject><subject>Gene Expression Profiling - methods</subject><subject>Genetic analysis</subject><subject>Genetic distance</subject><subject>Genetic diversity</subject><subject>Genetic Drift</subject><subject>Genetic Markers</subject><subject>Genetic Variation</subject><subject>Genetics, Population</subject><subject>Genotype & phenotype</subject><subject>Germplasm</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Population</subject><subject>Population genetics</subject><subject>Population structure</subject><subject>Resource utilization</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Species</subject><subject>Textile industry</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1v1DAQhi0EolXpH-CALHHhEvA4cRJfkKotFKRKVHycLa89Xlxl7cV2Vt1_jyGlLRzwxdbMM69n5iXkObDXwNrxTe5AyLFhMDa854Nobh6RY8460fCW88cP3kfkNOdrVo_gsgP5lBxxOQwD9HBM4gUGLN7Qc7_HlH050LOgp0P2mX7GPeop03vEOUwYitfFx0B1sPRLSTFs6FXczdMSrZHZlDkh9YGu9BQrsatqV5MOJT8jT1zVxNPb-4R8e__u6-pDc_np4uPq7LIxAqA01rq1QGuHtu3RcFgzN3SgnQTNoDOcOVy7zvaub3sHwNEJZAMDIyV2zNr2hLxddHfzeovW1K6TntQu-a1OBxW1V39ngv-uNnGvhKxrlWMVeHUrkOKPGXNRW58NTnUKjHNWdb3Q8070sqIv_0Gv45zqFhcKWiZGqBRfKJNizgndXTPA1C9L1WKpqpaq35aqm1r04uEYdyV_DKxAuwC5psIG0_3f_5H9CUPfsB4</recordid><startdate>20180518</startdate><enddate>20180518</enddate><creator>Muriira, Nkatha G.</creator><creator>Muchugi, Alice</creator><creator>Yu, Anmin</creator><creator>Xu, Jianchu</creator><creator>Liu, Aizhong</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3197-5535</orcidid></search><sort><creationdate>20180518</creationdate><title>Genetic Diversity Analysis Reveals Genetic Differentiation and Strong Population Structure in Calotropis Plants</title><author>Muriira, Nkatha G. ; Muchugi, Alice ; Yu, Anmin ; Xu, Jianchu ; Liu, Aizhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-ddfb5edd7336ec21b0f741af91a014c20febf4d6f636f112ef5e0701c99e40dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>101/47</topic><topic>38/71</topic><topic>38/77</topic><topic>45/29</topic><topic>631/449</topic><topic>631/449/2491</topic><topic>Bioactive compounds</topic><topic>Calotropis - genetics</topic><topic>Calotropis procera</topic><topic>Databases, Genetic</topic><topic>DNA, Plant - genetics</topic><topic>Domestication</topic><topic>Evolution, Molecular</topic><topic>Exploration</topic><topic>Expressed Sequence Tags</topic><topic>Fibers</topic><topic>Gene Expression Profiling - methods</topic><topic>Genetic analysis</topic><topic>Genetic distance</topic><topic>Genetic diversity</topic><topic>Genetic Drift</topic><topic>Genetic Markers</topic><topic>Genetic Variation</topic><topic>Genetics, Population</topic><topic>Genotype & phenotype</topic><topic>Germplasm</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Population</topic><topic>Population genetics</topic><topic>Population structure</topic><topic>Resource utilization</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Species</topic><topic>Textile industry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muriira, Nkatha G.</creatorcontrib><creatorcontrib>Muchugi, Alice</creatorcontrib><creatorcontrib>Yu, Anmin</creatorcontrib><creatorcontrib>Xu, Jianchu</creatorcontrib><creatorcontrib>Liu, Aizhong</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muriira, Nkatha G.</au><au>Muchugi, Alice</au><au>Yu, Anmin</au><au>Xu, Jianchu</au><au>Liu, Aizhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Diversity Analysis Reveals Genetic Differentiation and Strong Population Structure in Calotropis Plants</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-05-18</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>7832</spage><epage>10</epage><pages>7832-10</pages><artnum>7832</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The genus
Calotropis
(Asclepiadaceae) is comprised of two species,
C
.
gigantea
and
C
.
procera
, which both show significant economic potential for use of their seed fibers in the textile industry, and of their bioactive compounds as new medicinal resources. The available wild-sourced germplasm contains limited genetic information that restricts further germplasm exploration for the purposes of domestication. We here developed twenty novel EST-SSR markers and applied them to assess genetic diversity, population structure and differentiation within
Calotropis
. The polymorphic information index of these markers ranged from 0.102 to 0.800; indicating that they are highly informative. Moderate genetic diversity was revealed in both species, with no difference between species in the amount of genetic diversity. Population structure analysis suggested five main genetic groups (K = 5) and relatively high genetic differentiation (
F
ST
= 0.528) between the two species. Mantel test analysis showed strong correlation between geographical and genetic distance in
C
.
procera
(r = 0.875, p = 0.020) while
C
.
gigantea
showed no such correlation (r = 0.390, p = 0.210). This study provides novel insights into the genetic diversity and population structure of
Calotropis
, which will promote further resource utilization and the development of genetic improvement strategies for
Calotropis
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29777161</pmid><doi>10.1038/s41598-018-26275-x</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-3197-5535</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 101/47 38/71 38/77 45/29 631/449 631/449/2491 Bioactive compounds Calotropis - genetics Calotropis procera Databases, Genetic DNA, Plant - genetics Domestication Evolution, Molecular Exploration Expressed Sequence Tags Fibers Gene Expression Profiling - methods Genetic analysis Genetic distance Genetic diversity Genetic Drift Genetic Markers Genetic Variation Genetics, Population Genotype & phenotype Germplasm Humanities and Social Sciences multidisciplinary Population Population genetics Population structure Resource utilization Science Science (multidisciplinary) Species Textile industry |
| title | Genetic Diversity Analysis Reveals Genetic Differentiation and Strong Population Structure in Calotropis Plants |
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