Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic gen...

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Veröffentlicht in:	Molecular cell 2017-08, Vol.67 (4), p.633-645.e3
Hauptverfasser:	Yamano, Takashi, Zetsche, Bernd, Ishitani, Ryuichiro, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu
Format:	Artikel
Sprache:	eng
Schlagworte:	Acidaminococcus Acidaminococcus - enzymology Acidaminococcus - genetics bacteria Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding Sites Cas12a Clostridiales - enzymology Clostridiales - genetics Clustered Regularly Interspaced Short Palindromic Repeats Cpf1 CRISPR-Associated Proteins - chemistry CRISPR-Associated Proteins - genetics CRISPR-Associated Proteins - metabolism CRISPR-Cas CRISPR-Cas Systems crystal structure Crystallography, X-Ray DNA DNA - chemistry DNA - genetics DNA - metabolism DNA damage Endonucleases - chemistry Endonucleases - genetics Endonucleases - metabolism Escherichia coli - enzymology Escherichia coli - genetics gene editing HEK293 Cells Humans Lachnospiraceae Models, Molecular nucleases Nucleic Acid Conformation Nucleic Acid Heteroduplexes - chemistry Nucleic Acid Heteroduplexes - genetics Nucleic Acid Heteroduplexes - metabolism nucleotide sequences Protein Binding Protein Conformation protospacer adjacent motif RNA RNA, Guide, CRISPR-Cas Systems - chemistry RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - metabolism Structure-Activity Relationship
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