$A microfluidic chip with a staircase pH gradient generator, a packed column and a fraction collector for chromatofocusing of proteins$

A microfluidic chip with a staircase pH gradient generator, a packed column and a fraction collector for chromatofocusing of proteins

A microfluidic device for pH gradient chromatofocusing is presented, which performs creation of a micro‐column, pH gradient generation, and fraction collection in a single device. Using a sieve micro‐valve, anion exchange particles were packed into a microchannel in order to realize a solid‐phase ab...

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Veröffentlicht in:	Electrophoresis 2018-04, Vol.39 (8), p.1031-1039
Hauptverfasser:	Rho, Hoon Suk, Hanke, Alexander Thomas, Ottens, Marcel, Gardeniers, Han J.G.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	absorption adsorption anion exchange Anion exchanging Buffer solutions Charged particles Chromatography - methods desorption Diagnostic software Diagnostic systems diagnostic techniques electrophoresis Elution Fluorescein Hydrogen-Ion Concentration Industrial applications Isoelectric Point isothiocyanates Microfluidic Analytical Techniques - instrumentation Microfluidics mixing Nucleic acids Packed columns Part I. Proteins and Proteomics pH gradient chromatofocusing Phycoerythrin - isolation & purification Protein separation Proteins Proteins - analysis Proteins - isolation & purification Scientific apparatus & instruments screening Serum Albumin, Bovine - isolation & purification
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