Global site-specific analysis of glycoprotein N-glycan processing

This protocol describes a semiquantitative glycoproteomics method using sequential treatment with endoglycosidases to create unique mass signatures to determine glycan occupancy and proportion of high-mannose and complex glycans at each glycosite. N-glycans contribute to the folding, stability and f...

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Veröffentlicht in:	Nature protocols 2018-06, Vol.13 (6), p.1196-1212
Hauptverfasser:	Cao, Liwei, Diedrich, Jolene K, Ma, Yuanhui, Wang, Nianshuang, Pauthner, Matthias, Park, Sung-Kyu Robin, Delahunty, Claire M, McLellan, Jason S, Burton, Dennis R, Yates, John R, Paulson, James C
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/296 631/45/1268 631/45/474 631/45/475 631/92/612/1231 82/58 Analytical Chemistry Biological Techniques Computational Biology/Bioinformatics Data analysis Data processing Denaturation Glycan Glycopeptides Glycoproteins Glycosylation Life Sciences Mannose Mass spectrometry Mass spectroscopy Microarrays Mixtures Monosaccharides N-glycans Organic Chemistry Peptides Polysaccharides Properties Protein metabolism Protein research Proteins Proteolysis Proteomics protocol Quantitation Signatures Stability analysis
Online-Zugang:	Volltext
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