Generation of new glutinous rice by CRISPR/Cas9‐targeted mutagenesis of the Waxy gene in elite rice varieties
Summary In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated 9 (Cas9) to introduce a loss‐of‐function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our...
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| Veröffentlicht in: | Journal of integrative plant biology 2018-05, Vol.60 (5), p.369-375 |
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| creator | Zhang, Jinshan Zhang, Hui Botella, José Ramón Zhu, Jian‐Kang |
| description | Summary
In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated 9 (Cas9) to introduce a loss‐of‐function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.
In this study, we reported generation of new glutinous rice with potential for commercialization by editing Waxy gene in elite crop varieties. |
| doi_str_mv | 10.1111/jipb.12620 |
| format | Article |
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In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated 9 (Cas9) to introduce a loss‐of‐function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.
In this study, we reported generation of new glutinous rice with potential for commercialization by editing Waxy gene in elite crop varieties.</description><identifier>ISSN: 1672-9072</identifier><identifier>EISSN: 1744-7909</identifier><identifier>DOI: 10.1111/jipb.12620</identifier><identifier>PMID: 29210506</identifier><language>eng</language><publisher>China (Republic : 1949- ): Wiley Subscription Services, Inc</publisher><subject>agronomic traits ; Agronomy ; Amylose ; Amylose - metabolism ; Base Sequence ; Commercialization ; CRISPR ; CRISPR-Cas Systems - genetics ; Crops ; cultivars ; Endosperm - metabolism ; Endosperm - ultrastructure ; Genes, Plant ; glutinous rice ; loss-of-function mutation ; mutagenesis ; Mutagenesis - genetics ; Mutation ; Mutation - genetics ; Oryza ; Oryza - genetics ; Oryza - ultrastructure ; Phenotype ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Progeny ; Rice ; Site-directed mutagenesis ; Starch - metabolism ; Transgenes ; Waxy gene</subject><ispartof>Journal of integrative plant biology, 2018-05, Vol.60 (5), p.369-375</ispartof><rights>2017 Institute of Botany, Chinese Academy of Sciences</rights><rights>2017 Institute of Botany, Chinese Academy of Sciences.</rights><rights>2018 Institute of Botany, Chinese Academy of Sciences</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6180-ee79f317b24be16a703ada0067d4ef722b1347e42b6c6b661aaaeb7b0ae872b73</citedby><cites>FETCH-LOGICAL-c6180-ee79f317b24be16a703ada0067d4ef722b1347e42b6c6b661aaaeb7b0ae872b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zwxb/zwxb.jpg</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjipb.12620$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjipb.12620$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29210506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Jinshan</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Botella, José Ramón</creatorcontrib><creatorcontrib>Zhu, Jian‐Kang</creatorcontrib><title>Generation of new glutinous rice by CRISPR/Cas9‐targeted mutagenesis of the Waxy gene in elite rice varieties</title><title>Journal of integrative plant biology</title><addtitle>J Integr Plant Biol</addtitle><description>Summary
In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated 9 (Cas9) to introduce a loss‐of‐function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.
In this study, we reported generation of new glutinous rice with potential for commercialization by editing Waxy gene in elite crop varieties.</description><subject>agronomic traits</subject><subject>Agronomy</subject><subject>Amylose</subject><subject>Amylose - metabolism</subject><subject>Base Sequence</subject><subject>Commercialization</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>Crops</subject><subject>cultivars</subject><subject>Endosperm - metabolism</subject><subject>Endosperm - ultrastructure</subject><subject>Genes, Plant</subject><subject>glutinous rice</subject><subject>loss-of-function mutation</subject><subject>mutagenesis</subject><subject>Mutagenesis - genetics</subject><subject>Mutation</subject><subject>Mutation - genetics</subject><subject>Oryza</subject><subject>Oryza - genetics</subject><subject>Oryza - ultrastructure</subject><subject>Phenotype</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Progeny</subject><subject>Rice</subject><subject>Site-directed mutagenesis</subject><subject>Starch - metabolism</subject><subject>Transgenes</subject><subject>Waxy gene</subject><issn>1672-9072</issn><issn>1744-7909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd-K1DAUh4so7h-98QEkIMIidPckbZP2ZkEHXUcWXFbFy5B0TrsZOumYpDs7XvkIPqNPYmrHQb0wNwnJly_n5JckTyic0jjOlmatTynjDO4lh1TkeSoqqO7HNRcsrUCwg-TI-yVAVgJnD5MDVjEKBfDDpL9Ai04F01vSN8TihrTdEIztB0-cqZHoLZldzz9cXZ_NlK9-fPselGsx4IKshqDaeN0bP94NN0g-q7stGfeIsQQ7E3CS3CpnMBj0j5IHjeo8Pt7Nx8mnN68_zt6ml-8v5rOXl2nNaQkpoqiajArNco2UKwGZWigALhY5NoIxTbNcYM40r7nmnCqlUAsNCkvBtMiOk_PJux70Chc12uBUJ9fOrJTbyl4Z-feJNTey7W9lUWUlpTwKnk-CjbKNsq1c9oOzsWT5dXOnGcQqCwAWuZPdQ67_MqAPcmV8jV2nLMY_lAzKHHieixF99g-6dzLICsZEyYpIvZio2vXeO2z2RVOQY95yzFv-yjvCT_9sc4_-DjgCdNeG6XD7H5V8N796NUl_Ak9dtr8</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Zhang, Jinshan</creator><creator>Zhang, Hui</creator><creator>Botella, José Ramón</creator><creator>Zhu, Jian‐Kang</creator><general>Wiley Subscription Services, Inc</general><general>Shanghai Center for Plant Stress Biology, and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China</general><general>University of Chinese Academy of Sciences, Shanghai 201602, China%Shanghai Center for Plant Stress Biology, and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China</general><general>Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA%Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane QLD 4072, Australia</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7S9</scope><scope>L.6</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>201805</creationdate><title>Generation of new glutinous rice by CRISPR/Cas9‐targeted mutagenesis of the Waxy gene in elite rice varieties</title><author>Zhang, Jinshan ; Zhang, Hui ; Botella, José Ramón ; Zhu, Jian‐Kang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6180-ee79f317b24be16a703ada0067d4ef722b1347e42b6c6b661aaaeb7b0ae872b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>agronomic traits</topic><topic>Agronomy</topic><topic>Amylose</topic><topic>Amylose - metabolism</topic><topic>Base Sequence</topic><topic>Commercialization</topic><topic>CRISPR</topic><topic>CRISPR-Cas Systems - genetics</topic><topic>Crops</topic><topic>cultivars</topic><topic>Endosperm - metabolism</topic><topic>Endosperm - ultrastructure</topic><topic>Genes, Plant</topic><topic>glutinous rice</topic><topic>loss-of-function mutation</topic><topic>mutagenesis</topic><topic>Mutagenesis - genetics</topic><topic>Mutation</topic><topic>Mutation - genetics</topic><topic>Oryza</topic><topic>Oryza - genetics</topic><topic>Oryza - ultrastructure</topic><topic>Phenotype</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Progeny</topic><topic>Rice</topic><topic>Site-directed mutagenesis</topic><topic>Starch - metabolism</topic><topic>Transgenes</topic><topic>Waxy gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jinshan</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Botella, José Ramón</creatorcontrib><creatorcontrib>Zhu, Jian‐Kang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of integrative plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jinshan</au><au>Zhang, Hui</au><au>Botella, José Ramón</au><au>Zhu, Jian‐Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of new glutinous rice by CRISPR/Cas9‐targeted mutagenesis of the Waxy gene in elite rice varieties</atitle><jtitle>Journal of integrative plant biology</jtitle><addtitle>J Integr Plant Biol</addtitle><date>2018-05</date><risdate>2018</risdate><volume>60</volume><issue>5</issue><spage>369</spage><epage>375</epage><pages>369-375</pages><issn>1672-9072</issn><eissn>1744-7909</eissn><abstract>Summary
In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated 9 (Cas9) to introduce a loss‐of‐function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.
In this study, we reported generation of new glutinous rice with potential for commercialization by editing Waxy gene in elite crop varieties.</abstract><cop>China (Republic : 1949- )</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29210506</pmid><doi>10.1111/jipb.12620</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | agronomic traits Agronomy Amylose Amylose - metabolism Base Sequence Commercialization CRISPR CRISPR-Cas Systems - genetics Crops cultivars Endosperm - metabolism Endosperm - ultrastructure Genes, Plant glutinous rice loss-of-function mutation mutagenesis Mutagenesis - genetics Mutation Mutation - genetics Oryza Oryza - genetics Oryza - ultrastructure Phenotype Plant Proteins - genetics Plant Proteins - metabolism Progeny Rice Site-directed mutagenesis Starch - metabolism Transgenes Waxy gene |
| title | Generation of new glutinous rice by CRISPR/Cas9‐targeted mutagenesis of the Waxy gene in elite rice varieties |
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