MicroRNA Expression Profiling in Psoriatic Arthritis
Background. Psoriatic arthritis (PsA) is an inflammatory arthritis, characterized by bone erosions and new bone formation. MicroRNAs (miRNAs) are key regulators of the immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their ro...
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| description | Background. Psoriatic arthritis (PsA) is an inflammatory arthritis, characterized by bone erosions and new bone formation. MicroRNAs (miRNAs) are key regulators of the immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their role in PsA is not fully elucidated. We aimed to identify miRNA expression signatures associated with PsA and to investigate their potential implication in the disease pathogenesis. Methods. miRNA microarray was performed in blood cells of PsA patients and healthy controls. miRNA pathway analyses were performed and the global miRNA profiling was combined with transcriptome data in PsA. Deregulation of selected miRNAs was validated by real-time PCR. Results. We identified specific miRNA signatures associated with PsA patients with active disease. These miRNAs target pathways relevant in PsA, such as TNF, MAPK, and WNT signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the PsA transcriptome. miR-126-3p was the most downregulated miRNA in active patients. Noteworthy, miR-126 overexpression induced a decreased expression of genes implicated in PsA. Conclusions. This study sheds light on some epigenetic aspects of PsA identifying specific miRNAs, which may represent promising candidates as biomarkers and/or for the design of novel therapeutic strategies in PsA. |
| doi_str_mv | 10.1155/2018/7305380 |
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Psoriatic arthritis (PsA) is an inflammatory arthritis, characterized by bone erosions and new bone formation. MicroRNAs (miRNAs) are key regulators of the immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their role in PsA is not fully elucidated. We aimed to identify miRNA expression signatures associated with PsA and to investigate their potential implication in the disease pathogenesis. Methods. miRNA microarray was performed in blood cells of PsA patients and healthy controls. miRNA pathway analyses were performed and the global miRNA profiling was combined with transcriptome data in PsA. Deregulation of selected miRNAs was validated by real-time PCR. Results. We identified specific miRNA signatures associated with PsA patients with active disease. These miRNAs target pathways relevant in PsA, such as TNF, MAPK, and WNT signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the PsA transcriptome. miR-126-3p was the most downregulated miRNA in active patients. Noteworthy, miR-126 overexpression induced a decreased expression of genes implicated in PsA. Conclusions. This study sheds light on some epigenetic aspects of PsA identifying specific miRNAs, which may represent promising candidates as biomarkers and/or for the design of novel therapeutic strategies in PsA.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2018/7305380</identifier><identifier>PMID: 29850558</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adult ; Arthritis ; Arthritis, Psoriatic - blood ; Arthritis, Psoriatic - genetics ; Arthritis, Psoriatic - metabolism ; Autoimmune diseases ; Biomarkers ; Biomarkers - blood ; Blood cells ; Bone growth ; Cascades ; Cell growth ; Cytokines ; Deregulation ; Development and progression ; Disease ; DNA microarrays ; Epigenetic inheritance ; Ethylenediaminetetraacetic acid ; Female ; Gene expression ; Gene Expression Profiling ; Genes ; Humans ; Immune response ; Immunology ; Inflammation ; Jurkat Cells ; Kinases ; Ligands ; Male ; MAP kinase ; Medical research ; Medicine, Experimental ; MicroRNA ; MicroRNAs ; MicroRNAs - blood ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; miRNA ; Network analysis ; Osteogenesis ; Pathogenesis ; Patients ; Psoriasis ; Psoriatic arthritis ; Regulators ; Rheumatoid arthritis ; Ribonucleic acid ; RNA ; Signal Transduction - genetics ; Studies ; TNF inhibitors ; Transcriptome - genetics ; Tumor necrosis factor ; Tumor necrosis factor-TNF ; Wnt protein</subject><ispartof>BioMed research international, 2018-01, Vol.2018 (2018), p.1-15</ispartof><rights>Copyright © 2018 Andrea Pelosi et al.</rights><rights>COPYRIGHT 2018 John Wiley & Sons, Inc.</rights><rights>Copyright © 2018 Andrea Pelosi et al.; This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2018 Andrea Pelosi et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-e0d928c830d80a27ecc094f9b4cc3b01ae905585c60198d58ab805e4b22dd6d33</citedby><cites>FETCH-LOGICAL-c565t-e0d928c830d80a27ecc094f9b4cc3b01ae905585c60198d58ab805e4b22dd6d33</cites><orcidid>0000-0002-2869-5698 ; 0000-0001-7769-7799 ; 0000-0001-6446-2990 ; 0000-0002-6810-2172 ; 0000-0003-2308-9215</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937573/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937573/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29850558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Queiro-Silva, Ruben</contributor><creatorcontrib>Dolcino, Marzia</creatorcontrib><creatorcontrib>Antonio, Puccetti</creatorcontrib><creatorcontrib>Giuseppe, Argentino</creatorcontrib><creatorcontrib>Patuzzo, Giuseppe</creatorcontrib><creatorcontrib>Tinazzi, E.</creatorcontrib><creatorcontrib>Fiore, Piera Filomena</creatorcontrib><creatorcontrib>Lunardi, C.</creatorcontrib><creatorcontrib>Pelosi, Andrea</creatorcontrib><creatorcontrib>Moretta, Francesca</creatorcontrib><title>MicroRNA Expression Profiling in Psoriatic Arthritis</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Background. Psoriatic arthritis (PsA) is an inflammatory arthritis, characterized by bone erosions and new bone formation. MicroRNAs (miRNAs) are key regulators of the immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their role in PsA is not fully elucidated. We aimed to identify miRNA expression signatures associated with PsA and to investigate their potential implication in the disease pathogenesis. Methods. miRNA microarray was performed in blood cells of PsA patients and healthy controls. miRNA pathway analyses were performed and the global miRNA profiling was combined with transcriptome data in PsA. Deregulation of selected miRNAs was validated by real-time PCR. Results. We identified specific miRNA signatures associated with PsA patients with active disease. These miRNAs target pathways relevant in PsA, such as TNF, MAPK, and WNT signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the PsA transcriptome. miR-126-3p was the most downregulated miRNA in active patients. Noteworthy, miR-126 overexpression induced a decreased expression of genes implicated in PsA. Conclusions. This study sheds light on some epigenetic aspects of PsA identifying specific miRNAs, which may represent promising candidates as biomarkers and/or for the design of novel therapeutic strategies in PsA.</description><subject>Adult</subject><subject>Arthritis</subject><subject>Arthritis, Psoriatic - blood</subject><subject>Arthritis, Psoriatic - genetics</subject><subject>Arthritis, Psoriatic - metabolism</subject><subject>Autoimmune diseases</subject><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>Blood cells</subject><subject>Bone growth</subject><subject>Cascades</subject><subject>Cell growth</subject><subject>Cytokines</subject><subject>Deregulation</subject><subject>Development and progression</subject><subject>Disease</subject><subject>DNA microarrays</subject><subject>Epigenetic inheritance</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Genes</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Jurkat Cells</subject><subject>Kinases</subject><subject>Ligands</subject><subject>Male</subject><subject>MAP kinase</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - blood</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>Network analysis</subject><subject>Osteogenesis</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Psoriasis</subject><subject>Psoriatic arthritis</subject><subject>Regulators</subject><subject>Rheumatoid arthritis</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Signal Transduction - genetics</subject><subject>Studies</subject><subject>TNF inhibitors</subject><subject>Transcriptome - genetics</subject><subject>Tumor necrosis factor</subject><subject>Tumor necrosis factor-TNF</subject><subject>Wnt protein</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkUtPAyEURonRaFO7c22auDHR6gWGGdiYNI2vxFeMrgnDMC3NdKgw9fHvZdJaHyvZAOHkcO_9ENrDcIIxY6cEMD_NKDDKYQN1CMXJIMUJ3lyfKd1BvRCmEBfHKYh0G-0QwRkwxjsoubXau8e7Yf_8fe5NCNbV_QfvSlvZety38RKct6qxuj_0zcTbxoZdtFWqKpjeau-i54vzp9HV4Ob-8no0vBlolrJmYKAQhGtOoeCgSGa0BpGUIk-0pjlgZURbBNMpYMELxlXOgZkkJ6Qo0oLSLjpbeueLfGYKberGq0rOvZ0p_yGdsvL3S20ncuxeJRM0Y1krOFwJvHtZmNDImQ3aVJWqjVsESSDJBMGEQkQP_qBTt_B1bC9SlBGC4zy_qbGqjLR16eK_upXKYUpwJjgHHKnjJRVHG4I35bpkDLLNTba5yVVuEd__2eYa_kopAkdLYGLrQr3Zf-pMZEypvmlMOOWCfgKhYKbv</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Dolcino, Marzia</creator><creator>Antonio, Puccetti</creator><creator>Giuseppe, Argentino</creator><creator>Patuzzo, Giuseppe</creator><creator>Tinazzi, E.</creator><creator>Fiore, Piera Filomena</creator><creator>Lunardi, C.</creator><creator>Pelosi, Andrea</creator><creator>Moretta, Francesca</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2869-5698</orcidid><orcidid>https://orcid.org/0000-0001-7769-7799</orcidid><orcidid>https://orcid.org/0000-0001-6446-2990</orcidid><orcidid>https://orcid.org/0000-0002-6810-2172</orcidid><orcidid>https://orcid.org/0000-0003-2308-9215</orcidid></search><sort><creationdate>20180101</creationdate><title>MicroRNA Expression Profiling in Psoriatic Arthritis</title><author>Dolcino, Marzia ; Antonio, Puccetti ; Giuseppe, Argentino ; Patuzzo, Giuseppe ; Tinazzi, E. ; Fiore, Piera Filomena ; Lunardi, C. ; Pelosi, Andrea ; Moretta, Francesca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-e0d928c830d80a27ecc094f9b4cc3b01ae905585c60198d58ab805e4b22dd6d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Arthritis</topic><topic>Arthritis, Psoriatic - blood</topic><topic>Arthritis, Psoriatic - genetics</topic><topic>Arthritis, Psoriatic - metabolism</topic><topic>Autoimmune diseases</topic><topic>Biomarkers</topic><topic>Biomarkers - blood</topic><topic>Blood cells</topic><topic>Bone growth</topic><topic>Cascades</topic><topic>Cell growth</topic><topic>Cytokines</topic><topic>Deregulation</topic><topic>Development and progression</topic><topic>Disease</topic><topic>DNA microarrays</topic><topic>Epigenetic inheritance</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Genes</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Jurkat Cells</topic><topic>Kinases</topic><topic>Ligands</topic><topic>Male</topic><topic>MAP kinase</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - blood</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>miRNA</topic><topic>Network analysis</topic><topic>Osteogenesis</topic><topic>Pathogenesis</topic><topic>Patients</topic><topic>Psoriasis</topic><topic>Psoriatic arthritis</topic><topic>Regulators</topic><topic>Rheumatoid arthritis</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Signal Transduction - genetics</topic><topic>Studies</topic><topic>TNF inhibitors</topic><topic>Transcriptome - genetics</topic><topic>Tumor necrosis factor</topic><topic>Tumor necrosis factor-TNF</topic><topic>Wnt protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dolcino, Marzia</creatorcontrib><creatorcontrib>Antonio, Puccetti</creatorcontrib><creatorcontrib>Giuseppe, Argentino</creatorcontrib><creatorcontrib>Patuzzo, Giuseppe</creatorcontrib><creatorcontrib>Tinazzi, E.</creatorcontrib><creatorcontrib>Fiore, Piera Filomena</creatorcontrib><creatorcontrib>Lunardi, C.</creatorcontrib><creatorcontrib>Pelosi, Andrea</creatorcontrib><creatorcontrib>Moretta, Francesca</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dolcino, Marzia</au><au>Antonio, Puccetti</au><au>Giuseppe, Argentino</au><au>Patuzzo, Giuseppe</au><au>Tinazzi, E.</au><au>Fiore, Piera Filomena</au><au>Lunardi, C.</au><au>Pelosi, Andrea</au><au>Moretta, Francesca</au><au>Queiro-Silva, Ruben</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA Expression Profiling in Psoriatic Arthritis</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>2018</volume><issue>2018</issue><spage>1</spage><epage>15</epage><pages>1-15</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Background. Psoriatic arthritis (PsA) is an inflammatory arthritis, characterized by bone erosions and new bone formation. MicroRNAs (miRNAs) are key regulators of the immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their role in PsA is not fully elucidated. We aimed to identify miRNA expression signatures associated with PsA and to investigate their potential implication in the disease pathogenesis. Methods. miRNA microarray was performed in blood cells of PsA patients and healthy controls. miRNA pathway analyses were performed and the global miRNA profiling was combined with transcriptome data in PsA. Deregulation of selected miRNAs was validated by real-time PCR. Results. We identified specific miRNA signatures associated with PsA patients with active disease. These miRNAs target pathways relevant in PsA, such as TNF, MAPK, and WNT signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the PsA transcriptome. miR-126-3p was the most downregulated miRNA in active patients. Noteworthy, miR-126 overexpression induced a decreased expression of genes implicated in PsA. Conclusions. This study sheds light on some epigenetic aspects of PsA identifying specific miRNAs, which may represent promising candidates as biomarkers and/or for the design of novel therapeutic strategies in PsA.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>29850558</pmid><doi>10.1155/2018/7305380</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-2869-5698</orcidid><orcidid>https://orcid.org/0000-0001-7769-7799</orcidid><orcidid>https://orcid.org/0000-0001-6446-2990</orcidid><orcidid>https://orcid.org/0000-0002-6810-2172</orcidid><orcidid>https://orcid.org/0000-0003-2308-9215</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Arthritis Arthritis, Psoriatic - blood Arthritis, Psoriatic - genetics Arthritis, Psoriatic - metabolism Autoimmune diseases Biomarkers Biomarkers - blood Blood cells Bone growth Cascades Cell growth Cytokines Deregulation Development and progression Disease DNA microarrays Epigenetic inheritance Ethylenediaminetetraacetic acid Female Gene expression Gene Expression Profiling Genes Humans Immune response Immunology Inflammation Jurkat Cells Kinases Ligands Male MAP kinase Medical research Medicine, Experimental MicroRNA MicroRNAs MicroRNAs - blood MicroRNAs - genetics MicroRNAs - metabolism Middle Aged miRNA Network analysis Osteogenesis Pathogenesis Patients Psoriasis Psoriatic arthritis Regulators Rheumatoid arthritis Ribonucleic acid RNA Signal Transduction - genetics Studies TNF inhibitors Transcriptome - genetics Tumor necrosis factor Tumor necrosis factor-TNF Wnt protein |
| title | MicroRNA Expression Profiling in Psoriatic Arthritis |
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