Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro

Abstract In type I CRISPR-Cas systems, primed adaptation of new spacers into CRISPR arrays occurs when the effector Cascade-crRNA complex recognizes imperfectly matched targets that are not subject to efficient CRISPR interference. Thus, primed adaptation allows cells to acquire additional protectio...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Nucleic acids research 2018-05, Vol.46 (8), p.4087-4098
Hauptverfasser:	Krivoy, Andrey, Rutkauskas, Marius, Kuznedelov, Konstantin, Musharova, Olga, Rouillon, Christophe, Severinov, Konstantin, Seidel, Ralf
Format:	Artikel
Sprache:	eng
Schlagworte:	Molecular Biology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!