An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
A number of methods have been described to establish NK/T cell lines from patients with lymphoma or lymphoproliferative syndrome. These methods employed feeder cells, purified NK or T cells with as much as 10 mL of blood, or a high-dose of IL-2. This study presents a new method with a powerful and s...
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| Veröffentlicht in: | Journal of Visualized Experiments 2018-03 (133) |
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| creator | Xu, Chongfeng Ai, Junhong Zhang, Qingxun Li, Ting Wu, Xiaorong Xie, Zhengde Duan, Ziyuan |
| description | A number of methods have been described to establish NK/T cell lines from patients with lymphoma or lymphoproliferative syndrome. These methods employed feeder cells, purified NK or T cells with as much as 10 mL of blood, or a high-dose of IL-2. This study presents a new method with a powerful and simple strategy to establish NK and T cell lines by culturing the peripheral blood mononuclear cells (PBMC) with the addition of recombinant human IL-2 (rhIL-2), and uses as little as 2 mL of whole blood. The cells can proliferate quickly in two weeks and be maintained for more than 3 months. With this method, 7 NK or T cell lines have been established with a high success rate. This method is simple, reliable, and applicable to establishing cell lines from more cases of CAEBV or NK/T cell lymphoma. |
| doi_str_mv | 10.3791/56515 |
| format | Article |
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These methods employed feeder cells, purified NK or T cells with as much as 10 mL of blood, or a high-dose of IL-2. This study presents a new method with a powerful and simple strategy to establish NK and T cell lines by culturing the peripheral blood mononuclear cells (PBMC) with the addition of recombinant human IL-2 (rhIL-2), and uses as little as 2 mL of whole blood. The cells can proliferate quickly in two weeks and be maintained for more than 3 months. With this method, 7 NK or T cell lines have been established with a high success rate. 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These methods employed feeder cells, purified NK or T cells with as much as 10 mL of blood, or a high-dose of IL-2. This study presents a new method with a powerful and simple strategy to establish NK and T cell lines by culturing the peripheral blood mononuclear cells (PBMC) with the addition of recombinant human IL-2 (rhIL-2), and uses as little as 2 mL of whole blood. The cells can proliferate quickly in two weeks and be maintained for more than 3 months. With this method, 7 NK or T cell lines have been established with a high success rate. This method is simple, reliable, and applicable to establishing cell lines from more cases of CAEBV or NK/T cell lymphoma.</description><subject>Chronic Disease</subject><subject>Epstein-Barr Virus Infections - genetics</subject><subject>Epstein-Barr Virus Infections - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Immunology and Infection</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Male</subject><subject>Receptors, Antigen, T-Cell - metabolism</subject><issn>1940-087X</issn><issn>1940-087X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9vEzEQxS0EakvbL8AB-YLEZcFe_9n1BSlEoa1IWyRaxM3yOjbraNcOtjeol3523CZU5TQjvd-8Gc0D4BSjD6QR-CPjDLMX4AgLiirUNj9fPusPweuU1gjxGrH2ABzWgrNW1PQI3M88XFjrtDM-Q-VX8LsbN4OBlyb3YQVzgIuUVTe41MOrr4_EDZybYYBL502CNoYRflP5YT7BPy73cN7H4J2GM53d1sDFJmXjfPVZxQh_uDgleOGtKWLwJ-CVVUMyp_t6DG6_LG7m59Xy-uxiPltWmlCeK6sb0RnUGkQ7TjgXmuim6yjFzDYM41q0ulVaYaO57oqMGsJpzUXDO0KFJcfg0853M3WjWelybFSD3EQ3qngng3Lyf8W7Xv4KW8kEIXVDi8H7vUEMvyeTshxd0uUNypswJVmjmrWIYIYK-m6H6hhSisY-rcFIPmQlH7Mq3NvnNz1R_8IpwJsdsA5bI9dhir78aD_9F42Cl7U</recordid><startdate>20180330</startdate><enddate>20180330</enddate><creator>Xu, Chongfeng</creator><creator>Ai, Junhong</creator><creator>Zhang, Qingxun</creator><creator>Li, Ting</creator><creator>Wu, Xiaorong</creator><creator>Xie, Zhengde</creator><creator>Duan, Ziyuan</creator><general>MyJove Corporation</general><scope>BJXJS</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180330</creationdate><title>An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection</title><author>Xu, Chongfeng ; Ai, Junhong ; Zhang, Qingxun ; Li, Ting ; Wu, Xiaorong ; Xie, Zhengde ; Duan, Ziyuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c346t-fc79be08e04b63669c3c7bb4415f7511298c8aca1ec6cb69c0736426976b349f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Chronic Disease</topic><topic>Epstein-Barr Virus Infections - genetics</topic><topic>Epstein-Barr Virus Infections - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Immunology and Infection</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Male</topic><topic>Receptors, Antigen, T-Cell - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Chongfeng</creatorcontrib><creatorcontrib>Ai, Junhong</creatorcontrib><creatorcontrib>Zhang, Qingxun</creatorcontrib><creatorcontrib>Li, Ting</creatorcontrib><creatorcontrib>Wu, Xiaorong</creatorcontrib><creatorcontrib>Xie, Zhengde</creatorcontrib><creatorcontrib>Duan, Ziyuan</creatorcontrib><collection>JoVE Journal: Immunology and Infection</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Visualized Experiments</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Xu, Chongfeng</au><au>Ai, Junhong</au><au>Zhang, Qingxun</au><au>Li, Ting</au><au>Wu, Xiaorong</au><au>Xie, Zhengde</au><au>Duan, Ziyuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection</atitle><jtitle>Journal of Visualized Experiments</jtitle><addtitle>J Vis Exp</addtitle><date>2018-03-30</date><risdate>2018</risdate><issue>133</issue><issn>1940-087X</issn><eissn>1940-087X</eissn><abstract>A number of methods have been described to establish NK/T cell lines from patients with lymphoma or lymphoproliferative syndrome. 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| subjects | Chronic Disease Epstein-Barr Virus Infections - genetics Epstein-Barr Virus Infections - metabolism Female Humans Immunology and Infection Killer Cells, Natural - metabolism Male Receptors, Antigen, T-Cell - metabolism |
| title | An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection |
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