Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues
We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents - taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both component...
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| Veröffentlicht in: | Chemical science (Cambridge) 2018-04, Vol.9 (13), p.3324-3334 |
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| creator | Lukinavičius, Gražvydas Mitronova, Gyuzel Y Schnorrenberg, Sebastian Butkevich, Alexey N Barthel, Hannah Belov, Vladimir N Hell, Stefan W |
| description | We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents - taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly (
) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae. |
| doi_str_mv | 10.1039/c7sc05334g |
| format | Article |
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) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae.</description><identifier>ISSN: 2041-6520</identifier><identifier>EISSN: 2041-6539</identifier><identifier>DOI: 10.1039/c7sc05334g</identifier><identifier>PMID: 29780462</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Chemistry ; Chemotherapy ; Chitin ; Cytotoxicity ; Fibroblasts ; Fluorescence ; Fluorescent dyes ; Fruit flies ; Isomers ; Larvae ; Microscopy ; Periodic variations ; Stimulated emission ; Toxicity</subject><ispartof>Chemical science (Cambridge), 2018-04, Vol.9 (13), p.3324-3334</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><rights>This journal is © The Royal Society of Chemistry 2018 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-1b9af5f72cea9059978d92fc1742ff9b0c421877e6c6280e3f649157be4f700b3</citedby><cites>FETCH-LOGICAL-c406t-1b9af5f72cea9059978d92fc1742ff9b0c421877e6c6280e3f649157be4f700b3</cites><orcidid>0000-0002-9638-5077 ; 0000-0002-7741-4653 ; 0000-0002-7176-1793 ; 0000-0003-4376-6981 ; 0000-0002-8076-2237 ; 0000-0002-7458-7508</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932598/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932598/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29780462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lukinavičius, Gražvydas</creatorcontrib><creatorcontrib>Mitronova, Gyuzel Y</creatorcontrib><creatorcontrib>Schnorrenberg, Sebastian</creatorcontrib><creatorcontrib>Butkevich, Alexey N</creatorcontrib><creatorcontrib>Barthel, Hannah</creatorcontrib><creatorcontrib>Belov, Vladimir N</creatorcontrib><creatorcontrib>Hell, Stefan W</creatorcontrib><title>Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues</title><title>Chemical science (Cambridge)</title><addtitle>Chem Sci</addtitle><description>We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents - taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly (
) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae.</description><subject>Chemistry</subject><subject>Chemotherapy</subject><subject>Chitin</subject><subject>Cytotoxicity</subject><subject>Fibroblasts</subject><subject>Fluorescence</subject><subject>Fluorescent dyes</subject><subject>Fruit flies</subject><subject>Isomers</subject><subject>Larvae</subject><subject>Microscopy</subject><subject>Periodic variations</subject><subject>Stimulated emission</subject><subject>Toxicity</subject><issn>2041-6520</issn><issn>2041-6539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkU1LAzEQhoMoWmov_gBZ8CLCar5201wEKbYKBQ_qOWTTpI1sN2uyKfTfm7W1qLnMDPPk5Z0ZAC4QvEWQ8DvFgoIFIXR5BAYYUpSXBeHHhxzDMzAK4QOmRwgqMDsFZ5izMaQlHoDNtI7O66B002WLrQ6ZbBZZ612VUuN8FmKrfZ4IV8fOuiZbW-VdUK7dZs7sqi5Wsd5_7bxUK-360jZZbTe2WWZK1_W-bUOIOpyDEyProEf7OATv08e3yVM-f5k9Tx7muaKw7HJUcWkKw7DSksOCJ9sLjo1CjGJjeAUVxWjMmC5VicdQE1NSjgpWaWoYhBUZgvudbhurtV70U3pZi9bbtfRb4aQVfzuNXYml24iCE1zwcRK43gt495mMd2JtQz-ObLSLQaQtY0wopjyhV__QDxd9k8ZLFOKIIc5Rom52VL_F4LU5mEFQ9BcVE_Y6-b7oLMGXv-0f0J_7kS-4Q54w</recordid><startdate>20180407</startdate><enddate>20180407</enddate><creator>Lukinavičius, Gražvydas</creator><creator>Mitronova, Gyuzel Y</creator><creator>Schnorrenberg, Sebastian</creator><creator>Butkevich, Alexey N</creator><creator>Barthel, Hannah</creator><creator>Belov, Vladimir N</creator><creator>Hell, Stefan W</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9638-5077</orcidid><orcidid>https://orcid.org/0000-0002-7741-4653</orcidid><orcidid>https://orcid.org/0000-0002-7176-1793</orcidid><orcidid>https://orcid.org/0000-0003-4376-6981</orcidid><orcidid>https://orcid.org/0000-0002-8076-2237</orcidid><orcidid>https://orcid.org/0000-0002-7458-7508</orcidid></search><sort><creationdate>20180407</creationdate><title>Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues</title><author>Lukinavičius, Gražvydas ; Mitronova, Gyuzel Y ; Schnorrenberg, Sebastian ; Butkevich, Alexey N ; Barthel, Hannah ; Belov, Vladimir N ; Hell, Stefan W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-1b9af5f72cea9059978d92fc1742ff9b0c421877e6c6280e3f649157be4f700b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Chemistry</topic><topic>Chemotherapy</topic><topic>Chitin</topic><topic>Cytotoxicity</topic><topic>Fibroblasts</topic><topic>Fluorescence</topic><topic>Fluorescent dyes</topic><topic>Fruit flies</topic><topic>Isomers</topic><topic>Larvae</topic><topic>Microscopy</topic><topic>Periodic variations</topic><topic>Stimulated emission</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lukinavičius, Gražvydas</creatorcontrib><creatorcontrib>Mitronova, Gyuzel Y</creatorcontrib><creatorcontrib>Schnorrenberg, Sebastian</creatorcontrib><creatorcontrib>Butkevich, Alexey N</creatorcontrib><creatorcontrib>Barthel, Hannah</creatorcontrib><creatorcontrib>Belov, Vladimir N</creatorcontrib><creatorcontrib>Hell, Stefan W</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chemical science (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lukinavičius, Gražvydas</au><au>Mitronova, Gyuzel Y</au><au>Schnorrenberg, Sebastian</au><au>Butkevich, Alexey N</au><au>Barthel, Hannah</au><au>Belov, Vladimir N</au><au>Hell, Stefan W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues</atitle><jtitle>Chemical science (Cambridge)</jtitle><addtitle>Chem Sci</addtitle><date>2018-04-07</date><risdate>2018</risdate><volume>9</volume><issue>13</issue><spage>3324</spage><epage>3334</epage><pages>3324-3334</pages><issn>2041-6520</issn><eissn>2041-6539</eissn><abstract>We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents - taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly (
) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29780462</pmid><doi>10.1039/c7sc05334g</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9638-5077</orcidid><orcidid>https://orcid.org/0000-0002-7741-4653</orcidid><orcidid>https://orcid.org/0000-0002-7176-1793</orcidid><orcidid>https://orcid.org/0000-0003-4376-6981</orcidid><orcidid>https://orcid.org/0000-0002-8076-2237</orcidid><orcidid>https://orcid.org/0000-0002-7458-7508</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Chemistry Chemotherapy Chitin Cytotoxicity Fibroblasts Fluorescence Fluorescent dyes Fruit flies Isomers Larvae Microscopy Periodic variations Stimulated emission Toxicity |
| title | Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues |
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