Defeating Major Contaminants in Fe3+- Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphopeptide Enrichment

Here we demonstrate that biomolecular contaminants, such as nucleic acid molecules, can seriously interfere with immobilized metal ion affinity chromatography (IMAC)-based phosphopeptide enrichments. We address and largely solve this issue, developing a robust protocol implementing methanol/chlorofo...

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Veröffentlicht in:	Molecular & cellular proteomics 2018-05, Vol.17 (5), p.1028-1034
Hauptverfasser:	Potel, Clement M., Lin, Miao-Hsia, Heck, Albert J.R., Lemeer, Simone
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Affinity chromatography Chloroform Chromatography Contaminants Deoxyribonucleic acid DNA E coli Enrichment Iron Metal ions Molecular chains Ribonucleic acid RNA Sample preparation Technological Innovation and Resources
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