Differential Effects of Partial Hepatectomy and Carbon Tetrachloride Administration on Induction of Liver Cell Foci in a Model for Detection of Initiation Activity
Differential effects of partial hepatectomy (PH) and carbon tetrachloride (CC14) administration on induction of glutathione S‐transferase placental form (GST‐P)‐positive foci were investigated in a model for detection of initiation activity. Firstly, we surveyed cell proliferation kinetics and fluct...
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| Veröffentlicht in: | Cancer science 2001-10, Vol.92 (10), p.1018-1025 |
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| creator | Sakai, Hiroki Tsukamoto, Tetsuya Yamamoto, Masami Shirai, Norimitsu lidaka, Takeshi Yanai, Tokuma Masegi, Toshiaki Tatematsu, Masae |
| description | Differential effects of partial hepatectomy (PH) and carbon tetrachloride (CC14) administration on induction of glutathione S‐transferase placental form (GST‐P)‐positive foci were investigated in a model for detection of initiation activity. Firstly, we surveyed cell proliferation kinetics and fluctuation in cytochrome P450 (CYP) mRNA levels by means of relative‐quantitative real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) and CYP 2E1 apoprotein amount by immuno‐blotting (experiment I) after PH or CC14 administration. Next, to assess the interrelationships among cell proliferation, fluctuation of CYPs after PH or CC14 administration and induction of liver cell foci, the non‐hepatocarcinogen, 1,2‐dimethylhydrazine (DMH) was administered to 7‐week‐old male F344 rats and initiated populations were selected using the resistant hepatocyte model (experiment II). In experiment I, the values of all CYP isozyme mRNAs after PH or CC14 administration were drastically decreased at the 12‐h tune point. From 72 h, mRNAs for all CYP isozymes began increasing, with complete recovery after 7 days. The CYP 2E1 apoprotein content in the PH group fluctuated weakly, whereas in the CC14 group it had decreased rapidly after 12 h and was still low at the 48 h point. In experiment II, induction of GST‐P‐positive foci was related to cell kinetics in the PH group, with about a 6‐h time lag between tune for carcinogen administration giving greatest induction of GST‐P‐positive foci and peaks in bromodeoxyuridine (BrdU) labeling, presumably due to the necessity for bioactivation of DMH. With CC14 administration, induction of foci appeared dependent on the recovery of CYP 2E1. In conclusion, PH was able to induce cell proliferation with maintenance of CYP 2E1, therefore being advantageous for induction of liver cell foci in models to detect initiation activity. |
| doi_str_mv | 10.1111/j.1349-7006.2001.tb01055.x |
| format | Article |
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Firstly, we surveyed cell proliferation kinetics and fluctuation in cytochrome P450 (CYP) mRNA levels by means of relative‐quantitative real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) and CYP 2E1 apoprotein amount by immuno‐blotting (experiment I) after PH or CC14 administration. Next, to assess the interrelationships among cell proliferation, fluctuation of CYPs after PH or CC14 administration and induction of liver cell foci, the non‐hepatocarcinogen, 1,2‐dimethylhydrazine (DMH) was administered to 7‐week‐old male F344 rats and initiated populations were selected using the resistant hepatocyte model (experiment II). In experiment I, the values of all CYP isozyme mRNAs after PH or CC14 administration were drastically decreased at the 12‐h tune point. From 72 h, mRNAs for all CYP isozymes began increasing, with complete recovery after 7 days. The CYP 2E1 apoprotein content in the PH group fluctuated weakly, whereas in the CC14 group it had decreased rapidly after 12 h and was still low at the 48 h point. In experiment II, induction of GST‐P‐positive foci was related to cell kinetics in the PH group, with about a 6‐h time lag between tune for carcinogen administration giving greatest induction of GST‐P‐positive foci and peaks in bromodeoxyuridine (BrdU) labeling, presumably due to the necessity for bioactivation of DMH. With CC14 administration, induction of foci appeared dependent on the recovery of CYP 2E1. In conclusion, PH was able to induce cell proliferation with maintenance of CYP 2E1, therefore being advantageous for induction of liver cell foci in models to detect initiation activity.</description><identifier>ISSN: 0910-5050</identifier><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>EISSN: 1876-4673</identifier><identifier>DOI: 10.1111/j.1349-7006.2001.tb01055.x</identifier><identifier>PMID: 11676851</identifier><identifier>CODEN: GANNA2</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Apoenzymes - metabolism ; Biological and medical sciences ; Blotting, Western ; Bromodeoxyuridine ; Carbon tetrachloride ; Carbon Tetrachloride - administration & dosage ; Carbon Tetrachloride - pharmacology ; Carcinogenesis, carcinogens and anticarcinogens ; Cell culture ; Cell Division - drug effects ; Cell growth ; Cell proliferation ; Cytochrome P-450 CYP2E1 - genetics ; Cytochrome P-450 CYP2E1 - metabolism ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Cytochrome P450 ; Enzyme Induction - drug effects ; Experiments ; General aspects ; Glutathione ; Glutathione Transferase - biosynthesis ; GST‐P‐positive foci ; Hepatectomy ; Hepatocytes ; Hepatocytes - cytology ; Hepatocytes - drug effects ; Hepatocytes - enzymology ; Hepatocytes - pathology ; Initiation ; Isoenzymes ; Male ; Medical sciences ; Medium‐term bioassay ; Partial hepatectomy ; Placenta ; Polymerase chain reaction ; Rats ; Rats, Inbred F344 ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA-directed DNA polymerase ; Time Factors ; Tumors</subject><ispartof>Cancer science, 2001-10, Vol.92 (10), p.1018-1025</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright John Wiley & Sons, Inc. Oct 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6558-5d80f66a569ab7e3d33d42211a36b53fe4f25984c01d9815a55f78f763a227f53</citedby><cites>FETCH-LOGICAL-c6558-5d80f66a569ab7e3d33d42211a36b53fe4f25984c01d9815a55f78f763a227f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926621/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926621/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,27901,27902,45550,45551,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14142332$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11676851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakai, Hiroki</creatorcontrib><creatorcontrib>Tsukamoto, Tetsuya</creatorcontrib><creatorcontrib>Yamamoto, Masami</creatorcontrib><creatorcontrib>Shirai, Norimitsu</creatorcontrib><creatorcontrib>lidaka, Takeshi</creatorcontrib><creatorcontrib>Yanai, Tokuma</creatorcontrib><creatorcontrib>Masegi, Toshiaki</creatorcontrib><creatorcontrib>Tatematsu, Masae</creatorcontrib><title>Differential Effects of Partial Hepatectomy and Carbon Tetrachloride Administration on Induction of Liver Cell Foci in a Model for Detection of Initiation Activity</title><title>Cancer science</title><addtitle>Jpn J Cancer Res</addtitle><description>Differential effects of partial hepatectomy (PH) and carbon tetrachloride (CC14) administration on induction of glutathione S‐transferase placental form (GST‐P)‐positive foci were investigated in a model for detection of initiation activity. Firstly, we surveyed cell proliferation kinetics and fluctuation in cytochrome P450 (CYP) mRNA levels by means of relative‐quantitative real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) and CYP 2E1 apoprotein amount by immuno‐blotting (experiment I) after PH or CC14 administration. Next, to assess the interrelationships among cell proliferation, fluctuation of CYPs after PH or CC14 administration and induction of liver cell foci, the non‐hepatocarcinogen, 1,2‐dimethylhydrazine (DMH) was administered to 7‐week‐old male F344 rats and initiated populations were selected using the resistant hepatocyte model (experiment II). In experiment I, the values of all CYP isozyme mRNAs after PH or CC14 administration were drastically decreased at the 12‐h tune point. From 72 h, mRNAs for all CYP isozymes began increasing, with complete recovery after 7 days. The CYP 2E1 apoprotein content in the PH group fluctuated weakly, whereas in the CC14 group it had decreased rapidly after 12 h and was still low at the 48 h point. In experiment II, induction of GST‐P‐positive foci was related to cell kinetics in the PH group, with about a 6‐h time lag between tune for carcinogen administration giving greatest induction of GST‐P‐positive foci and peaks in bromodeoxyuridine (BrdU) labeling, presumably due to the necessity for bioactivation of DMH. With CC14 administration, induction of foci appeared dependent on the recovery of CYP 2E1. In conclusion, PH was able to induce cell proliferation with maintenance of CYP 2E1, therefore being advantageous for induction of liver cell foci in models to detect initiation activity.</description><subject>Animals</subject><subject>Apoenzymes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Bromodeoxyuridine</subject><subject>Carbon tetrachloride</subject><subject>Carbon Tetrachloride - administration & dosage</subject><subject>Carbon Tetrachloride - pharmacology</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Cell culture</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cytochrome P-450 CYP2E1 - genetics</subject><subject>Cytochrome P-450 CYP2E1 - metabolism</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Cytochrome P450</subject><subject>Enzyme Induction - drug effects</subject><subject>Experiments</subject><subject>General aspects</subject><subject>Glutathione</subject><subject>Glutathione Transferase - biosynthesis</subject><subject>GST‐P‐positive foci</subject><subject>Hepatectomy</subject><subject>Hepatocytes</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - enzymology</subject><subject>Hepatocytes - pathology</subject><subject>Initiation</subject><subject>Isoenzymes</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Medium‐term bioassay</subject><subject>Partial hepatectomy</subject><subject>Placenta</subject><subject>Polymerase chain reaction</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA-directed DNA polymerase</subject><subject>Time Factors</subject><subject>Tumors</subject><issn>0910-5050</issn><issn>1347-9032</issn><issn>1349-7006</issn><issn>1876-4673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVkVtv0zAYhi0EYmXwF5AF4jLBh9hJuABVXccqFcHFuLYcH5ir1C52Wtbfsz86Z402uMSyZPv9nu8gvwC8w6jEeX3clJhWbVEjxEuCEC6HDmHEWHn7DMweQ8_BDLUYFQwxdAZepbTJaI04eQnOMOY1bxiegbsLZ62Jxg9O9nCZ72pIMFj4Q8YH6crs5JDFsD1C6TVcyNgFD6_NEKW66UN02sC53jrvUpYGl4N5r7zeq9PDwrU7mAgXpu_hZVAOOg8l_Ba06aENEV6YscHErrzLfR9e8ywe3HB8DV5Y2SfzZjrPwc_L5fXiqlh__7pazNeF4ow1BdMNspxLxlvZ1YZqSnVFCMaS8o5RaypLWNtUCmHdNphJxmzd2JpTSUhtGT0Hn091d_tua7TKnxJlL3bRbWU8iiCd-Dfi3Y34FQ6CtYRzgnOB91OBGH7vTRrEJuyjzzMLQtu2aWuGaKY-nSgVQ0rR2McOGInRYLERo4tidFGMBovJYHGbk9_-PeNT6uRoBj5MgExK9jZKr1x64ipcEUpJ5r6cuD-uN8f_GEEs5kuMcEPvAfhAxTg</recordid><startdate>200110</startdate><enddate>200110</enddate><creator>Sakai, Hiroki</creator><creator>Tsukamoto, Tetsuya</creator><creator>Yamamoto, Masami</creator><creator>Shirai, Norimitsu</creator><creator>lidaka, Takeshi</creator><creator>Yanai, Tokuma</creator><creator>Masegi, Toshiaki</creator><creator>Tatematsu, Masae</creator><general>Blackwell Publishing Ltd</general><general>Japanese Cancer Association</general><general>John Wiley & Sons, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>200110</creationdate><title>Differential Effects of Partial Hepatectomy and Carbon Tetrachloride Administration on Induction of Liver Cell Foci in a Model for Detection of Initiation Activity</title><author>Sakai, Hiroki ; Tsukamoto, Tetsuya ; Yamamoto, Masami ; Shirai, Norimitsu ; lidaka, Takeshi ; Yanai, Tokuma ; Masegi, Toshiaki ; Tatematsu, Masae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6558-5d80f66a569ab7e3d33d42211a36b53fe4f25984c01d9815a55f78f763a227f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Apoenzymes - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Bromodeoxyuridine</topic><topic>Carbon tetrachloride</topic><topic>Carbon Tetrachloride - administration & dosage</topic><topic>Carbon Tetrachloride - pharmacology</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Cell culture</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Cytochrome P-450 CYP2E1 - genetics</topic><topic>Cytochrome P-450 CYP2E1 - metabolism</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Cytochrome P450</topic><topic>Enzyme Induction - drug effects</topic><topic>Experiments</topic><topic>General aspects</topic><topic>Glutathione</topic><topic>Glutathione Transferase - biosynthesis</topic><topic>GST‐P‐positive foci</topic><topic>Hepatectomy</topic><topic>Hepatocytes</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - enzymology</topic><topic>Hepatocytes - pathology</topic><topic>Initiation</topic><topic>Isoenzymes</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medium‐term bioassay</topic><topic>Partial hepatectomy</topic><topic>Placenta</topic><topic>Polymerase chain reaction</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA-directed DNA polymerase</topic><topic>Time Factors</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakai, Hiroki</creatorcontrib><creatorcontrib>Tsukamoto, Tetsuya</creatorcontrib><creatorcontrib>Yamamoto, Masami</creatorcontrib><creatorcontrib>Shirai, Norimitsu</creatorcontrib><creatorcontrib>lidaka, Takeshi</creatorcontrib><creatorcontrib>Yanai, Tokuma</creatorcontrib><creatorcontrib>Masegi, Toshiaki</creatorcontrib><creatorcontrib>Tatematsu, Masae</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakai, Hiroki</au><au>Tsukamoto, Tetsuya</au><au>Yamamoto, Masami</au><au>Shirai, Norimitsu</au><au>lidaka, Takeshi</au><au>Yanai, Tokuma</au><au>Masegi, Toshiaki</au><au>Tatematsu, Masae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Effects of Partial Hepatectomy and Carbon Tetrachloride Administration on Induction of Liver Cell Foci in a Model for Detection of Initiation Activity</atitle><jtitle>Cancer science</jtitle><addtitle>Jpn J Cancer Res</addtitle><date>2001-10</date><risdate>2001</risdate><volume>92</volume><issue>10</issue><spage>1018</spage><epage>1025</epage><pages>1018-1025</pages><issn>0910-5050</issn><issn>1347-9032</issn><eissn>1349-7006</eissn><eissn>1876-4673</eissn><coden>GANNA2</coden><abstract>Differential effects of partial hepatectomy (PH) and carbon tetrachloride (CC14) administration on induction of glutathione S‐transferase placental form (GST‐P)‐positive foci were investigated in a model for detection of initiation activity. Firstly, we surveyed cell proliferation kinetics and fluctuation in cytochrome P450 (CYP) mRNA levels by means of relative‐quantitative real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) and CYP 2E1 apoprotein amount by immuno‐blotting (experiment I) after PH or CC14 administration. Next, to assess the interrelationships among cell proliferation, fluctuation of CYPs after PH or CC14 administration and induction of liver cell foci, the non‐hepatocarcinogen, 1,2‐dimethylhydrazine (DMH) was administered to 7‐week‐old male F344 rats and initiated populations were selected using the resistant hepatocyte model (experiment II). In experiment I, the values of all CYP isozyme mRNAs after PH or CC14 administration were drastically decreased at the 12‐h tune point. From 72 h, mRNAs for all CYP isozymes began increasing, with complete recovery after 7 days. The CYP 2E1 apoprotein content in the PH group fluctuated weakly, whereas in the CC14 group it had decreased rapidly after 12 h and was still low at the 48 h point. In experiment II, induction of GST‐P‐positive foci was related to cell kinetics in the PH group, with about a 6‐h time lag between tune for carcinogen administration giving greatest induction of GST‐P‐positive foci and peaks in bromodeoxyuridine (BrdU) labeling, presumably due to the necessity for bioactivation of DMH. With CC14 administration, induction of foci appeared dependent on the recovery of CYP 2E1. In conclusion, PH was able to induce cell proliferation with maintenance of CYP 2E1, therefore being advantageous for induction of liver cell foci in models to detect initiation activity.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11676851</pmid><doi>10.1111/j.1349-7006.2001.tb01055.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Apoenzymes - metabolism Biological and medical sciences Blotting, Western Bromodeoxyuridine Carbon tetrachloride Carbon Tetrachloride - administration & dosage Carbon Tetrachloride - pharmacology Carcinogenesis, carcinogens and anticarcinogens Cell culture Cell Division - drug effects Cell growth Cell proliferation Cytochrome P-450 CYP2E1 - genetics Cytochrome P-450 CYP2E1 - metabolism Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Cytochrome P450 Enzyme Induction - drug effects Experiments General aspects Glutathione Glutathione Transferase - biosynthesis GST‐P‐positive foci Hepatectomy Hepatocytes Hepatocytes - cytology Hepatocytes - drug effects Hepatocytes - enzymology Hepatocytes - pathology Initiation Isoenzymes Male Medical sciences Medium‐term bioassay Partial hepatectomy Placenta Polymerase chain reaction Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism RNA-directed DNA polymerase Time Factors Tumors |
| title | Differential Effects of Partial Hepatectomy and Carbon Tetrachloride Administration on Induction of Liver Cell Foci in a Model for Detection of Initiation Activity |
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