Genotype-phenotype relationship of CCL5 in pulmonary tuberculosis infection in Sahariya tribe: A pilot study
Background & objectives: Sahariya, a primitive tribe of Central India, has shown significantly increased incidence of pulmonary tuberculosis (PTB). Our previous study on Sahariya showed a significant association of −403G>A single nucleotide polymorphism (SNP) of CCL5 with susceptibility to PT...
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| Veröffentlicht in: | Indian journal of medical research (New Delhi, India : 1994) India : 1994), 2017-12, Vol.146 (6), p.768-773 |
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| creator | Mishra, Gunja Poojary, Satish Jain, Sanjay Tiwari, Pramod |
| description | Background & objectives: Sahariya, a primitive tribe of Central India, has shown significantly increased incidence of pulmonary tuberculosis (PTB). Our previous study on Sahariya showed a significant association of −403G>A single nucleotide polymorphism (SNP) of CCL5 with susceptibility to PTB. Hence, this study was aimed to analyze a genotype-phenotype relationship of this disease-associated SNP to develop a potential diagnostic marker for TB in this tribe.
Methods: The present study was carried out on 70 plasma samples from Sahariya tribe, wherein the plasma CCL5 level was determined using a commercially available ELISA kit.
Results: The level of CCL5 decreased significantly in patients who were on therapy/completed their therapy [inactive TB patient/inactive PTB (IPTB)], particularly with AA genotype of −403G>A (P=0.046). The level, with AA genotype, was also found to gradually decrease in sputum 3+ and 1+/2+ than in sputum-negative samples. Similarly, the CCL5 level was found to be higher in sputum-positive/active TB patients than in IPTB group and healthy controls.
Interpretation & conclusions: Our results suggested that the CCL5 level was influenced collectively not only by the genotypes of −403G>A SNP and bacillary load but also by the treatment. Thus, CCL5 may be considered for the development of a diagnostic marker and also as an indicator of recovery. |
| doi_str_mv | 10.4103/ijmr.IJMR_1582_15 |
| format | Article |
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Methods: The present study was carried out on 70 plasma samples from Sahariya tribe, wherein the plasma CCL5 level was determined using a commercially available ELISA kit.
Results: The level of CCL5 decreased significantly in patients who were on therapy/completed their therapy [inactive TB patient/inactive PTB (IPTB)], particularly with AA genotype of −403G>A (P=0.046). The level, with AA genotype, was also found to gradually decrease in sputum 3+ and 1+/2+ than in sputum-negative samples. Similarly, the CCL5 level was found to be higher in sputum-positive/active TB patients than in IPTB group and healthy controls.
Interpretation & conclusions: Our results suggested that the CCL5 level was influenced collectively not only by the genotypes of −403G>A SNP and bacillary load but also by the treatment. Thus, CCL5 may be considered for the development of a diagnostic marker and also as an indicator of recovery.</description><identifier>ISSN: 0971-5916</identifier><identifier>DOI: 10.4103/ijmr.IJMR_1582_15</identifier><identifier>PMID: 29664036</identifier><language>eng</language><publisher>India: Wolters Kluwer India Pvt. Ltd</publisher><subject>Adult ; Cardiovascular disease ; Chemokine CCL5 - genetics ; Chemokines ; Female ; Genetic aspects ; Genetic Association Studies ; Genetic Predisposition to Disease ; Genomics ; Genotype ; Genotype & phenotype ; Genotypes ; Haplotypes ; Health aspects ; HIV ; Human immunodeficiency virus ; Humans ; Infections ; Male ; Medical research ; Middle Aged ; Original ; Pathogenesis ; Phenotype ; Phenotypes ; Plasma ; Polymorphism ; Polymorphism, Single Nucleotide - genetics ; Pulmonary tuberculosis ; Sample size ; Software ; Sputum - drug effects ; Sputum - microbiology ; Studies ; Tuberculosis ; Tuberculosis, Pulmonary - blood ; Tuberculosis, Pulmonary - drug therapy ; Tuberculosis, Pulmonary - genetics ; Tuberculosis, Pulmonary - microbiology</subject><ispartof>Indian journal of medical research (New Delhi, India : 1994), 2017-12, Vol.146 (6), p.768-773</ispartof><rights>COPYRIGHT 2017 Medknow Publications and Media Pvt. Ltd.</rights><rights>2017. This work is published under https://creativecommons.org/licenses/by-nc-sa/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright: © 2017 Indian Journal of Medical Research 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554e-cba46826b62a9f4037db08d650089bc36d9936870ce82d268b94c2fd7a8ae4c53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926349/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926349/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29664036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mishra, Gunja</creatorcontrib><creatorcontrib>Poojary, Satish</creatorcontrib><creatorcontrib>Jain, Sanjay</creatorcontrib><creatorcontrib>Tiwari, Pramod</creatorcontrib><title>Genotype-phenotype relationship of CCL5 in pulmonary tuberculosis infection in Sahariya tribe: A pilot study</title><title>Indian journal of medical research (New Delhi, India : 1994)</title><addtitle>Indian J Med Res</addtitle><description>Background & objectives: Sahariya, a primitive tribe of Central India, has shown significantly increased incidence of pulmonary tuberculosis (PTB). Our previous study on Sahariya showed a significant association of −403G>A single nucleotide polymorphism (SNP) of CCL5 with susceptibility to PTB. Hence, this study was aimed to analyze a genotype-phenotype relationship of this disease-associated SNP to develop a potential diagnostic marker for TB in this tribe.
Methods: The present study was carried out on 70 plasma samples from Sahariya tribe, wherein the plasma CCL5 level was determined using a commercially available ELISA kit.
Results: The level of CCL5 decreased significantly in patients who were on therapy/completed their therapy [inactive TB patient/inactive PTB (IPTB)], particularly with AA genotype of −403G>A (P=0.046). The level, with AA genotype, was also found to gradually decrease in sputum 3+ and 1+/2+ than in sputum-negative samples. Similarly, the CCL5 level was found to be higher in sputum-positive/active TB patients than in IPTB group and healthy controls.
Interpretation & conclusions: Our results suggested that the CCL5 level was influenced collectively not only by the genotypes of −403G>A SNP and bacillary load but also by the treatment. Thus, CCL5 may be considered for the development of a diagnostic marker and also as an indicator of recovery.</description><subject>Adult</subject><subject>Cardiovascular disease</subject><subject>Chemokine CCL5 - genetics</subject><subject>Chemokines</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Genetic Association Studies</subject><subject>Genetic Predisposition to Disease</subject><subject>Genomics</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Haplotypes</subject><subject>Health aspects</subject><subject>HIV</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Infections</subject><subject>Male</subject><subject>Medical research</subject><subject>Middle Aged</subject><subject>Original</subject><subject>Pathogenesis</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Plasma</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Pulmonary tuberculosis</subject><subject>Sample size</subject><subject>Software</subject><subject>Sputum - drug effects</subject><subject>Sputum - microbiology</subject><subject>Studies</subject><subject>Tuberculosis</subject><subject>Tuberculosis, Pulmonary - blood</subject><subject>Tuberculosis, Pulmonary - drug therapy</subject><subject>Tuberculosis, Pulmonary - genetics</subject><subject>Tuberculosis, Pulmonary - microbiology</subject><issn>0971-5916</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kl2P1CAUhnuhcdfRH-CNIfHGm46UAi1emEyadV0zxsSPa0Lp6Q6zFCq0Tubfy2RmN7NGQwIEnvOec-DNslcFXtICl-_MdgjLm89fvsmC1SRNT7JLLKoiZ6LgF9nzGLcYF4JU4ll2QQTnFJf8MrPX4Py0HyEfN6cdCmDVZLyLGzMi36OmWTNkHBpnO3inwh5NcwtBz9ZHE9NND_rAH5jvaqOC2Ss0BdPCe7RCo7F-QnGau_2L7GmvbISXp3WR_fx49aP5lK-_Xt80q3WuGaOQ61ZRXhPecqJEn-qsuhbXHWcY16LVJe-EKHldYQ016QivW0E16btK1QqoZuUi-3DUHed2gE6Dm4KycgxmSNVLr4x8fOPMRt7635IJwksqksDbk0Dwv2aIkxxM1GCtcuDnKAkmnBYVoSShb_5Ct34OLrUnCUk_gRmrzqhbZUGmF_Mprz6IyhUrWSFomTIvsuU_qDQ6GIz2DnqTzh8FFMcAHXyMAfqHHgssD7aQB1vIc1ukmNfnj_MQce-JBDRHYOftBCHe2XkHQSb2zvnd_5VlxWt5b6fyD73qztA</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Mishra, Gunja</creator><creator>Poojary, Satish</creator><creator>Jain, Sanjay</creator><creator>Tiwari, Pramod</creator><general>Wolters Kluwer India Pvt. 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Our previous study on Sahariya showed a significant association of −403G>A single nucleotide polymorphism (SNP) of CCL5 with susceptibility to PTB. Hence, this study was aimed to analyze a genotype-phenotype relationship of this disease-associated SNP to develop a potential diagnostic marker for TB in this tribe.
Methods: The present study was carried out on 70 plasma samples from Sahariya tribe, wherein the plasma CCL5 level was determined using a commercially available ELISA kit.
Results: The level of CCL5 decreased significantly in patients who were on therapy/completed their therapy [inactive TB patient/inactive PTB (IPTB)], particularly with AA genotype of −403G>A (P=0.046). The level, with AA genotype, was also found to gradually decrease in sputum 3+ and 1+/2+ than in sputum-negative samples. Similarly, the CCL5 level was found to be higher in sputum-positive/active TB patients than in IPTB group and healthy controls.
Interpretation & conclusions: Our results suggested that the CCL5 level was influenced collectively not only by the genotypes of −403G>A SNP and bacillary load but also by the treatment. Thus, CCL5 may be considered for the development of a diagnostic marker and also as an indicator of recovery.</abstract><cop>India</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><pmid>29664036</pmid><doi>10.4103/ijmr.IJMR_1582_15</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Cardiovascular disease Chemokine CCL5 - genetics Chemokines Female Genetic aspects Genetic Association Studies Genetic Predisposition to Disease Genomics Genotype Genotype & phenotype Genotypes Haplotypes Health aspects HIV Human immunodeficiency virus Humans Infections Male Medical research Middle Aged Original Pathogenesis Phenotype Phenotypes Plasma Polymorphism Polymorphism, Single Nucleotide - genetics Pulmonary tuberculosis Sample size Software Sputum - drug effects Sputum - microbiology Studies Tuberculosis Tuberculosis, Pulmonary - blood Tuberculosis, Pulmonary - drug therapy Tuberculosis, Pulmonary - genetics Tuberculosis, Pulmonary - microbiology |
| title | Genotype-phenotype relationship of CCL5 in pulmonary tuberculosis infection in Sahariya tribe: A pilot study |
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